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[1]魏翠翠 央青卓玛 陶勇** 顾文智 郑德聪 何晓红.瘤胃菌与乳酸菌共培养利用葡萄糖合成己酸[J].应用与环境生物学报,2021,27(03):1-14.[doi:10.19675/j.cnki.1006-687x.2020.07002]
 WEI Cuicui,YANG Qingzhuoma,TAO Yong **,et al.Production of caproic acid from glucose by the co-cul ture of Ruminococcaceae bacterium CPB6 and Lactobacillus plantarum RJ25[J].Chinese Journal of Applied & Environmental Biology,2021,27(03):1-14.[doi:10.19675/j.cnki.1006-687x.2020.07002]
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瘤胃菌与乳酸菌共培养利用葡萄糖合成己酸()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
27卷
期数:
2021年03期
页码:
1-14
栏目:
出版日期:
2021-06-25

文章信息/Info

Title:
Production of caproic acid from glucose by the co-cul ture of Ruminococcaceae bacterium CPB6 and Lactobacillus plantarum RJ25
作者:
魏翠翠12 央青卓玛12 陶勇1** 顾文智12 郑德聪12 何晓红1
1中国科学院环境与应用微生物重点实验室&四川省环境微生物重点实验室,中国科学院成都生物研究所 成都 610041
2中国科学院大学 北京 100049
Author(s):
WEI Cuicui12 YANG Qingzhuoma12 TAO Yong 1**GU Wenzhi12 ZHENG Decong12& HE Xiaohong1
1 Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences & Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu 610041, China
2 University of Chinese Academy of Sciences, Beijing 100049, China
关键词:
己酸共培养瘤胃菌CPB6植物乳杆菌RJ25响应面设计
Keywords:
caproic acid co-culture Ruminococcaceae bacterium CPB6 Lactobacillus plantarum RJ25 response surface methodology
DOI:
10.19675/j.cnki.1006-687x.2020.07002
摘要:
己酸因其高附加值而成为生物质资源化领域的一个研究热点。瘤胃菌(Ruminococcaceae bacterium CPB6 )能高效利用乳酸生产己酸,但其利用葡萄糖生产己酸能力较差,增加了发酵成本。采用CPB6与植物乳杆菌(Lactobacillus plantarum RJ25)共培养的发酵方式,经单因素实验确定对共培养产己酸影响显著的3个因素是乙酸钠、葡萄糖、蛋白胨。利用最陡爬坡试验逼近3个因素的最大己酸产量响应区域,进而通过响应面设计及分析建立二次回归模型确定了3个因素的最佳水平:乙酸钠12.37 g/L、葡萄糖52.643 g/L 、蛋白胨30.269 g/L。结果显示,在优化水平下,两种菌共培养时利用葡萄糖生产己酸17.794 g/L ,是CPB6单菌种利用葡萄糖生产己酸(7.726 g/L)的2.3倍,己酸生产效率由21.77%提升至48.37%。本研究表明瘤胃菌与乳酸菌共培养可以显著提高葡萄糖转化为己酸的效率。(图5表5参39)
Abstract:
Caproic acid is gaining increased attention in the field of biomass due to its high value as a chemical feedstock. Ruminococcaceae bacterium CPB6 can efficiently produce caproic acid from lactic acid, but it is less able to produce caproic acid from glucose, which increases the cost of fermentation. In this study, Co-culture of CPB6 and Lactobacillus plantarum RJ25 was carried ou t in the medium containing glucose. Through single factor experiment, the three factors that significantly affected the yield of caproic acid were identified, followed by sodium acetate, glucose, and peptone. The steepest climbing test for the three significant factors was performed to approach the maximum area of caproic ac id production. Finally, response surface methodology (RSM) was employed to optimize the medium for maximum caproic acid production and to establish a quadratic regression model and determine the optimal levels of the three factors: sodium acetate 12.37 g/L, glucose 52.643 g /L, peptone 30.269 g/L. Compared with the yield of 7.726 g/L caproic acid fermented by CPB6 in the medium containing glucose , the final yield of caproic acid by co- culture under the optimum medium could reach 17.794 g/L, which was 2.3 times that by only CPB6. The production efficiency of caproic acid was increased from 21.77% to 48.37%. This study showed that co-culture of CPB6 and L. plantarum RJ25 could significantly improve the conversion efficiency of glucose into caproic acid.

相似文献/References:

[1]魏娜,朱晓宇,陶勇,等.浓香型白酒窖泥中优势菌群的定量PCR分析[J].应用与环境生物学报,2015,21(04):635.[doi:10.3724/SP.J.1145.2015.02021]
 WEI Na,ZHU Xiaoyu,TAO Yong,et al.Detection of dominant prokaryotes in pit mud of Chinese strong-flavor liquor based on qPCR[J].Chinese Journal of Applied & Environmental Biology,2015,21(03):635.[doi:10.3724/SP.J.1145.2015.02021]
[2]郑少睿,朱晓宇,周立祥,等.氯化钠对乳酸合成己酸效能及功能微生物群落的影响[J].应用与环境生物学报,2019,25(06):1441.[doi:10.19675/j.cnki.1006-687x.2019.02018]
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[3]王薪淯,朱晓宇,李海翔,等.[综 述] 乳酸碳链延长技术及其在有机废弃物资源化中的应用研究进展[J].应用与环境生物学报,2020,26(04):827.
 WANG Xinyu,ZHU Xiaoyu?,et al.The biotechnology of lactate chain elongation and its current advances in n-caproate production from organic waste streams[J].Chinese Journal of Applied & Environmental Biology,2020,26(03):827.

备注/Memo

备注/Memo:

收稿日期 Received: 2020-07-02 接受日期 Accepted: 2020-08-03
国家自然科学基金项目(31770090)和中国科学院环境与应用微生物重点实验室开放课题(KLCAS-2017-01)资助
**通讯作者 Corresponding author (E-mail: taoyong@cib.ac.cn)
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更新日期/Last Update: 2020-09-10