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[1]温苗 施松梅 罗协 何新华 徐云飞 胥晓**.不同树龄桑园丛枝菌根侵染、球囊霉素土层分布及与土壤因子的关系[J].应用与环境生物学报,2021,27(02):1-16.[doi:10.19675/j.cnki.1006-687x.2020.05003]
 WEN Miao,SHI Songmei,LUO Xie,et al.Relationships between spatial distribution of arbuscular mycorrhizal colonization or glomalin-related soil protein and rhizhospheric soil chemical properties under different aged mulberry orchards[J].Chinese Journal of Applied & Environmental Biology,2021,27(02):1-16.[doi:10.19675/j.cnki.1006-687x.2020.05003]
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不同树龄桑园丛枝菌根侵染、球囊霉素土层分布及与土壤因子的关系()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
27卷
期数:
2021年02期
页码:
1-16
栏目:
研究论文
出版日期:
2021-04-25

文章信息/Info

Title:
Relationships between spatial distribution of arbuscular mycorrhizal colonization or glomalin-related soil protein and rhizhospheric soil chemical properties under different aged mulberry orchards
作者:
温苗1 施松梅1 罗协1 何新华1 徐云飞2 胥晓2**
1 西南大学资源环境学院重庆400715
2 西华师范大学生命科学学院南充 637002
Author(s):
WEN Miao1 SHI Songmei1 LUO Xie1 HE Xinhua1** XU Yunfei2 & XU Xiao2
1 Centre of Excellence for Soil Biology, College of Resources and Environment, Southwest University, Chongqing 400716, China
2 College of Life science, China West Normal University, Nanchong 637002, China
关键词:
丛枝菌根真菌土壤化性状球囊霉素
Keywords:
Morus alba arbuscular mycorrhizal fungi soil chemical properties glomalin urease sucrase
DOI:
10.19675/j.cnki.1006-687x.2020.05003
摘要:
桑树(Morus alba L.)对丛枝菌根真菌Arbuscular mycorrhiza fungi,AMF)具有较高的依赖性但少见桑树丛枝菌根AMF)侵染、球囊霉素相关土壤蛋白的研究。四川省宜宾市兴文县不同树龄桑树(1、2、3和4年生)侵染、土层球囊霉素相关土壤蛋白变化与土壤主要无机养分的相关关系。0-30 cm土层桑树根系菌丝侵染率、0-0 cm 和10-30 cm土壤易提取球囊霉素相关土壤蛋白(EE-GRSP)和总球囊霉素(T-GRSP)随树龄的增加都呈先上升后下降的趋势,峰值都出现在2年生桑园。土壤pH、有机质、速效钾、脲酶、蔗糖酶活性在2年龄桑园达到最高,有效磷在3年龄桑园最高,而碱解氮高低与桑树龄无关。冗余分析表明显著影响根系AMF侵染的主要土壤因子排序为T-GRSP >脲酶> pH > EE-GRSP(P < 0.05)。多元线性回归分析表明EE-GRSP和T-GRSP含量均与土壤pH、脲酶、蔗糖酶、有机质、速效钾呈显著正相关(P < 0.05),而与碱解氮、泡囊和丛枝侵染率无显著相关;此外,EE-GRSP还与土壤有效磷和菌丝侵染率呈显著正相关(P < 0.05)。研究结果表明1、2、3和4年龄桑树都能与AMF形成丛枝菌根共生关系土壤pH、脲酶直接影响AMF侵染,而蔗糖酶通过影响EE-GRSP和T-GRSP含量来间接影响AMF侵染因此,提高植物根系丛枝菌根形成和土层球囊霉素相关土壤蛋白含量对于土壤促进宿主植物生长发育具有重要作用。
Abstract:
Mulberry (Morus alba L.) is a highly arbuscular mycorrhizal fungus (AMF) dependent species. However, little information is available for relationships between mycorrhizal colonization, GRSP (glomalin-related soil protein) and soil chemical properties in mulberry orchards. In this study, root colonization rate, GRSP, soil nutrients and activity of urease and sucrase were examined from different ages (1-, 2-, 3- and 4-years) of mulberry orchards in Xingwen County, Yibin City, Sichuan Province, and then related these measurements to possible relationships. Results showed that root AMF colonization rates and soil GRSP concentrations at soil depth of 0-10 cm and 10-30 cm were firstly increased and then decreased with the increase of mulberry ages from 1-, 2-, 3- to 4-years, while their peak values appeared in the 2-year-old mulberry orchard. Soil pH, soil organic matter (SOM), available potassium (AK), activity of urease and sucrase were maximal in the two-year-old mulberry orchard. In contrast, the value of soil available phosphorus (AP) peaked in the three-year-old mulberry orchard, and soil available nitrogen (AN) was similar between different mulberry orchards. Redundancy analyses showed that the major soil factors with significant effects on root AMF colonization were T-GRSP > urease > pH > EE-GRSP (P < 0.05). Correlation analyses showed both EE-GRSP and T-GRSP significantly positively related to soil pH, SOM, urease, sucrase and AK, but not with AN, root vesicular and mycorrhizal colonization rate. In contrast, EE-GRSP related to AP and hyphal colonization rate. In summary, our results demonstrated that roots from all tested mulberry orchards had formed AMF associations. Root AMF colonization was directly affected by soil pH and urease activity, but indirectly by sucrase activity, which affected the EE-GRSP and T-GRSP production. The increase of soil inorganic NPK positively related to the increase of GRSP. As a result, an improvement of both AM formation and GRSP could play an important role in promoting soil fertility and host growth as well as practice in restoration ecology.

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备注/Memo

备注/Memo:
收稿日期 Received: 2020-05-03 接受日期 Accepted: 2020-06-28
四川省科技厅省院省校科技合作重点项目(2018JZ0027)、国家自然科学青年基金(4111800096)、国家重点研发计划子课题“果树养分推荐方法与限量标准”(2016YFD0200104)和西南大学生物学研究中心“国家生物学双一流学科”建设和植物资源保护与种质创新重庆市重点实验室研究经费资助
**通讯作者 Corresponding authors (E-mail: xinhua.he@uwa.edu.au; xuxiao_cwnu@163.com)
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更新日期/Last Update: 2020-07-03