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[1]李晶晶 谭力 方扬 靳艳玲 易卓林 杜安平 何开泽** 赵海**.高硝氮酒厂废水生物反硝化体系的构建及细菌群落特征[J].应用与环境生物学报,2021,27(02):1-13.[doi:10.19675/j.cnki.1006-687x.2020.04024]
　LI Jingjing,TAN Li,FANG Yang,et al.Construction and bacterial community of biological denitrification system for high-nitrate wastewater from winery[J].Chinese Journal of Applied & Environmental Biology,2021,27(02):1-13.[doi:10.19675/j.cnki.1006-687x.2020.04024]
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高硝氮酒厂废水生物反硝化体系的构建及细菌群落特征()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
27卷
期数:
2021年02期
页码:
1-13
栏目:
研究论文
出版日期:
2021-04-25

文章信息/Info

Title:
Construction and bacterial community of biological denitrification system for high-nitrate wastewater from winery
作者:
李晶晶1; 2; 3 谭力1; 3; 4 方扬1; 3 靳艳玲1; 3 易卓林1; 3 杜安平1; 3 何开泽1; 3** 赵海1; 3**
1中国科学院成都生物研究所 成都 610041
2中国科学院大学 北京 100049
3中国科学院环境与应用微生物重点实验室 成都 610041
4中国轻工业浓香型白酒固态发酵重点实验室 宜宾 644000
Author(s):
LI Jingjing1; 2; 3;  TAN Li1; 3; 4;  FANG Yang1; 3;  JIN Yanling1; 3;  YI Zhuolin1; 3;  DU Anping1; 3;  HE Kaize1; 3**& ZHAO Hai1; 3**
1Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China
2University of Chinese Academy of Sciences, Beijing 100049, China
3Key Laboratory of Environmental and Applied Microbiology of Chinese Academy of Sciences, Chengdu 610041, China
4Key Laboratory of Chinese light industry Strong flavor liquor solid state fermentation, Yibin 6 44000, China
关键词:
高硝氮废水; 碳源; MicroC; 厌氧反硝化; 细菌群落结构
Keywords:
high-nitrate wastewater;  anaerobic denitrification;  carbon source;  MicroC;  bacterial community structure
DOI:
10.19675/j.cnki.1006-687x.2020.04024
摘要:
白酒生产过程中伴随高氮废水的产生，其中包含氨氮（NH4+-N）、硝氮（NO3--N）和亚硝氮（NO2--N），企业基于现有的曝气等工艺可以去除NH4+-N，但却难以有效去除NO3--N和NO2--N，导致总氮（TN）含量无法达到新标准（TN < 20 mg L-1），因此高效去除废水中的NO3--N和NO2--N成为当下的研究热点。采用上流式厌氧污泥床（Up-flow Anaerobic Sludge Blanket ,U ASB）生物反应器驯养活性污泥，形成稳定的微生物群系；筛选得到最佳碳源，构建了生物厌氧反硝化脱氮体系，并通过三代全长16S rRNA测序分析了体系的细菌群落结构。结果显示，在甲醇、乙酸钠、丁二酸钠、葡萄糖、酒厂原水、柠檬酸钠和MicroC多种碳源中，MicroC效果最佳，在处理高硝氮废水（NO3--N = 531 mg L-1）时，添加量为C/N = 1.0，出水的NO3--N含量小于1 mg L -1，NO3--N去除率达98%，COD去除率超过90%。该体系中，反硝化前期斯氏假单胞菌（Pseudomonas stutzeri）和硫杆菌（Thioclava sp.）是优势种，还原大量的NO3--N，而细菌多样性较低；反硝化后期微嗜酸寡养单胞菌（Stenotrophomonas acidaminiphila）变成优势种，还原残留的NO3--N。本研究表明以MicroC为碳源的厌氧反硝化体系可实现酒厂高硝氮废水低成本且高效率的脱氮处理，物种Pseudomonas stutzeri发挥主要的反硝化作用，对反硝化工程有重要的指导意义。
Abstract:
The winery wastewater is required that its total nitrogen content （TN）must be less than 20 mg L -1 before discharge. The TN of this kind of wastewater composed of NH4+-N, NO3--N and NO2--N. Based on the existing techniques such as aeration treatment, NH 4+-N can be removed easily, but NO3--N and NO2--N is difficult to be treated by ordinary methods, leading to the content of total nitrogen cannot meet the standard. Therefore, the efficient removal of nitrate in wastewater become a technical focus. In this study, anaerobic biological denitrification technology was used to remove nitrate in this wastewater. Firstly, the denitrifying microbial community was established by activated sludge domestication and enrichment in a self-designed U ASB reactor. Then the different carbon sources promoting the efficiency of denitrification were screened and quantified. Finally, the bacterial diversity of the system was analyzed through third-generation sequencing technology. The results showed that among the different carbon sources of methanol, sodium acetate, sodium succinate, glucose, winery raw water, sodium citrate and MicroC, MicroC was the best for denitrification. The actual target wastewater from winery (TN = 531 mg L-1) was treated by the method of this study, the NO3--N content of the effluent was less than 1 mg L -1, and its removal rate reached 98%, and the COD removal rate exceeded 90% when MicroC was used as carbon source and the C/N = 1.0. This result of sequencing showed that the microbial community structure changed as the denitrification progresses . In the early stage of denitrification, Pseudomonas stutzeri and Thioclava sp. are the dominant species to reduce a large amount of nitrate, and the diversity of bacteria was relatively low . In the later period, Stenotrophomonas acidaminiphila became the dominant species to reduce residual nitrate . These results demonstrated the anaerobic denitrification system that took MicroC as the carbon source can realize the low-cost and high-efficiency biological treatment of high-nitrate wastewater of winery, and Pseudomonas stutzeri played a major role in this system, these have important significance for denitrification projects.

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[1]冯瑞章,姚拓,周万海,等.不同生存环境和磷酸盐对4株溶磷菌溶磷能力的影响[J].应用与环境生物学报,2009,15(06):856.[doi:10.3724/SP.J.1145.2009.00856]
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备注/Memo

备注/Memo:
收稿日期 Received: 2020-04-12 接受日期 Accepted: 2020-05-06
四川省科技计划项目（2017HH0077）资助 Supported by Project of Science and Technology of Sichuan, China (2017HH0077)
**通讯作者 Corresponding author (E-mail: hekz@cib.ac.cn, zhaohai@cib.ac.cn)
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更新日期/Last Update: 2020-05-22