|本期目录/Table of Contents|

[1]谢彤彤 孙晓娇 吴凯旋 迟明妹 张培玉 邱艳玲**.酱酒丢糟沼气发酵特性及微生物群落特征[J].应用与环境生物学报,2021,27(02):1-11.[doi:10.19675/j.cnki.1006-687x.2020.05019]
 XIE Tongtong,SUN Xiaojiao,WU Kaixuan,et al.Anaerobic digestion characteristics and microbial community of maotai-flavor stillage bio gas fermentation*[J].Chinese Journal of Applied & Environmental Biology,2021,27(02):1-11.[doi:10.19675/j.cnki.1006-687x.2020.05019]
点击复制

酱酒丢糟沼气发酵特性及微生物群落特征()
分享到:

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
27卷
期数:
2021年02期
页码:
1-11
栏目:
研究论文
出版日期:
2021-04-25

文章信息/Info

Title:
Anaerobic digestion characteristics and microbial community of maotai-flavor stillage bio gas fermentation*
作者:
谢彤彤 孙晓娇 吴凯旋 迟明妹 张培玉 邱艳玲**
青岛大学环境科学与工程学院 青岛 266071
Author(s):
XIE Tongtong SUN Xiaojiao WU Kaixuan CHI Mingmei ZHANG Peiyu & QIU Yanling**
School of Environmental Science and Engineering, Qingdao University, Qingdao 266071, China
关键词:
酱香白酒丢糟厌氧发酵WWE1中间代谢产物微生物群落
Keywords:
maotai-flavor stillage anaerobic digestion WWE1 intermediate metabolites microbial community
DOI:
10.19675/j.cnki.1006-687x.2020.05019
摘要:
酒糟是良好的沼气发酵原料,但其发酵中间产物极易累积导致体系酸化。采用批次实验研究中温条件下酱酒丢糟沼气发酵特性。以含固率TS分别为4.3%和7.8%的酱香白酒丢糟为发酵原料,在发酵30天和50天时,甲烷产量分别为287 mL/g TS 和360 mL/g TS ;TS去除率分别为20.8% 和27.4%。酱酒糟发酵过程中主要的中间代谢产物有乙酸、丙酸和丁酸等挥发性有机酸;此外,还检测出芳香族化合物有苯酚、对甲酚、苯甲酸和苯丙酸;其中丙酸在酒糟降解过程中明显累积(2894-4495 mg/L),推测丙酸累积为影响反应器稳定运行的重要因素。利用高通量测序技术对两组中温厌氧酒糟驯化培养系JO和JN进行解析。细菌结果表明,拟杆菌门(Bacteroidetes)、厚壁菌门(Firmicutes)、互养菌门(Synergistetes)和阴沟单胞菌门“Cloacimonetes”(WWE1)为主要的优势菌群。阴沟单胞菌门“Cloacimonetes”中未培养W5属和W27属在酒糟厌氧消化系统中占比为13.8%-30.67%。古菌解析检测到乙酸营养型产甲烷菌Methanosarcina mazei及氢营养型产甲烷菌Methanofollis ethanolicus近缘微生物为优势古菌。本研究表明丙酸累积是影响酱酒丢糟沼气发酵稳定性的重要因素,阴沟单胞菌门“Cloacimonetes”未培养微生物在酒糟厌氧发酵体系中发挥重要作用。
Abstract:
Vinasse is easy to acidify and produces a large number of volatile organic acids (VFAs), which is good fermentation material for biogas production. To study characteristics of anaerobic digestion with vinasse, batch experiments were conducted. Culture and molecular?techniqueswere used to explore the microbial diversity. Maotai-flavor stillage with solid concentration of 4.3% and 7.8% were used as sole carbon sources. Within 30 days and 50 days fermentation, the cumulative biogas yields were 287 mL/g TS and 360 mL/g TS, the total solids (TS) removal rate s w ere 20.8% and 27.4%, respectively. During anaerobic d igestion of stillage, acetate, propionate, butyrate were detected as main intermediate metabolites. Besides, aromatic compounds, such as p-cresol, phenylpropionate, phenol and benzoate were also detected as transient intermediates. Propionate accumulated significantly during the degradation of distiller’s grains (2894-4495 mg/L), which suggests that propionate accumulation has become an important factor restricting the stable operation of anaerobic reactors . The bacterial and archaeal community structures were investigated with a 16S rRNA gene-based high-throughput sequencing technology. The results showed that phyl a Bacteroidetes, Firmicutes, Synergistetes and Candidatus “ Cloacimonetes”(WWE1)were the most frequently detected. Cloacimonetes members of W5 and W27 genera accounted for 13.8% - 30.67%. Methanosarcina mazei, an acetate-trophic methanogen, and Methanofollis ethanolicus, a hydrogen-trophic methanogen, are dominant archaea and play important roles in methanogenesis. These results demonstrated propionate accumulation has become an important factor restricting the stable operation of anaerobic reactors, and Candidatus “ Cloacimonetes” uncultured microbes play an important role in the anaerobic digestion of Maotai-flavor stillage.

