|本期目录/Table of Contents|

[1]谷梦雅 王鹏杰 金珊 陈思肜 郑知临 赵峰** 叶乃兴**.不同强度红光对茶树苯丙烷类代谢影响的转录组分析[J].应用与环境生物学报,2021,27(03):1-14.[doi:10.19675/j.cnki.1006-687x.2020.06047]
 GU MengyaWANG PengjieJIN ShanCHEN SirongZHENG ZhilinZHAO Feng**&YE Naixing**.Effects of different red LED light intensities on phenylpropanoid metabolism of tea plants based on transcriptomics[J].Chinese Journal of Applied & Environmental Biology,2021,27(03):1-14.[doi:10.19675/j.cnki.1006-687x.2020.06047]
点击复制

不同强度红光对茶树苯丙烷类代谢影响的转录组分析()
分享到:

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
27卷
期数:
2021年03期
页码:
1-14
栏目:
出版日期:
2021-06-25

文章信息/Info

Title:
Effects of different red LED light intensities on phenylpropanoid metabolism of tea plants based on transcriptomics
作者:
谷梦雅1 王鹏杰1 金珊1 陈思肜1 郑知临1 赵峰2** 叶乃兴1**
1福建农林大学园艺学院/茶学福建省高校重点实验室 福州 350002
2福建中医药大学药学院 福州 350122
Author(s):
GU Mengya1WANG Pengjie1JIN Shan1CHEN Sirong1ZHENG Zhilin1ZHAO Feng2**&YE Naixing1**
1College of HorticultureFujian Agriculture and Forestry UniversityKey Laboratory of Tea Science at Universities,Fuzhou 350002 China
2College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China
关键词:
茶树红光转录组差异基因苯丙烷类代谢
Keywords:
Camellia sinensis red light transcriptome differentially expressed genes phenylpropanoid metabolism
DOI:
10.19675/j.cnki.1006-687x.2020.06047
摘要:
为探究不同强度红光对茶树苯丙烷类代谢的影响,通过高通量测序技术对不同强度红光处理的福建水仙茶树新梢进行转录组测序,其中以白色LED灯(100 μmol m -2 s-1)为基础光照,补充3个不同强度红光即120 μmol m -2 s-1、240 μmol m -2 s-1、480 μmol m -2 s-1。结果显示:补充3个不同强度红光与对照相比分别检测到1693个(上调408和下调1285)、879个(上调135和下调744)和6个(上调3和下调3)差异表达基因。利用GO和KEGG数据库,对差异基因进行代谢通路富集分析,发现补充120 μmol m -2 s -1和240 μmol m -2 s-1红光茶树的差异基因显著富集在苯丙烷类生物合成、氨基糖和核苷酸糖代谢等代谢通路中。利用实时荧光定量PCR验证了随机挑选的9个差异基因(其中4-香豆酸辅酶A连接酶(TEA034012)、乙醛脱氢酶(TEA033457)、肉桂醇脱氢酶(TEA025404)和咖啡酰辅酶A-O-甲基转移酶(TEA030958)是苯丙烷类代谢途径的基因)在不同强度红光处理的相对表达量,结果与转录组测序结果的变化趋势一致。本研究表明4CL、CAD和COMT可能是红光调控下苯丙烷类生物合成代谢途径中的关键基因,结果可为茶树次生代谢机制的调控研究提供借鉴。(图7表2参32)
Abstract:
To study the effect of different intensity red light on phenylpropanoid metabolism of tea plants, this study carried out transcriptome sequencing of the new shoots of Fujian daffodil tea plants treated with different intensity red light by high -throughput sequencing technology, in which white light (100 μmol m -2 s-1) basic light was used as control to supplement three different intensity red light(120 μmol m -2 s-1, 240 μmol m -2 s -1, 480 μmol m -2 s -1). Daffodil tea trees treated with different intensity red light were subjected to new slightly transcriptome sequencing by high -throughput sequencing. The results showed that detected 1693 (up-regulation 408 and down-regulation 1285), 879 (up-regulation 135 and down-regulation 744) and 6 (up-regulation 3 and down-regulation 3) differentially expressed genes, respectively. Access to GO and KEGG database, the metabolic pathway enrichment analysis of differential genes was carried out, and it was found that the differential genes of 120 μmol m -2 s-1 and 240 μmol m -2 s-1 red light tea plants were significantly enriched in metabolic pathways such as phenylpropanoid biosynthesis, amino sugar and nucleotide sugar metabolism. RT -qPCR was used to verify the relative expression of randomly selected 9 differential genes in different intensity red light processing, which of them are genes of phenylpropanoid metabolism pathway, such as 4 -coumaroyl CoA ligase (TEA034012), aldehyde dehydrogenase (TEA033457), cinnamyl-alcohol dehydrogenase?(TEA025404) and caffeoyl-CoA O-methyltransferase (TEA030958). A nd the results were consistent with the trend of transcriptome sequencing results. This study suggests that 4CL, CAD and COMT may be the key genes in the phenylpropanoid biosynthesis pathway regulated by red light. This study provides a reference for the study of the regulation of secondary metabolism mechanism in tea plants by different intensity red light.

