[1]梅超 刘玉卫 孙蕾 王慧杰 董雪 李欣 冯瑞云**.马铃薯AS2基因家族的鉴定与逆境胁迫表达[J].应用与环境生物学报,2021,27(03):1-15.[doi:10.19675/j.cnki.1006-687x.2020.06036]
MEI Chao,LIU Yuwei,SUN Lei,et al.Identification and expression analysis of the AS2 Gene Family Under Abiotic stress in Solanum tuberosum L.*[J].Chinese Journal of Applied & Environmental Biology,2021,27(03):1-15.[doi:10.19675/j.cnki.1006-687x.2020.06036]
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马铃薯AS2基因家族的鉴定与逆境胁迫表达()
MEI Chao,LIU Yuwei,SUN Lei,et al.Identification and expression analysis of the AS2 Gene Family Under Abiotic stress in Solanum tuberosum L.*[J].Chinese Journal of Applied & Environmental Biology,2021,27(03):1-15.[doi:10.19675/j.cnki.1006-687x.2020.06036]
《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]
- 卷:
- 27卷
- 期数:
- 2021年03期
- 页码:
- 1-15
- 栏目:
- 研究论文
- 出版日期:
- 2021-06-25
文章信息/Info
- Title:
- Identification and expression analysis of the AS2 Gene Family Under Abiotic stress in Solanum tuberosum L.*
- Keywords:
- Solanum tuberosum; AS2 gene family; system development; abiotic stress; expression profile
- 摘要:
- 高盐、干旱、高温、低温等非生物胁迫是造成马铃薯减产的重要因素，鉴定和探究马铃薯逆境胁迫应答相关基因对马铃薯生产具有重要意义。ASYMMETRIC LEAVES2（AS2）基因家族是一类广泛存在于植物中的转录因子家族，在植物生长发育和抵御逆境胁迫过程中发挥重要作用。本研究通过生物信息学系统分析，鉴定得到43个马铃薯AS2基因家族成员。根据保守结构域的差异将AS2基因家族分为ClassⅠ、ClassⅡ两类，其中ClassⅠ分为Ⅰa、Ⅰb、Ⅰc、Ⅰd、Ⅰe五个亚类，ClassⅡ分为Ⅱa、Ⅱb两个亚类。马铃薯AS2蛋白主要以α-螺旋和不规则卷曲两种二级结构组成，定位在细胞核中。通过对马铃薯植株进行高盐、干旱、低温和高温胁迫处理，并对叶片和根中部分AS2基因家族成员的表达量进行分析，发现ClassⅠ类和ClassⅡ类中大部分成员均参与逆境胁迫应答，但在不同逆境胁迫下的响应程度存在差异。高盐胁迫下，叶片中StAS2-7和根中StAS2-27表达量分别是对照的10倍；干旱胁迫下，除StAS2-4外的其余成员在叶片和根部表达量明显上调；高温胁迫下，叶片中StAS2-15表达量下调，其余成员表达上调；低温胁迫下，大部分StAS2成员表达量变化上调。本研究表明马铃薯AS2基因家族成员在系统发育过程中具有保守性与物种特异性，可以通过响应逆境胁迫维持马铃薯体内的正常代谢，增强马铃薯抵御非生物胁迫的能力。（图4 表3 参42）
- Abstract:
- Potato ( Solanum tuberosum L.) is an important crop worldwide. Abiotic stresses, like salt, drought, heat, and cold, inhibit potato growth and development and reduce yield. Therefore, identifying and understanding the genes involved in potato stress tolerance are important for molecular breeding. The transcription factor gene family, ASYMMETRIC LEAVES2 (AS2), is widely found in plants and plays a key role in regulating growth, development, and stress resistance. This study aimed to uncover the AS2 gene family profile, secondary structure, and expression patterns under abiotic stress, and to provide a theoretical basis for the function of the potato AS2 gene family (StAS2) during abiotic stress responses. The StAS2 genes during abiotic stress were identified and classified, their phylogenetic relationships were analyzed, and the expression profiles were measured. The AS2 genes were identified by BLAST software in the potato genome database for the previously reported Arabidopsis AS2 genes. The plants were treated with high-salt, drought, low-temperature, and high-temperature stressors, and the expression levels of the AS2 gene family members in the leaves and roots were analyzed by real-time fluorescent quantitative polymerase chain reaction ( qRT-PCR). Bioinformatic analysis identified 43 StAS2 genes in the potato genome. Based on the differences in the conserved domains, the StAS2 gene family was categorized into ClassI and ClassII. ClassI was classified into five subclasses ( Ia, Ib, Ic, Id, and Ie), and ClassII was classified into IIa and IIb. The potato AS2 protein is composed of two secondary structures, α-helix and random coil, located in the nucleus. Furthermore, the expression patterns of the StAS2 genes in response to abiotic stress were analyzed by qRT-PCR. Most members of class I and class II were involved in the stress response, but the degree of response changed with different stressors, indicating variable regulatory mechanisms. Under salt stress, the expression levels of StAS2-7 in leaves and StAS2-27 in roots were up-regulated ten- foldand compared with the control, respectively. Under drought stress, all StAS2 members were significantly up-regulated in leaves and roots except StAS2-4. Under high temperature stress, StAS2-15 was down-regulated in leaves but the others were up-regulated both in leaves and roots. Moreover, most of StAS2 members were up-regulated after cold stress. Taken together, these results indicate that the members of StAS2 gene family were conserved and species-specific by phylogenetic analysis, demonstrating StAS2 genes play an important role in abiotic stress tolerance through maintaining the normal metabolism of potato.
相似文献/References:
[1]叶彦,缪树华.长期继代培养马铃薯愈伤组织的植株再生[J].应用与环境生物学报,1995,1(01):26.
Ye Yan,Miao Shuhua.PLANT REGENERATION FROM LONG-TERM SUBCULTURED CALLI OF POTATO[J].Chinese Journal of Applied & Environmental Biology,1995,1(03):26.
[2]唐巍,杨映根,郭仲琛,等.马铃薯微型薯诱导的激素调节[J].应用与环境生物学报,1996,2(04):347.
Tang Wei,Yang Yinggen,Guo Zhongchen,et al.HORMONAL CONTROL ON THE FORMATION OF MICROTUBERS OF POTATO[J].Chinese Journal of Applied & Environmental Biology,1996,2(03):347.
[3]梅超,刘玉卫,孙蕾,等.马铃薯AS2基因家族的鉴定与逆境胁迫表达[J].应用与环境生物学报,2020,26(06):1498.[doi:DOI: 10.19675/j.cnki.1006-687x.2020.06036]
MEI Chao,LIU Yuwei,SUN Lei,et al.Identification and expression analysis of the AS2 gene family under abiotic stress in Solanum tuberosum L.[J].Chinese Journal of Applied & Environmental Biology,2020,26(03):1498.[doi:DOI: 10.19675/j.cnki.1006-687x.2020.06036]
备注/Memo
- 备注/Memo:
-
收稿日期 Received: 2020-06-15 接受日期 Accepted: 2020-08-05
山西省重点研发计划项目（201903D221076）、山西省农业科学院应用基础研究计划（YGC2019FZ1）和山西省农业科学院作物科学研究所博士基金（ZB1901）资助
**通讯作者 Corresponding author (E-mail: Fengruiyun1920@163.com)
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更新日期/Last Update:
2020-09-10