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[1]郝鹏 张喜庆 高云航** 刘继军 陆伟 韩永胜 郝剑刚 成海建.抗生素对复合菌系降解17β-雌二醇的影响[J].应用与环境生物学报,2021,27(03):1-12.[doi:10.19675/j.cnki.1006-687x.2020.06025]
　HAO Peng,ZHANG Xiqing,GAO Yunhang **,et al.Effect of Antibiotics on Degradation of 17-estradiol by Compound Bacteria[J].Chinese Journal of Applied & Environmental Biology,2021,27(03):1-12.[doi:10.19675/j.cnki.1006-687x.2020.06025]
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抗生素对复合菌系降解17β-雌二醇的影响()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
27卷
期数:
2021年03期
页码:
1-12
栏目:
出版日期:
2021-06-25

文章信息/Info

Title:
Effect of Antibiotics on Degradation of 17-estradiol by Compound Bacteria
作者:
郝鹏1 张喜庆1 高云航1** 刘继军2 陆伟3 韩永胜4 郝剑刚5 成海建6
1吉林农业大学动物科学技术学院,长春 130118
2中国农业大学动物科学技术学院,北京 100083
3长春市朝阳区动物疫病控制中心,长春 130012
4国家肉牛牦牛产业技术体系齐齐哈尔综合实验站,黑龙江齐齐哈尔
5国家肉牛牦牛产业技术体系乌拉盖综合实验站,内蒙古锡林浩特
6国家肉牛牦牛产业技术体系济南综合实验站,山东济南
Author(s):
HAO Peng1;  ZHANG Xiqing 1;  GAO Yunhang 1**;  LIU Jijun2;  LU Wei3;  HAN Yongsheng4;  HAO Jiangang5 & CHENG Haijian 6
1College of Animal Science and Technology, Jilin Agricultural University , Changchun 130118, China
2College of Animal Science and Technology, China Agricultural University, Beijing 100083, China
3Animal Disease Control Center of Chaoyang District, Changchun City, Changchun 130012, China
4Qiqihar Experiment Station,National Beef Cattle Industrial Technology System , Qiqihar, Heilongjiang province, China
5Wulagai Experiment Station,National Beef Cattle Industrial Technology System , Xilin Haote, Inner Mongolia, China
6Jinan Experiment Station,National Beef Cattle Industrial Technology System , Jinan, Shandong province, China
关键词:
17β-雌二醇; 降解; 复合菌系; 抗生素
Keywords:
17-estradiol;  complex microbial system;  antibiotic;  degradation
DOI:
10.19675/j.cnki.1006-687x.2020.06025
摘要:
为丰富17β-雌二醇（E2）优势降解菌种，采用富集驯化法从养殖场粪污中筛选到6株对E2降解率在25%以上的功能性菌株，经菌株鉴定及牛津杯抑菌实验后，构建复合菌系并选择最优组。通过分析温度、pH、盐度、底物浓度等对复合菌系降解E2的影响，确定最佳降解条件。针对养殖粪污抗生素残留问题，向降解培养基中添加6种常用抗生素，研究不同抗生素对复合菌系降解E2效率的影响。结果表明，复合菌系A对E2降解效果显著。其最佳降解条件为：温度30 ℃，初始pH 7、底物浓度20 mg/L，在此条件下降解培养5 d，E2降解率可达91.3%。抗生素浓度为1 mg/L时，磺胺嘧啶（SD）显著促进E2降解（P < 0.05），土霉素（OTC）和磺胺甲恶唑（SMZ）显著抑制E2降解（P < 0.05），金霉素（CTC）、泰乐菌素（TYL）和强力霉素（DC）对E2降解没有显著影响；当抗生素浓度为10 mg/L时，除SD外所有抗生素均显著抑制E2降解（P < 0.05），其中DC极显著抑制降解，降解率降低9.6%，但复合菌系A对E2降解率仍达80%以上。本研究构建的复合菌系A可有效提高雌激素E2的降解效率，且受抗生素影响较低，对微生物法降解E2具有一定借鉴意义。
Abstract:
In order to enrich 17 β -estradiol (E2) dominant degradation strains, this study aimed to construct the optimal mixed strains from some functional bacteria. There are six strains from the manure field were selected after bacteria identification and Oxford cup bacteriostases experiment, and E2 degradation rate of all the six was above 25%. The optimal degradation conditions of mixed strains were determined by analyzing the effects of temperature, pH value, salinity and substrate concentration on the degradation of E2. Due to the residual of antibiotics in manure, six kinds of commonly used antibiotics were added to the medium to study the effect of different antibiotics on the degradation efficiency of E2 in mixed bacteria. The result showed that mixed strains A has a significant effect on degrading E2. The optimal conditions for degradation were temperature 30, initial pH7, substrate concentration 20mg/L. The degradation rate of E2 was up to 91.3% cultured under above condition for 5 days. When antibiotic concentration was 1 mg/L, sulfadiazine (SD) significantly promoted the degradation of E2 ( P < 0.05), but oxytetracycline (OTC) and sulfamethoxazole (SMZ) inhibited E2 degradation ( P < 0.05). Aureomycin (CTC), Tylosin (TYL) and doxycycline (DC) had no significant influence on E2 degradation. When antibiotic concentration was 10 mg/L, all antibiotics except SD significantly inhibited the degradation of E2 ( P < 0.05), and OTC very significantly inhibited the degradation, whose rate was reduced by 9 .6%, but the degradation rate of mixed strain A to E2 is still over 80%. The mixed strains A constructed in this study can effectively improve the degradation efficiency of E2 and is less affected by antibiotics, which has certain reference significance for the degradation of E2 by microbial method.

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备注/Memo

备注/Memo:

收稿日期 Received: 2020-06-11 接受日期 Accepted: 2020-09-08-23
国家重点研发计划专项（2016YFD0501400，2018YFD0501800）、国家现代农业产业技术体系建设专项（CARS-37）、吉林省科技发展计划项目（20190201296JC）和内蒙古自治区重点研发计划项目（2017BY082）资助
**通讯作者 Corresponding author (E-mail: gaoyunhang@163.com)
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更新日期/Last Update: 2020-09-10