|本期目录/Table of Contents|

[1]林沚葳,叶秀云,林娟,等.固定化漆酶反应器的构建及应用[J].应用与环境生物学报,2021,27(02):1-10.[doi:10.19675/j.cnki.1006-687x.2020.04015]
 LIN Zhiwei,YE Xiuyun,LIN Juan,et al.Construction and application of immobilized laccase reactor*[J].Chinese Journal of Applied & Environmental Biology,2021,27(02):1-10.[doi:10.19675/j.cnki.1006-687x.2020.04015]
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固定化漆酶反应器的构建及应用()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
27卷
期数:
2021年02期
页码:
1-10
栏目:
研究快报
出版日期:
2021-04-25

文章信息/Info

Title:
Construction and application of immobilized laccase reactor*
作者:
林沚葳叶秀云林娟杨捷**
福州大学福建省海洋酶工程重点实验室 福州 350116
Author(s):
LIN Zhiwei YE Xiuyun LIN Juan YANG Jie**
Fujian Key Laboratory of Marine Enzyme Engineering, Fuzhou University, Fuzhou 350116, China
关键词:
漆酶壳聚糖固定化酶反应器孔雀石绿
Keywords:
Laccase Enzyme immobilization Bioreactor Malachite green
DOI:
10.19675/j.cnki.1006-687x.2020.04015
摘要:
漆酶具备较强的工业应用潜力,但经济性和稳定性的不足限制了其推广应用,酶的固定化被认为是提高漆酶稳定性的一种有效方法。以来自Cerrena sp. HYB07 菌株的漆酶作为研究对象,以壳聚糖为载体制备固定化漆酶,构建实验室规模的酶反应器并应用于染料脱色,探讨固定化漆酶的应用效果。结果显示,以愈创木酚为底物,优化固定化漆酶的制备条件,所得固定化漆酶的最高酶活力为43.5 U/g。固定化后漆酶的热稳定性增强,65 ℃ 条件下固定化酶半衰期是游离酶的5.3倍。将固定化漆酶填充于酶反应器中处理Remazol Brilliant Blue Reactive (RBBR)和孔雀石绿,酶反应器由进样池、反应池和出样池构成,设置为批次和循环两种模式。批次模式下,重复使用10次,固定化漆酶仍可保留60%以上的酶活力及脱色率。与批次模式相比,循环模式下固定化漆酶对染料的脱色效率更高,固定化酶表现出了比游离酶更好的操作稳定性,可实现对孔雀石绿的完全脱色。经漆酶处理后,孔雀石绿的微生物毒性、植物毒性和细胞毒性都显著降低。本研究表明固定化可以提高漆酶的稳定性和使用效率,研究结果可为漆酶在染料脱色脱毒方面的应用提供理论依据。
Abstract:
Laccase is valuable for an extensive variety of industrial applications. Nonetheless, large-scale applications of laccases are limited by the economy and efficiency of the enzymes. Enzyme stability can be improved through immobilization. In the present study, the laccase from Cerrena sp. HYB07 was covalently immobilized on chitosan and continuous decolorization of dyes was achieved with an lab-scale bioreactor.With guaiacol as the substrate, the activity of immobilized enzyme was 43.5 U/g after optimization of immobilization conditions . Immobilized laccase displayed higher thermostability, with a half-life time at 65 ℃ 5.3 times of that of the free enzyme . The reactor was composed of injection cell, a reaction vessel and a collector, set to batch and cycle modes. The immobilized enzyme was used in a enzyme reactor to decolorize Remazol Brilliant Blue Reactive (RBBR) and Malachite green (MG). A fter ten decolorization cycles, the activity of immobilized laccase and the decolorization rate s of dyes remained at 60% . Compared with the batch mode, the decolorization efficiency of the immobilized enzyme was higher in the circulating mode . Decolorization of MG by immobilized enzyme was more thorough than that of free enzyme . After laccase treatment, the microbial toxicity, phytotoxicity and cytotoxicity of MG were significantly reduced. The stability and decolorization efficiency of laccase was improved after immobilization . Furthermore, the immobilized laccase has better operational stability in a bioreactor. Results of this study can prove the application value of laccase in dye decolorization and detoxification.

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备注/Memo

备注/Memo:
收稿日期 Received: 2020-04-08 接受日期 Accepted: 2020-06-05
国家自然科学基金(31671795)资助
**通讯作者 Corresponding author (E-mail: T09136@fzu.edu.cn)
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更新日期/Last Update: 2020-07-03