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[1]郭烨# 李绅崇# 王星淇 樊玥 武欢 卢珍红 杨春梅 程春振**.非洲菊CAD基因的克隆及非生物胁迫下的表达[J].应用与环境生物学报,2021,27(03):1-14.[doi:10.19675/j.cnki.1006-687x.2020.06054]
　GUO Ye#,LI Shenchong#,WANG Xingqi,et al.Cloning and expression analysis of CAD genes under different abiotic stresses in gerbera[J].Chinese Journal of Applied & Environmental Biology,2021,27(03):1-14.[doi:10.19675/j.cnki.1006-687x.2020.06054]
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非洲菊CAD基因的克隆及非生物胁迫下的表达()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
27卷
期数:
2021年03期
页码:
1-14
栏目:
研究论文
出版日期:
2021-06-25

文章信息/Info

Title:
Cloning and expression analysis of CAD genes under different abiotic stresses in gerbera
作者:
郭烨1# 李绅崇2# 王星淇1 樊玥1 武欢1 卢珍红2; 3 杨春梅2; 3 程春振1**
1福建农林大学园艺学院/园艺植物生物工程研究所 福州 350002
2云南省农业科学院花卉研究所 昆明 650100
3玉溪云星生物科技有限公司 玉溪 652604
Author(s):
GUO Ye1#;  LI Shenchong2#;  WANG Xingqi1;  FAN Yue1;  WU Huan1;  LU Zhenhong2; 3;  YANG Chunmei2; 3;  CHENG Chunzhen1**
1 College of Horticulture Institute of Horticultural Biotechnology/Fujian Agriculture and Forestry University, Fuzhou 35002, China
2 Flower Research Institute, Yunnan Academy of Agricultural Sciences, Kunming 650205, China
3 Yuxi Yunxing Biotechnology Co., Ltd ., Yuxi, Yunnan 652604, China
关键词:
非洲菊; 肉桂醇脱氢酶; 基因克隆; 非生物胁迫; 基因表达
Keywords:
gerbera;  cinnamyl alcohol dehydrogenase ;  gene cloning;  abiotic stress;  gene expression
DOI:
10.19675/j.cnki.1006-687x.2020.06054
摘要:
为揭示非洲菊肉桂醇脱氢酶基因（GjCAD）的序列特性及其在非生物胁迫下的表达模式，利用反转录PCR（RT-PCR）从非洲菊品种“玲珑”中克隆获得两个GjCADs（GjCAD3和GjCAD5），分析了它们及其编码蛋白序列特性，并利用实时荧光定量PCR（qRT-PCR）技术研究了它们在不同组织器官（根、叶和叶柄）和不同逆境处理（4 ℃低温、PEG模拟干旱、水杨酸（SA）和NaCl处理）下的表达情况。结果显示：GjCAD3和GjCAD5编码序列（CDS）长度分别为1089 bp和1077 bp，它们编码的蛋白均不含信号肽和跨膜结构域，其中GjCAD3属于MDR超家族，为亲水蛋白；GjCAD5属于PLN02514超家族，为疏水蛋白。qRT-PCR结果显示，GjCAD3和GjCAD5均在根中表达量最高，其次为叶柄，叶最低。低温处理下，GjCAD3除在处理24 h后的表达量显著低于对照外，其余时间点均显著高于对照，而GjCAD5的表达则受到显著抑制；干旱处理下，GjCAD3的表达受到显著抑制，而GjCAD5在处理1 h和12 h的表达量显著高于对照，其余时间点与对照相差不大；SA处理下，GjCAD3的表达受到显著抑制，GjCAD5也仅在处理24 h的表达量显著高于对照；盐胁迫处理下，GjCAD3受到显著抑制，而GjCAD5受到显著诱导。上述结果表明GjCAD3和GjCAD5参与非洲菊非生物胁迫应答，但二者表达模式差异较大。
Abstract:
To reveal the sequence characteristics and the expression pattern of cinnamyl alcohol dehydrogenase gene ( GjCAD) under different abiotic stress treatments in gerbera. By u sing reverse transcription PCR (RT-PCR), two GjCADs ( GjCAD3 and GjCAD5) genes were successfully cloned from gerbera jamesonii ‘ Linglong’. Series of bioinformatics software were used for the characterizations of their nucleotide and protein sequences. To show their expression patterns in different tissues and organs (including root, petiole and leaf) and under different abiotic stresses (including 4 ℃ low temperature, PEG mimicked drought, salicylic acid (SA) and NaCl treatments), quantitative real-time quantitative PCR ( qRT-PCR) was used. The coding sequence (CDS) of GjCAD3 and GjCAD5 was 1089 bp and 1077 bp, respectively. Both their encoded proteins are without signal peptide and transmembrane structure. GjCAD3 is a hydrophilic protein belongs to MDR superfamily, while GjCAD5 is a hydrophobic protein belongs to PLN02514 superfamily. qRT-PCR results showed that both GjCAD3 and GjCAD5 expressed the highest in root, followed by petiole and leaf. Under low temperature treatment, the expression of GjCAD3 was significantly induced at all the time-points except at 24 h (significantly lower than control), while the expression of GjCAD5 was significantly inhibited at all time-points. Under drought treatment, GjCAD3 was significantly suppressed, but the GjCAD5 expression at 1 h and 12 h post treatment was significantly induced and at other time-points its expression was similar to control. Under SA treatment, GjCAD3 was significantly inhibited, and GjCAD5 expression was only significantly higher than control at 24 h post treatment. Under salt stress, GjCAD3 was significantly inhibited, while GjCAD5 was significantly induced. Both GjCAD3 and GjCAD5 were found to be widely involved in gerbera abiotic stress responses, but their functions varied a lot.

相似文献/References:

[1]王星淇,孙雪丽,车婧如,等.两个非洲菊4CL基因的克隆及表达[J].应用与环境生物学报,2020,26(02):272.[doi:10.19675/j.cnki.1006-687x.2019.07025]
　WANG Xingqi,SUN Xueli,CHE Jingru,et al.Cloning and expression analysis of two 4CL genes in gerbera[J].Chinese Journal of Applied & Environmental Biology,2020,26(03):272.[doi:10.19675/j.cnki.1006-687x.2019.07025]

备注/Memo

备注/Memo:
收稿日期 Received: 2020-06-18 接受日期 Accepted: 2020-08-12
福建省“2011协同创新中心”中国乌龙茶产业协同创新中心专项（闽教科〔2015〕75号）和福建农林大学茶产业链科技创新与服务体系建设项目（2020-01）资助
**通讯作者 Corresponding author (E-mail: ynxtea@126.com; zhaofeng0591@qq.com)
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更新日期/Last Update: 2020-09-10