相似文献/References:

[1]靳孝庆,徐昊,沈磊,等.Clostridium papyrosolvens厌氧发酵产氢研究[J].应用与环境生物学报,2008,14(01):104.
 JIN Xiaoqing,et al..Hydrogen Production from Anaerobic Fermentation by Clostridium papyrosolvens[J].Chinese Journal of Applied & Environmental Biology,2008,14(02):104.
[2]石峰,刘和,聂艳秋,等.产氢产酸/同型产乙酸两相耦合工艺厌氧发酵市政污泥生产乙酸[J].应用与环境生物学报,2008,14(06):814.[doi:10.3724/SP.J.1145.2008.00814]
 SHI Feng,LIU He**,NIE Yanqiu,et al.Acetate Production from Sewage Sludge by a Novel Coupled Syntrophic Acetogenesis with Homoacetogenesis Process[J].Chinese Journal of Applied & Environmental Biology,2008,14(02):814.[doi:10.3724/SP.J.1145.2008.00814]
[3]李东,袁振宏,孙永明,等.城市生活垃圾有机组分厌氧发酵产氢和甲[英 文][J].应用与环境生物学报,2009,15(02):250.[doi:10.3724/SP.J.1145.2009.00250]
 LI Dong,YUAN Zhenhong**,et al.Sequential Anaerobic Fermentative Production of Hydrogen and Methane from Organic Fraction of Municipal Solid Waste[J].Chinese Journal of Applied & Environmental Biology,2009,15(02):250.[doi:10.3724/SP.J.1145.2009.00250]
[4]王佳婧,刘晓风,袁月祥,等.厌氧纤维素降解产氢复合菌系L-3的研究[J].应用与环境生物学报,2010,16(01):104.[doi:10.3724/SP.J.1145.2010.00104]
 WANG Jiajing,LIU Xiaofeng,YUAN Yuexiang,et al.Cellulose Degradation and Hydrogen Production by Anaerobic Microbial Consortium L-3[J].Chinese Journal of Applied & Environmental Biology,2010,16(02):104.[doi:10.3724/SP.J.1145.2010.00104]
[5]钱丹丹,马隆龙,袁月祥,等.一株嗜碱产甲烷八叠球菌的分离鉴定与系统发育分析[J].应用与环境生物学报,2012,18(02):262.[doi:10.3724/SP.J.1145.2012.00262]
 QIAN Dandan,MA Longlong,YUAN Yuexiang,et al.Isolation, Identification and Phylogenetic Analysis of an Alkalophilic Methanosarcina[J].Chinese Journal of Applied & Environmental Biology,2012,18(02):262.[doi:10.3724/SP.J.1145.2012.00262]
[6]赵亚,刘志军,刘凤霞,等.CO厌氧发酵制氢过程中的微生物特性及其影响因素[J].应用与环境生物学报,2012,18(04):656.[doi:10.3724/SP.J.1145.2012.00656]
 ZHAO Ya,LIU Zhijun,LIU Fengxia,et al.Microbial Characteristics and Influence Factors During Anaerobic Fermentation for Biohydrogen Production from CO[J].Chinese Journal of Applied & Environmental Biology,2012,18(02):656.[doi:10.3724/SP.J.1145.2012.00656]
[7]蒋心茹 刘杨 谢炎东 姬高升 许力山 宦臣臣 张忠武 刘明刚 闫志英**.回流液对剩余污泥厌氧消化的影响[J].应用与环境生物学报,2021,27(01):1.[doi:10.19675/j.cnki.1006-687x.2020.02035]
 JIANG Xinru,LIU Yang,XIE Yandong,et al.Effect of leachate recirculation on sewage sludge anaerobic digestion*[J].Chinese Journal of Applied & Environmental Biology,2021,27(02):1.[doi:10.19675/j.cnki.1006-687x.2020.02035]

备注/Memo

备注/Memo:

收稿日期 Received: 2020-05-09 接受日期 Accepted: 2020-06-11
国家自然科学基金项目(51878363)、山东省自然科学基金 (ZR2019MEE028)及宁夏回族自治区重点研发计划资助
**通讯作者 Corresponding author (E-mail: qiuyl@qibebt.ac.cn)
点击摘要页题目后的“PDF”可下载阅读全文;本文为已录用的作者修定稿,尚未经编辑全面修改。
引用本文请注明出处本刊;发表刊期和页码将以正式出版时的安排为准,但DOI确定不变。

 

更新日期/Last Update: 2020-07-03