|本期目录/Table of Contents|

[1]王鑫 李才武 晋蕾 赵玲玲 邓雯文 李倜 熊跃武 张贵权 李果 何永果 吴代福 张和民 黄炎** 邹立扣**.不同地区圈养大熊猫肠道细菌菌群多样性及组成[J].应用与环境生物学报,2021,27(03):1-10.[doi:10.19675/j.cnki.1006-687x.2020.06072]
 WANG XinLI CaiwuJIN LeiZHAO LinglingDENG WenwenLI TiXIONG YuewuZHANG GuiquanLI GuoHE YongguoWU DaifuZHANG HeminHUANG Yan** &ZOU Likou**.Analysis of gut microbiome diversity and community structure of captive giant pandas in different regions *[J].Chinese Journal of Applied & Environmental Biology,2021,27(03):1-10.[doi:10.19675/j.cnki.1006-687x.2020.06072]
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不同地区圈养大熊猫肠道细菌菌群多样性及组成()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
27卷
期数:
2021年03期
页码:
1-10
栏目:
研究论文
出版日期:
2021-06-25

文章信息/Info

Title:
Analysis of gut microbiome diversity and community structure of captive giant pandas in different regions *
作者:
王鑫1 李才武2 晋蕾1 赵玲玲3 邓雯文2 李倜2 熊跃武2 张贵权2 李果2 何永果2 吴代福 2 张和民2 黄炎2** 邹立扣1**
1四川农业大学资源学院 成都 611130
2大熊猫国家公园珍稀动物保护生物学国家林业和草原局重点实验室(中国大熊猫保护研究中心) 都江堰 611830
3 南京红山森林动物园 南京 210028
Author(s):
WANG Xin1LI Caiwu2JIN Lei1ZHAO Lingling3DENG Wenwen2LI Ti2XIONG Yuewu2ZHANG Guiquan2LI Guo2HE Yongguo2WU Daifu2ZHANG Hemin2HUANG Yan2** &ZOU Likou1**
1 College of Resources, Sichuan Agricultural University, Chengdu 611130, China
2 Key Laboratory of State Forestry and Grassland Administration on Conservation Biology of Rare Animals in the Giant Panda National Park, China Conservation and Research Center for the Giant Panda, Dujiangyan 611830, China
3 Nanjing Hongshan Forest Zoo , Nanjing 210028, China
关键词:
大熊猫高通量测序技术不同地区肠道菌群多样性
Keywords:
giant pandas high -throughput sequencing technology different regions gut microbiome diversity
DOI:
10.19675/j.cnki.1006-687x.2020.06072
摘要:
大熊猫(Ailuropoda melanoleuca)肠道内微生物丰富多样,年龄、生存环境等是影响大熊猫肠道菌群组成的重要因素。为研究国内不同地区的圈养大熊猫肠道细菌组成及多样性,通过高通量测序技术对国内74只圈养大熊猫肠道细菌进行同步测序。结果显示,大熊猫肠道中的细菌,在门分类水平上主要为Proteobacteria(61.95%)和Firmicutes(29.08%),在属分类水平上主要为Escherichia(24.92%)、Pseudomonas(21.60%)、Streptococcus(9.33%)、Clostridium(9.58%)和Sphingobacterium(6.74%)等。大熊猫在适应不同圈舍单位的过程中,肠道核心菌群Escherichia、Pseudomonas、Streptococcus和Clostridium会得以保留但丰度会发生不同程度的改变,并会产生新的特有菌群。不同地区亚成年大熊猫菌群Alpha多样性无显著差异(P > 0.05),但Beta多样性(保定和广州)存在显著差异(P < 0.05),而不同地区(核桃坪和上海)成年大熊猫菌群Alpha多样性存在显著差异(P < 0.05),但Beta多样性无显著差异(P > 0.05)。饲喂同种竹子的圈养大熊猫肠道细菌菌群结构相似。总体而言,地理环境不同可影响圈养成年大熊猫肠道细菌菌群,饲喂不同种类的竹子是导致不同地区圈养大熊猫肠道细菌菌群差异的因素之一。(图5表3参42)
Abstract:
The gut microbiota of giant panda is abundant, and age and living environment are important factors affecting the composition of intestinal microbiota. To investigate the effects of different captive regions, the structure and composition of bacteria of 74 giant pandas from different regions were studied using high-throughput sequencing technology. The result showed that the most dominant phyla in giant pandas consisted of Proteobacteria (61.95%) and Firmicutes (29.08%). At the genus level, the bacteria mainly consisted of Escherichia (24.92%), Pseudomonas (21.60%), Streptococcus (9.33%), Clostridium (9.58%) and Sphingobacterium (6.74%) in giant pandas. When the giant pandas in China Conservation and Research Center for the Giant Panda were transferred into other cities or regions, the core bacteria species, such as Escherichia , Pseudomonas, Streptococcus and Clostridium, were retained, but it appears that the changes in abundance and unique flora have been developed. There was no significant different in sub-adult giant pandas according to Alpha diversity (P >0.05), but t he gut microbiomes of sub-adult giant pandas in Baoding group and Guangzhou group were significantly different (P < 0.05) according to Beta diversity. The community diversity of adult giant pandas in Hetaoping group and Shanghai group was significantly different ( P < 0.05) according to Alpha diversity, while there was no significant difference in all adult giant pandas according to Beta diversity ( P >0.05). Similar gut bacterial community was found in giant pandas eating same kind of bamboo but from widely different regions. Overall, geographical environments probably could affect intestinal microbiota of captive giant pandas. Feeding different kind of bamboo has become one of major factors causing the difference in the intestinal microbial structure of captive giant pandas from different regions.

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备注/Memo

备注/Memo:

收稿日期 Received: 2020-06-30 接受日期 Accepted: 2020-09-04
大熊猫国家公园珍稀动物保护生物学国家林业和草原局重点实验室开放基金项目(中国大熊猫保护研究中心)(KLSFGAGP2020.003)资助
**通讯作者 Corresponding author (E-mail: pandayard@hotmail.com; zoulikou@sicau.edu.cn)
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更新日期/Last Update: 2020-09-10