|本期目录/Table of Contents|

[1]刘小娜 李彪 唐晨 孙佳栋 吴夏芫 周俊 雍晓雨**.Quorum Sensing信号分子对Pseudomonas aeruginosa PAO1 生物膜结构及产电性能的影响[J].应用与环境生物学报,2021,27(02):1-12.[doi:10.19675/j.cnki.1006-687x.2020.03047]
 LIU Xiaona,LI Biao,TANG Chen,et al.Effects of quorum sensing (QS) signal molecules on biofilm formation and electricity generation performance of Pseudomonas aeruginosa PAO1[J].Chinese Journal of Applied & Environmental Biology,2021,27(02):1-12.[doi:10.19675/j.cnki.1006-687x.2020.03047]
点击复制

Quorum Sensing信号分子对Pseudomonas aeruginosa PAO1 生物膜结构及产电性能的影响()
分享到:

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
27卷
期数:
2021年02期
页码:
1-12
栏目:
研究论文
出版日期:
2021-04-25

文章信息/Info

Title:
Effects of quorum sensing (QS) signal molecules on biofilm formation and electricity generation performance of Pseudomonas aeruginosa PAO1
作者:
刘小娜 李彪 唐晨 孙佳栋 吴夏芫 周俊 雍晓雨**
南京工业大学生物与制药工程学院 南京 211800
Author(s):
LIU Xiaona LI Biao TANG Chen SUN Jiadong WU Xiayuan ZHOU Jun YONG Xiaoyu**
College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University , Nanjing 211800, China
关键词:
群体感应 信号分子 铜绿假单胞菌 生物膜 产电性能
Keywords:
quorum sensing (QS) signal molecule Pseudomonas aeruginosa biofilm bioelectrochemical performance
DOI:
10.19675/j.cnki.1006-687x.2020.03047
摘要:
电活性生物膜(EAB)是微生物燃料电池(microbial fuel cell,MFC)的重要组成部分,为揭示群体感应(Quorum Sensing, QS)信号分子在MFC中对EAB的响应机制。在MFC阳极室接种铜绿假单胞菌,添加两种QS信号分子(N-丁酰基丝氨酸内酯和喹诺酮),观察其对阳极生物膜形态、结构及MFC产电性能的影响。结果显示添加终浓度为10 μmol/L N- 丁酰基丝氨酸内酯(C4-HSL)、喹诺酮(PQS)的MFC的驯化启动期分泌比对照组缩短了290.33 h、169.9 h ,最高输出电压分别提高了18.18%、22.73%, MFC运行后期传质电阻分别减小了9.77 Ω 、15.15 Ω,绿脓素含量分别提高了20.27%、24.32%;电子显微镜(SEM)照片显示两种添加信号分子生物膜的生物量均多于对照组;激光扫描共聚焦显微镜(CLSM)照片显示,添加QS信号分子可以明显增大阳极生物膜的厚度并改善活死细胞比例。本研究结果表明,添加QS信号分子显著促进了产电菌Pseudomonas aeruginosa PAO1 在MFC阳极表面的成膜速率,强化了其生物活性,提高EAB与电极间的电子传递效率及MFC的产电性能。
Abstract:
As an important component of microbial fuel cell (MFC), EAB is intended to reveal the response mechanism of Quorum Sensing (QS) signal molecules to EAB in MFC. Pseudomonas aeruginosa was inoculated in the MFC anode chamber, and two QS signaling molecules (N-butylserine lactone and quinolone) were added to observe their effects on the morphology, structure and electrical performance of the anode biofilm. Results showed that adding 10 ?mol/L N-butyl homoserine lactones (C4- HSL), quinolone (PQS) both decreased the start-up stages of the MFC than the control group by 290.33 h and 169.9 h, respectively; the highest output voltage is increased by 18.18% and 22.73% respectively; the mass transfer resistance reduced 9.77 Ω and 15.15 Ω respectively, pyocyanin content increased by 20.27% and 24.32% respectively . Electron microscopy photos showed that the EAB on the anode of the QS signal molecules added MFCs, which was dominated by bacillus, was richer than that of the control group. Laser scanning confocal microscopy (CLSM) photos showed that the addition of QS signal molecules significantly improved the proportion of living to dead cells in the EAB. The results here showed that the addition of QS signal molecules significantly promoted the biofilm-forming rate of electrochemical active bacteria on the anode, which strengthened its bioactivity, improved the electron transfer efficiency between EAB and electrodes, and thus enhanced the power generation performance of the MFC system.

相似文献/References:

[1]李珣,周存宇,田春元,等.枯草芽孢杆菌中多聚-γ-谷氨酸的生物合成及其调控机制[J].应用与环境生物学报,2013,19(04):575.[doi:10.3724/SP.J.1145.2013.00575]
 LI Xun,ZHOU Cunyu,TIAN Chunyuan,et al.Biosynthesis of Poly-γ-glutamic Acids and Relevant Regulatory Networks in Bacillus subtilis[J].Chinese Journal of Applied & Environmental Biology,2013,19(02):575.[doi:10.3724/SP.J.1145.2013.00575]
[2]陈国科,黄钧,毕京芳,等.好氧颗粒污泥耐受高碳氮负荷过程中的群体感应[J].应用与环境生物学报,2014,20(01):73.[doi:10.3724/SP.J.1145.2014.00073]
 CHEN Guoke,HUANG Jun,BI Jingfang,et al.Quorum sensing of aerobic granular sludge tolerating high carbon and nitrogen loads[J].Chinese Journal of Applied & Environmental Biology,2014,20(02):73.[doi:10.3724/SP.J.1145.2014.00073]
[3]黄昕琦,蔡中华,林光辉,等.群体感应信号对“藻-菌”关系的调节作用[J].应用与环境生物学报,2016,22(22卷04):708.[doi:10.3724/SP.J.1145.2016.02019]
 HUANG Xinqi,CAI Zhonghua,LIN Guanghui,et al.Quorum sensing modulating algae-bacteria interactions*[J].Chinese Journal of Applied & Environmental Biology,2016,22(02):708.[doi:10.3724/SP.J.1145.2016.02019]

备注/Memo

备注/Memo:
收稿日期 Received: 2020-03-23     接收日期 Accepted: 2020-05-21
江苏省先进生物制造协同创新中心自主研究课题(XTE1843)和宁夏回族自治区重点研发计划项目(2019BFH02008)资助
**通讯作者 Corresponding author (E-mail: yongxiaoyu@njtech.edu.cn)
点击摘要页题目后的“PDF”可下载阅读全文;本文为已录用的作者修定稿,尚未经编辑全面修改。
引用本文请注明出处本刊;发表刊期和页码将以正式出版时的安排为准,但DOI确定不变。
更新日期/Last Update: 2020-07-03