|本期目录/Table of Contents|

[1]潘金程 李艳冰 李晶** 马艳玲**.铜绿假单胞菌降解烃类污染物的泛基因组分析[J].应用与环境生物学报,2021,27(03):1-12.[doi:10.19675/j.cnki.1006-687x.2020.06046]
 PAN Jincheng,LI Yanbing,LI Jing** & MA Yanling **.Functional pan-genome analyses of hydrocarbon biodegradation by diverse Pseudomonas aeruginosa[J].Chinese Journal of Applied & Environmental Biology,2021,27(03):1-12.[doi:10.19675/j.cnki.1006-687x.2020.06046]
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铜绿假单胞菌降解烃类污染物的泛基因组分析()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
27卷
期数:
2021年03期
页码:
1-12
栏目:
研究论文
出版日期:
2021-06-25

文章信息/Info

Title:
Functional pan-genome analyses of hydrocarbon biodegradation by diverse Pseudomonas aeruginosa
作者:
潘金程1 李艳冰1 李晶2** 马艳玲1**
1西北大学生命科学学院 西安 710069
2西安瑞捷生物科技有限公司 西安 710065
Author(s):
PAN Jincheng1 LI Yanbing1 LI Jing2** & MA Yanling 1**
1 College of Life Science, Northwest University, Xian 710069, China
2 Xian Rege Biotechnology Co., Ltd , Xian 710065, China
关键词:
铜绿假单胞菌泛基因组核心基因独特基因生物降解
Keywords:
Pseudomonas aeruginosa pan-genome core genome unique genome biodegradation
DOI:
10.19675/j.cnki.1006-687x.2020.06046
摘要:
铜绿假单胞菌是一种常见的烃类降解菌,在环境污染治理中具有广阔的应用前景。本文利用泛基因组对10株铜绿假单胞菌进行分析以深入了解其泛基因组特征、生物降解潜力及芳香族化合物降解中心途径。结果显示,铜绿假单胞菌的核心基因组大约包含4 923个基因,占泛基因组的56.2%。泛基因组分析表明其泛基因组即将闭合,说明分析的铜绿假单胞菌通过HGT获得新基因的能力较弱。核心基因组包含了大量ABC转运蛋白和双组分调节系统相关基因,氨基酸代谢(合成和降解)、鞭毛组装和生物膜相关基因也是核心基因组的一部分。核心基因的KEGG注释表明,铜绿假单胞菌主要通过4条中心途径来降解芳香族化合物:β-酮己二酸酯、高儿茶酸、尿黑酸和龙胆酸途径,同时还具有相对完整的烷烃羟化酶系统。根据GC含量及侧翼转座酶和整合酶可以得出结论,铜绿假单胞菌可通过HGT获得芳香族化合物降解相关因。本文对10株菌的泛基因组分析表明:铜绿假单胞菌虽然通过横向转移获得新基因的频率较低,但是已拥有完整的烃类降解系统;该分析结果有助于了解铜绿假单胞菌群体的基因组成及基因组分特征,以期对其生物修复潜力及降解机制有更进一步的认识。(图5表2 参52)
Abstract:
As a versatile degrader, Pseudomonas aeruginosa has a promising application in the removal of both saturated aliphatic and aromatic hydrocarbons. To well present bacterial genomic characteristics, the functional genomic analysis among 10 P. aeruginosa strains was employed to interpret the core genome and pan-genome, potential of hydrocarbon biodegradation and central pathway of aromatic compound degradation. Most of homologous genes constituted the core genome that contained 4 923 genes accounting for 56.2% of the pan-genome, with a tiny portion of unique types of genomic evidence in those strains. The open pan-genome was about to close momentarily with its growing number of genomes, implying that the ability to exchange genetic information with the external condition by HGT was feeble among the selected strains of P. aeruginosa. The most abundant genes within the core genome were those that encode nutrient transporters and two-component system. Genes that encode amino acid metabolism (synthesis and degradation), biofilm formation and flagella assembly?were all part of the core genome. Moreover, the KEGG annotation of the core genes demonstrated that β -ketoadipate, homoprotocatechuate, homogentisate and gentisate pathway was the main catabolism process of aromatic compounds in the P. aeruginosa strains, respectively, which possessed concurrently a relatively complete alkane hydroxylase system. Based on the GC content, flanked transposase and integrase coding genes, it could be concluded that the aromatic compounds degradation gene clusters in P. aeruginosa could be obtained through horizontal gene transfer. The functional pan-genome analysis of those 10 strains showed that the frequency of HGT in P. aeruginosa to obtain new genes from the outside world was relatively weak, and had a complete system to degrade hydrocarbon. The results would be beneficial for better understanding the genomic composition and characteristics, as well as provide useful information for potential and mechanism of degradation of P. aeruginosa.

相似文献/References:

[1]张翠坤,杨白雪,常冬妹,等.铜绿假单胞菌D1产鼠李糖脂及降解原油效果[J].应用与环境生物学报,2016,22(22卷04):697.[doi:10.3724/SP.J.1145.2015.11033]
 ZHANG Cuikun,YANG Baixue,CHANG Dongmei & YANG Hongjiang **.Pseudomonas aeruginosa D1: rhamnolipid production and crude oil degradation*[J].Chinese Journal of Applied & Environmental Biology,2016,22(03):697.[doi:10.3724/SP.J.1145.2015.11033]
[2]方源 谢柏盛 黄婷 郑鑫 许子牧** 汪家权**.34株假单胞菌的泛基因组分析[J].应用与环境生物学报,2021,27(01):1.[doi:10.19675/j.cnki.1006-687x.2020.03048]
 Fang Yuan,Xie Baisheng,Zheng Xin,et al.Pan-genomic characteristic of 34 Pseudomonas strains[J].Chinese Journal of Applied & Environmental Biology,2021,27(03):1.[doi:10.19675/j.cnki.1006-687x.2020.03048]

备注/Memo

备注/Memo:

收稿日期 Received: 2020-06-18 接受日期 Accepted: 2020-09-05
国家自然科学基金青年项目(31000069)和陕西省重点研发计划重点项目(2018ZDXM-NY-065)资助
**通讯作者 Corresponding author (E-mail: 30687147@qq.com; mayanling@nwu.edu.cn)

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更新日期/Last Update: 2020-09-10