|本期目录/Table of Contents|

 LI Xiaofei,ZHANG Shuting,SHEN Xu,et al.Genome-wide identification and expression analysis of HAT gene family in longan[J].Chinese Journal of Applied & Environmental Biology,2021,27(02):1-12.[doi:10.19675/j.cnki.1006-687x.2020.04051]





Genome-wide identification and expression analysis of HAT gene family in longan
福建农林大学园艺植物生物工程研究所 福州 350002
LI Xiaofei ZHANG Shuting SHEN Xu ZHANG Zihao CHEN Yukun LIN Yuling &LAI Zhongxiong**
Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, C hina
Dimocarpus longan Lour. Acetylase gene genome-wide identification epigenetic regulation Somatic embryogenesis expression pattern
组蛋白乙酰化酶(Histone acetylase,HAT)是组蛋白赖氨酸残基共价修饰的组成部分,在表观遗传调控过程发挥重要作用。为了解龙眼HAT基因(DlHAT)家族的生物学功能及表达模式,基于龙眼基因组及转录组数据对DlHAT基因家族成员进行全基因组鉴定及生物信息学分析,利用qRT-PCR检测DlHAT在龙眼体胚发生早期和不同激素处理下的表达情况。结果显示:DlHAT基因家族包含6个成员:DlHAC1、DlHAF1、DlHAM1、DlHAG1、DlHAG2及DlHAG3,分布于6条染色体上,均为亲水性蛋白质。亚细胞定位预测显示DlHAT分布于细胞核和细胞质中;进化树聚类显示DlHAT家族可分为HAFs、HAGs、HACs和HAMs四类,其中DlHAGs家族包含GCN5、ELP3和HAT1三个亚家族;基因结构分析发现DlHAT外显子个数在8-30个不等;DlHAT大部分编码蛋白含有motif1,除HAGs亚家族外HAFs、HACs和HAMs亚家族编码蛋白均含有motif6,其蛋白结构含α–螺旋数及无规则卷曲数较多;DlHAT启动子序列中包含大量光、激素、逆境胁迫及生长发育相关的顺式作用元件。DlHAT在龙眼体胚发生早期及不同激素处理下的qRT-PCR分析显示:DlHAF1、DlHAG1、和DlHAM1在球形胚阶段表达量较高;除DlHAF外其他成员均响应水杨酸(SA)、茉莉酸甲酯(MeJA)、脱落酸(ABA)和赤霉素(GA3)的调控,在SA、GA3处理下均上调表达,且在SA处理8 h时和GA3处理4 h时表达量均显著上调。本研究表明DlHAT在进化过程中存在保守性与物种特异性,且可能通过响应激素调控而参与龙眼体胚发育。
Histone acetylase (HAT) is a component of Histone lysine residue covalent modification and plays an important role in epigenetic regulation. To investigate the biological function and expression patterns of the HAT gene family in Dimocarpus longan Lour, this study conducted genome-wide identification and bioinformatics analysis of longan HAT gene family( DlHAT) b ased on longan genome and transcriptome dataset, and detected t he expression patterns of DlHAT in the early somatic embryogenesis and different hormone treatments of longan by qRT-PCR.The results showed that the DlHAT gene family contain 6 members, named DlHAC1, DlHAF1, DlHAM1, DlHAG1, DlHAG2 and DlHAG3. They were?distributed on 6 chromosomes of longan , and all of them were hydrophilic proteins . Subcellular localization prediction showed that DlHAT was distributed in the nucleus and cytoplasm. Phylogenetic tree analysis showed that the DlHAT family can be divided into four categories: HAFs, HAGs, HACs and HAMs, and the DlHAGs family could further be divided into three subfamilies: GCN5, ELP3 and HAT1. Gene structure analysis found that the number of exons ranged from 8 to 30 in DlHAT family, and most of their encoded proteins contained motif1 , except for HAGs subfamily, the HAFs, HACs, and HAMs subfamily encoded proteins contained motif6, the protein structure contained α-helix and random coils. The DlHAT promoter sequence contained a lot of light, hormone, stress, growth and development related cis-acting elements. qRT-PCR analyses of the early somatic embryos and different hormone treatments in longan revealed that DlHAF1, DlHAG1, and DlHAM1 were expressed at higher levels in the globular embryo (GE) stage; Except for DlHAF, other DlHAT genes responded to the salicylic acid (SA), methyl jasmonate (MeJA), abscisic acid (ABA) and gibberellin (GA3) hormones.Moreover, the expression were up-regulated under the treatment of SA and GA 3, and the expression levels were significantly upregulated at 8 h SA treatment and 4 h GA3 treatment. This study indicated that DlHAT is conservative and species-specific characteristics during the evolution process , and may be involved in longan somatic embryonic development by responding to hormone regulation.


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收稿日期 Received: 2020-04-27 接受日期 Accepted: 2020-06-01 国家自然科学基金项目(31572088,31672127)、福建省高原学科建设经费(102/71201801101)和福建农林大学科技创新专项基金(CXZX2017189、CXZX2017314、CXZX2018076、KF2015108)资助
**通讯作者 Corresponding author (E-mail : Laizx01@163.com)
更新日期/Last Update: 2020-07-03