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[1]陈潇敏 王鹏杰 王淑燕 杨如兴 孙君 郭永春 陈雪津 赵峰** 叶乃兴**.基于转录组的蕉城苦茶苦茶碱合成相关基因的挖掘[J].应用与环境生物学报,2021,27(03):1-12.[doi:10.19675/j.cnki.1006-687x.2020.05041]
　CHEN XiaominWANG PengjieWANG ShuyanYANG RuxingSUN JunGUO YongchunCHEN XuejinZHAO Feng** & YE Naixing**.Excavation of genes involved in theacrine bio synthesis of Jiaochengkucha based on transcriptome[J].Chinese Journal of Applied & Environmental Biology,2021,27(03):1-12.[doi:10.19675/j.cnki.1006-687x.2020.05041]
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基于转录组的蕉城苦茶苦茶碱合成相关基因的挖掘()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
27卷
期数:
2021年03期
页码:
1-12
栏目:
研究论文
出版日期:
2021-06-25

文章信息/Info

Title:
Excavation of genes involved in theacrine bio synthesis of Jiaochengkucha based on transcriptome
作者:
陈潇敏1 王鹏杰1 王淑燕1 杨如兴2 孙君2 郭永春1 陈雪津1 赵峰3** 叶乃兴1**
1福建农林大学园艺学院,茶学福建省高校重点实验室 福州 350002
2福建省农业科学院茶叶研究所 福州 35 0012
3福建中医药大学药学院 福州 350122
Author(s):
CHEN Xiaomin1WANG Pengjie1WANG Shuyan1YANG Ruxing2SUN Jun2GUO Yongchun1CHEN Xuejin1ZHAO Feng3** & YE Naixing1**
1 College of Horticulture, Fujian Agriculture and Forestry University, Key Laboratory of Tea Science at Universities,Fuzhou 350002, China
2Tea Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350012, China
3College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou350122, China
关键词:
茶树; 蕉城苦茶; 苦茶碱; 咖啡碱; 转录组分析
Keywords:
tea plant;  Jiaochengkucha;  theacrine; caffeine;  transcriptome analysis
DOI:
10.19675/j.cnki.1006-687x.2020.05041
摘要:
为探究茶树苦茶碱积累的分子机制，以福建省发现的首个含有苦茶碱的茶树种质资源蕉城苦茶为供试材料，并以福鼎大白茶为参照组，采用超高效液相色谱-四级杆飞行时间质谱测定其生物碱组分并进行转录组测序分析。结果表明：蕉城苦茶的生物碱含量显著高于福鼎大白茶，且以苦茶碱为主（苦茶碱含量：25.0 ± 0.06 g/kg），而福鼎大白茶未检出苦茶碱。基于转录组数据筛选获得48条苦茶碱合成相关的基因序列，KEGG通路富集分析将其聚焦至4个代谢通路，分别是鸟嘌呤降解途径、腺嘌呤降解途径、咖啡碱合成途径以及咖啡碱代谢途径。进一步分析发现，在蕉城苦茶中，尿酸氧化酶UOX（TEA012458）表达量相对较高，咖啡碱合成酶TCS（TEA030024）表达量相对较低。总之，鸟嘌呤和腺嘌呤降解途径、咖啡碱代谢相关途径是影响茶树苦茶碱合成的重要代谢通路，其中尿酸氧化酶UOX（TEA012458）和咖啡碱合成酶TCS（TEA030024）可能为调控苦茶碱合成的候选基因，该结果为探明茶树苦茶碱的生成机制提供了参考依据。（图5表4参32）
Abstract:
In order t o explore the molecular mechanism of the accumulation of theacrine in Fujian bitter tea Jiaochengkucha, the first tea germplasm resource containing theacrine in Fujian province, was selected as the object in this study, and using the Fudingdabaicha as the control cultivar. The alkaloid component was measured by HPLC and UFLC-Q-TOF-MS/MS and transcriptome of them. The result showed that: the content of alkaloid in Jiaochengkucha is higher than Fudingdabaicha, the highest content is theacrine. (theacrine content: 25.0 ± 0.06 g/kg). While, Fudingdabaicha undetected theacrine. Based on RNA- Seq, 48 unigenes were identified to be involved in theacrine biosynthetic pathway. KEGG pathway analysis showed that differentially expression genes annotated to four metabolic pathways, including guanine degradation, ad enine degradation, caffeine synthesis and caffeine degradation. Further analysis showed that urate oxidase UOX（TEA012458） is down regulated, while tea caffeine synthase TCS（TEA030024） is up regulated in Jiaochengkucha. In short, the degradation pathways of guanine and adenine,and the pathways related caffeine metabolism were the important metabolic pathways th at affect the synthesis of theacrine in Jiaochengkucha. Among them, urate oxidase UOX（TEA012458）and tea caffeine synthase TCS（TEA030024）may be candidate genes regulating the synthesis of theacrine. There results provided a reference for the synthesis of theacrine in tea plant.

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备注/Memo

备注/Memo:
收稿日期 Received: 2020-05-22 接受日期 Accepted: 2020-07-09
福建省“2011协同创新中心”中国乌龙茶产业协同创新中心专项（闽教科〔2015〕75号）、茶学福建省高校重点实验室开放课题（KLTS2018002）和福建省属公益类科研院所基本科研专项（2017R1012-2）资助 Supported by the Fujian Province “2011 Collaborative Innovation Center” Chinese Oolong Tea Industry Innovation Center special project (J2015-75), the Open Project of Key Laboratory of Tea Science in Universities of Fujian Province(KLTS2018002) and the Basic Sci-tech Project of Provincial Public Welfare Scientific Research Institution of Fujian (2017R1012-2)
**通信作者 Corresponding author (E-mail: ynxtea@126.com; zhaofeng0591@qq.com)
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更新日期/Last Update: 2020-08-04