相似文献/References:

[1]黄安平,韩宝瑜,包小村.茶刺蛾危害后茶树挥发性有机化合物释放变化[J].应用与环境生物学报,2011,17(06):819.[doi:10.3724/SP.J.1145.2011.00819]
 HUANG Anping,HAN Baoyu,BAO Xiaocun.Change in Volatile Organic Compounds from Camellia sinensis (L.) O. Kuntze Damaged by Iragoides fasciata Moore (Lepidoptera: Eucleidae)[J].Chinese Journal of Applied & Environmental Biology,2011,17(03):819.[doi:10.3724/SP.J.1145.2011.00819]
[2]王海斌** 叶江华 陈晓婷 贾小丽 孔祥海.连作茶树根际土壤酸度对土壤微生物的影响[J].应用与环境生物学报,2016,22(03):480.[doi:10.3724/SP.J.1145.2015.09019]
 WANG Haibin**,YE Jianghua,CHEN Xiaoting,et al.Effect on soil microbes of the rhizospheric soil acidity of tea tree continuous cropping*[J].Chinese Journal of Applied & Environmental Biology,2016,22(03):480.[doi:10.3724/SP.J.1145.2015.09019]
[3]孙平,章国营,向萍,等.茶树中莽草酸途径DHD/SDH基因的表达调控[J].应用与环境生物学报,2018,24(02):322.[doi:10.19675/j.cnki.1006-687x.2017.05014]
 SUN Ping,ZHANG Guoying,XIANG Ping,et al.Expression and regulation of the shikimic acid pathway gene DHD/SDH in tea plant (Camellia sinensis)[J].Chinese Journal of Applied & Environmental Biology,2018,24(03):322.[doi:10.19675/j.cnki.1006-687x.2017.05014]
[4]王海斌,陈晓婷,丁力,等.不同树龄茶树根际土壤细菌多样性的T-RFLP分析[J].应用与环境生物学报,2018,24(04):775.[doi:10.19675/j.cnki.1006-687x.2017.10003]
 WANG Haibin,**,CHEN Xiaoting,et al.Using T-RFLP technology to analyze bacterial diversity in the rhizospheric soils of tea tree at different ages[J].Chinese Journal of Applied & Environmental Biology,2018,24(03):775.[doi:10.19675/j.cnki.1006-687x.2017.10003]
[5]郭玉琼,黄道斌,常笑君,等.铁观音茶树体胚发生及其内源激素变化[J].应用与环境生物学报,2018,24(04):824.[doi:10.19675/j.cnki.1006-687x.2017.12027]
 GUO Yuqiong,HUANG Daobin,CHANG Xiaojun,et al.Somatic embryogenesis and the changes of endogenous hormones in Camellia sinensis ‘Tieguanyin’[J].Chinese Journal of Applied & Environmental Biology,2018,24(03):824.[doi:10.19675/j.cnki.1006-687x.2017.12027]
[6]郭玉琼,王仲,朱晨,等.茶树CSD1基因及其启动子克隆与低温胁迫下的表达[J].应用与环境生物学报,2018,24(05):1122.[doi:10.19675/j.cnki.1006-687x.2018.02021]
 GUO Yuqiong,WANG Zhong,ZHU Chen,et al.Cloning and expression of the copper/zinc-superoxide dismutase 1 gene and its promoter under low temperature stress in Camellia sinensis[J].Chinese Journal of Applied & Environmental Biology,2018,24(03):1122.[doi:10.19675/j.cnki.1006-687x.2018.02021]
[7]王海斌,陈晓婷,丁力,等.福建省安溪县茶园土壤酸化对茶树产量及品质的影响[J].应用与环境生物学报,2018,24(06):1398.[doi:10.19675/j.cnki.1006-687x.2017.12008]
 WANG Haibin,et al..Effect of soil acidification on yield and quality of tea tree in tea plantations from Anxi county, Fujian Province[J].Chinese Journal of Applied & Environmental Biology,2018,24(03):1398.[doi:10.19675/j.cnki.1006-687x.2017.12008]
[8]岳川,曹红利,王赞,等.茶树RING-finger型E3泛素连接酶基因CsSDIR的克隆与表达[J].应用与环境生物学报,2018,24(06):1375.[doi:10.19675/j.cnki.1006-687x.2018.09010]
 YUE Chuan,et al..Cloning and expression of RING-finger E3 ubiquitin ligase CsSDIR1 gene in tea plant (Camellia sinensis)[J].Chinese Journal of Applied & Environmental Biology,2018,24(03):1375.[doi:10.19675/j.cnki.1006-687x.2018.09010]
[9]郑世仲,江胜滔,陈美霞,等.茶树Ankyrin基因启动子的克隆及其5′UTR内含子功能[J].应用与环境生物学报,2019,25(06):1381.[doi:10.19675/j.cnki.1006-687x.2019.05011]
 ZHENG Shizhong,JIANG Shengtao,et al.Isolation of the Ankyrin gene promoter from tea plant (Camellia sinensis L.) and a subsequent analysis of the function of its 5′UTR intron[J].Chinese Journal of Applied & Environmental Biology,2019,25(03):1381.[doi:10.19675/j.cnki.1006-687x.2019.05011]
[10]项丽慧,陈林,余文权,等.茶树GH1基因家族鉴定及其在茶鲜叶萎凋过程的表达[J].应用与环境生物学报,2020,26(04):878.
 XIANG Lihui,CHEN Lin,YU Wenquan & ZHANG Yinggen.Identification of the GH1 gene family in Camellia sinensis and expression analysis during the withering process of fresh tea leaves[J].Chinese Journal of Applied & Environmental Biology,2020,26(03):878.

备注/Memo

备注/Memo:
收稿日期 Received: 2020-06-18 接受日期 Accepted: 2020-08-12
福建省“2011协同创新中心”中国乌龙茶产业协同创新中心专项(闽教科〔2015〕75号)和福建农林大学茶产业链科技创新与服务体系建设项目(2020-01)资助
**通讯作者 Corresponding author (E-mail: ynxtea@126.com; zhaofeng0591@qq.com)
点击摘要页题目后的“PDF”可下载阅读全文;本文为已录用的作者修定稿,尚未经编辑全面修改。
引用本文请注明出处本刊;发表刊期和页码将以正式出版时的安排为准,但DOI确定不变。
更新日期/Last Update: 2020-09-10