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[1]申序 陈晓慧 陈荣珠 徐小萍 李晓斐 倪珊珊 张婧 蒋梦琦 林玉玲 赖钟雄**.龙眼LSD1/JmjC基因家族的全基因组鉴定、分子进化及体细胞胚胎发生早期的表达[J].应用与环境生物学报,2021,27(03):1-21.[doi:10.19675/j.cnki.1006-687x.2020.06071]
 SHEN Xu,CHEN Xiaohui,CHEN Rongzhu,et al.Genome-wide identification of longan LSD1/JmjC gene family, molecular evolution, and the expression during early somatic embryogenesis[J].Chinese Journal of Applied & Environmental Biology,2021,27(03):1-21.[doi:10.19675/j.cnki.1006-687x.2020.06071]
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龙眼LSD1/JmjC基因家族的全基因组鉴定、分子进化及体细胞胚胎发生早期的表达()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
27卷
期数:
2021年03期
页码:
1-21
栏目:
研究论文
出版日期:
2021-06-25

文章信息/Info

Title:
Genome-wide identification of longan LSD1/JmjC gene family, molecular evolution, and the expression during early somatic embryogenesis
作者:
申序 陈晓慧 陈荣珠 徐小萍 李晓斐 倪珊珊 张婧 蒋梦琦 林玉玲 赖钟雄**
福建农林大学园艺植物生物工程研究所 福州 350002
Author(s):
SHEN Xu CHEN Xiaohui CHEN Rongzhu XU Xiaoping LI Xiaofei NI Shanshan ZHANG Jing JIANG Mengqi LIN Yuling & LAI Zhongxiong**
Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
关键词:
龙眼组蛋白去甲基化LSD1/JmjC基因家族生物信息学胁迫应答体细胞胚胎发生分子进化
Keywords:
longan histone demethylation DlLSD1/DlJmjC gene family bioinformatics stress response somatic embryogenesis molecular evolution
DOI:
10.19675/j.cnki.1006-687x.2020.06071
摘要:
组蛋白去甲基化修饰是染色质修饰中组蛋白修饰的一种重要方式。赖氨酸特异的去甲基化酶(Lysine-Specific Demethylase1,LSD1)和Jumonji C(JmjC)结构域包含蛋白作为两种组蛋白去甲基化酶,被报道与植物营养生长、生殖发育和疾病抗性有关。然而,组蛋白去甲基化修饰酶LSD1/JmjC在龙眼中的功能仍是不清楚的。使用龙眼“红核子”品种早期体细胞胚胎发生的胚性愈伤组织(embryogenic callus,EC)、不完全胚性紧实结构(incomplete embryogenic compact structures,ICpEC)和球形胚(globular embryos,GE)为材料,通过生物信息学和实时荧光定量(real-time quantitative PCR,qRT-PCR)方法分析DlLSD1/DlJmjC基因在龙眼早期体细胞胚胎发生中的作用。结果显示,龙眼基因组存在4个DlLSD1基因和18个DlJmjC基因,进化树分析显示这些基因被分为LSD1、KDM3/JMJD1、KDM4/JMJD2、KDM5/JARID和JmjC domain only组。全基因组或片段复制事件被确定在DlLSD1/DlJmjC基因家族扩大的进程中发挥重要的作用,而同线性分析和系统发育比较为DlLSD1/DlJmjC基因的进化特征提供更深的认识。蛋白-蛋白互作预测显示,DlJMJ11、DlJMJ12和DlJMJ13蛋白具有较强的相关性,启动子分析也发现,DlLDL1、DlJMJ11、DlJMJ12、DlJMJ14、DlJMJ16-1、DlJMJ17和DlJMJ32等基因存在较多的胁迫和激素响应元件,暗示它们在胁迫和激素调控网络中的重要作用。转录组数据和qRT-PCR分析的表达谱显示:从龙眼EC分化到GE,DlJMJ14和DlJMJ17表达显著逐渐上调,但DlJMJ26和DlLDL1显著逐渐下调;DlJMJ11、DlJMJ12、DlJMJ14、DlJMJ17和DlLDL1均响应水杨酸(salicylic acid,SA)和茉莉酸甲酯(methyl jasmonate,MeJA)调控,而DlJMJ12和DlLDL1也被脱落酸(abscisic acid,ABA)和赤霉素(gibberellin A3,GA3)抑制表达,且DlJMJ11、DlJMJ14、DlJMJ17和DlLDL1均在SA和MeJA处理4 h表达量显著上调;DlJMJ11、DlJMJ12、DlJMJ17和DlLDL1均响应低温调控,它们中的DlJMJ11和DlLDL1响应高温调控,DlJMJ12和DlJMJ17被高温抑制表达。本研究表明相比DlJmjC在进化上存在一定保守性,DlLSD1存在进化上的高度保守,且DlLSD1/DlJmjC可能通过响应激素和非生物胁迫调控来参与龙眼体细胞胚胎发育和发生过程。
Abstract:
Histone demethylation modification is an important way of histone modification in chromatin modification. Lysine-Specific Demethylase1 (LSD1) and Jumonji C (JmjC) domain-containing proteins are two kinds of histone demethylases, which has been reported to be involved in plant vegetative growth, reproductive development and disease resistance. However, the function of histone demethylation modification enzyme LSD1/JmjC in longan were still unclear. We used the embryogenic callus (EC), incomplete compact proembryogenic cultures (ICpEC) and globe embryos (GE) of early somatic embryogenesis (SE) of longan cultivar “Honghezi”, and the bioinformatic methods and real-time quantitative PCR were used to analyze the roles of DlLSD1/DlJmjC genes during early SE in longan. The results show that longan genome existed 4 DlLSD1 and 18 DlJmjC genes, and phylogenetic tree analysis showed these genes were divided into LSD1, KDM3/JMJD1, KDM4/JMJD2, KDM5/JARID and JmjC domain only groups according to their structural and phylogenetic features. Whole genomic duplication (WGD) and segmental duplication events played an important role in the expansion of longan DlLSD1/DlJmjC gene family. However, synteny analysis and phylogenetic comparison provided deep insights into the evolutionary characteristics of DlLSD1/DlJmjC genes in longan. Protein-protein interaction predictions have shown that DlJMJ11, DlJMJ12 and DlJMJ13 proteins have a strong correlation, and promoter analysis also found that DlLDL1, DlJMJ11, DlJMJ12, DlJMJ14, DlJMJ16-1, DlJMJ17 and DlJMJ32 genes exist more stress and hormone response elements, which suggested their important role in stress and hormone regulatory networks. Expression profiles derived from transcriptome data and qRT-PCR analysis exhibited that the expression of DlJMJ14 and DlJMJ17 were significantly gradually upregulated during the differentiation from EC into GE, but DlJMJ26 and DlLDL1 were significantly gradually downregulated; DlJMJ11, DlJMJ12, DlJMJ14, DlJMJ17 and DlLDL1 all responded to the regulation of salicylic acid (SA) and methyl jasmonate (MeJA), while the expression of DlJMJ12 and DlLDL1 was inhibited by abscisic acid (ABA) and gibberellin A3 (GA3), DlJMJ12 also was inhibited by SA and MeJA. Moreover, the expression levels of DlJMJ11, DlJMJ14, DlJMJ17 and DlLDL1 were significantly upregulated at 4 h SA and MeJA treatment; DlJMJ11, DlJMJ12, DlJMJ17 and DlLDL1 all responded to the regulation of low temperature , among them, DlJMJ11 and DlLDL1 both responded to the regulation of high temperature , yet the expression of DlJMJ12 and DlJMJ17 was inhibited by high temperature . This study indicated that DlLSD1 is highly conserved compared to DlJmjC during the evolution process, and DlLSD1/DlJmjC may be involved in longan somatic embryonic development and somatic embryogenesis process by responding to hormone and abiotic stress regulation.

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备注/Memo

备注/Memo:

收稿日期 Received: 2020-06-29 接受日期 Accepted: 2020-08-23
国家自然科学基金项目(31672127,31572088)、福建省自然科学基金(2015?06004)、福建省高原学科建设经费(102/71201801101)和福建农林大学科技创新基金(CXZX2017314,CXZX2017189,CXZX2016118,CXZX2018076,KF2015108)资助 Supported by the National Natural Science Foundation of China (31672127, 31572088), Natural Science Foundation of Fujian Province (2015 J06004), Plateau Discipline Construction Project of Fujian Province (102/71201801101), sci-tech innovation foundation of Fujian Agriculture and Forestry University (CXZX2017314, CXZX2017189, CXZX2016118, CXZX2018076, KF2015108)
**通讯作者 Corresponding author (E-mail: Laizx01@163.com)
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更新日期/Last Update: 2020-09-10