|本期目录/Table of Contents|

[1]方源 谢柏盛 黄婷 郑鑫 许子牧** 汪家权**.34株假单胞菌的泛基因组分析[J].应用与环境生物学报,2021,27(01):1-13.[doi:10.19675/j.cnki.1006-687x.2020.03048]
 Fang Yuan,Xie Baisheng,Zheng Xin,et al.Pan-genomic characteristic of 34 Pseudomonas strains[J].Chinese Journal of Applied & Environmental Biology,2021,27(01):1-13.[doi:10.19675/j.cnki.1006-687x.2020.03048]
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34株假单胞菌的泛基因组分析()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
27卷
期数:
2021年01期
页码:
1-13
栏目:
研究论文
出版日期:
2021-02-25

文章信息/Info

Title:
Pan-genomic characteristic of 34 Pseudomonas strains
作者:
方源1 谢柏盛2 黄婷1 郑鑫1 许子牧1** 汪家权1**
1合肥工业大学资源与环境工程学院 合肥 230009
2北京大学工学院 北京 100871
Author(s):
Fang Yuan1 Xie Baisheng2 Zheng Xin 1 Xu Zimu 1** & Wang Jiaquan1**
1 School of Resources and Environmental Engineering, Hefei University of Technology, Hefei 230009, China
2 College of Engineering , Peking University, Beijing 100871, China
关键词:
假单胞菌属泛基因组核心基因组环境适应
Keywords:
Pseudomonas pa n-genome core genome environmental adaptation
DOI:
10.19675/j.cnki.1006-687x.2020.03048
摘要:
假单胞菌属在各种环境广泛分布且具有重要的生态应用价值,然而当前研究对其环境适应性遗传基础的解析尚不充分。选择33株有效发表且具有全基因组序列以及一株新分离的假单胞菌的全基因组进行泛基因组学分析。结果表明,文中选择的假单胞菌属菌株泛基因组含有173 271条蛋白序列,共有30 847个同源基因家族,包括1 505个核心基因,基因组呈开放型。通过分析不同基因组的基因组演化特征,发现通过基因水平转移获得的基因占整个基因组的比例为 32%-48%,其平均基因岛数量为53个。发现假单胞菌属泛基因组的开发性与其频繁的基因水平转移事件和快速的基因进化有关。进一步分析表明,不同基因组中的功能基因家族按照菌株的环境分布进行聚类,表明对特殊环境的适应决定了假单胞菌的遗传特征。本研究初步揭示了假单胞菌属细菌遗传特征的共性与特性,可为解析假单胞菌属环境适应性的遗传基础与功能多样性的演化发生机制提供研究线索。(图8表1参37)
Abstract:
Members of Pseudomonas are widely distributed in various environments, and has important ecological application potentials. However, the genetic basis of its environmental adaptation is still unclear. In this work, we performed pan-genomic analysis of Pseudomonas based on 33 complete genomes from GenBank and an additional genome of a newly isolated Pseudomonas strain. The results show that the 34 Pseudomonas strains have a pan-genome consisting of 173,271 predicted genes grouped into 30,847 homologous gene families, including a core genome consisting of 1,505 genes. The pan-genome of the studied Pseudomonas strains is open, which may be derived from the combined effects of horizontal gene transfer (HGT) and rapid evolution. About 32% - 48% of genes in Pseudomonas genomes are obtained through HGT, and the average number of genomic islands is 53 per genome. Further analyses show that functional gene families in different genomes are clustered according to their isolation environments, which indicates that adaptation to a special environment determines the genetic characteristics of Pseudomonas. This study initially revealed the genetic characteristics of Pseudomonas strains, and provided clues for the genetic basis of environmental adaptation and the evolutionary mechanisms of functional diversity.

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备注/Memo

备注/Memo:
收稿日期 Received: 2020-03-25 接受日期 Accepted: 2020-05-06
国家自然科学青年科学基金(51807046)项目 Supported by the National Natural Science Foundation of China (51807046)
**通讯作者 Corresponding author (E-mail: jiaquan.wang@163.com; xzm666@hfut.edu.cn)
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更新日期/Last Update: 2020-05-22