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[1]赵鹏程 徐小萍 张春渝 陈晓慧 申序 林玉玲 赖钟雄**.龙眼Aquaporin家族的全基因组鉴定及其在体细胞胚发生早期的表达[J].应用与环境生物学报,2021,27(02):1-21.[doi:10.19675/j.cnki.1006-687x.2020.03040]
 Zhao Pengcheng,Xu Xiaoping,Zhang Chunyu,et al.Genome-wide identification of Aquaporin family and expression during early somatic embryogenesis in Dimocarpus longan Lour*[J].Chinese Journal of Applied & Environmental Biology,2021,27(02):1-21.[doi:10.19675/j.cnki.1006-687x.2020.03040]
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龙眼Aquaporin家族的全基因组鉴定及其在体细胞胚发生早期的表达()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
27卷
期数:
2021年02期
页码:
1-21
栏目:
研究论文
出版日期:
2021-04-25

文章信息/Info

Title:
Genome-wide identification of Aquaporin family and expression during early somatic embryogenesis in Dimocarpus longan Lour*
作者:
赵鹏程 徐小萍 张春渝 陈晓慧 申序 林玉玲 赖钟雄**
福建农林大学园艺植物生物工程研究所 福州 350002
Author(s):
Zhao Pengcheng Xu Xiaoping Zhang Chunyu Chen Xiaohui Shen Xu Lin Yuling Lai Zhongxiong* *
Institute of horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
关键词:
龙眼Aquaporin基因家族全基因组鉴定功能分析
Keywords:
longan AQP gene family genome-wide identification functional analysis
DOI:
10.19675/j.cnki.1006-687x.2020.03040
摘要:
水孔蛋白(AQP)是生物调控水跨膜运输的重要通道。为了解龙眼AQP基因家族的生物学功能,利用本实验室的龙眼基因组数据库,结合生物信息学的分析方法进行龙眼全基因组范围内AQP家族成员的鉴定,并对其蛋白质理化性质、系统进化关系、共线性、选择压力、基因结构、保守基序、蛋白质保守结构域、顺式作用元件、蛋白质互作和体细胞胚早期不同阶段的基因表达情况进行分析。结果显示,龙眼AQP基因家族共有35个成员,蛋白分子量在17.77~44.30kD之间,分布在14条染色体上;经系统发育树分析,可将其分为5个不同亚家族:PIPs、TIPs、NIPs、SIPs和XIPs;不同亚家族间等电点、磷酸化位点、内含子数量和motif分布差异较大;AQP家族成员间共有6对共线性基因,与拟南芥基因组间有28对共线性基因,且在进化过程中都受纯化选择作用;龙眼AQP家族蛋白质都含有MIP保守结构域;其家族成员可与SYP、PP2A和BOR发生互作,PIP亚家族内的蛋白互作关系最强;基因组的FPKM值分析发现,大部分AQP成员可在龙眼胚胎早期不同阶段进行表达,其中DlTIP4的表达量在所有阶段远高于其他成员,可能在整个过程中发挥着重要作用。上述结果表明,龙眼35个AQP家族成员基因可能在体细胞胚胎发育阶段发挥重要作用,并且可能响应干旱、低温等非生物胁迫和脱落酸、生长素等植物激素的调控,在功能上具有明显的多样性。
Abstract:
Aquaporin (AQP) is an important protein channel for biological regulation of water transmembrane transport. In order to investigate biological function of longan AQP gene family, the longan genome database of our laboratory and bioinformatics analysis method s w ere used to identify the whole AQP family, including family numbers identification, protein physical and chemical properties, phylogenetic tree, collinearity, selection pressure, gene structure, conserved motif, conserved domain, promoter, protein interaction and gene expression in different stages of early somatic embryo. The results show that there are 35 members of longan AQP gene family, and the molecular weight of the protein ranged from 17.77kD to 44.30k D distributed on 14 chromosomes. According to phylogenetic tree analysis, the families can be divided into five different subfamilies: PIPs, TIPs, NIPs, SIPs and XIPs. The distribution of isoelectric point, phosphorylation site, intron number and motif among the different subfamilies were quite different. There are 6 pairs of collinearity genes between longan AQP gene families and 28 pairs of collinearity genes between longan AQP gene families and Arabidopsis genome, and they are all under purified selection during the evolutionary process. The conserved domain of the longan AQP family is MIP , and the family members can be associated with SYP, PP2A and BRO, especially in PIP subfamily. F PKM value analysis of genome showed that most AQP members can be expressed in different stages during the early embryo of longan, and the expression of DlTIP4 is much higher than other members in all stages during the early embryo , possibly play ing an important role in the whole process. All in all, this study shows that t he 35 AQP family members of longan may play an important role in the stage of somatic embryogenesis, and possibly responding to abiotic stress such as drought and low temperature, being regulated by abscisic acid, auxin and other plant hormones as well , showing obvious functional diversity.

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备注/Memo

备注/Memo:
收稿日期 Received: 2020-03-20 接受日期 Accepted: 2020-05-06
国家自然科学基金项目(31672127,31572088)、福建省高原学科建设经费(102/71201801101)和福建农林大学科技创新专项基金(CXZX2017189,CXZX2016118,CXZX2017314,CXZX2018076,KF2015108)资助 Supported by National Natural Science Foundation of China (3167212731572088), Fujian Province plateau discipline construction fund (102 / 71201801101), science and technology innovation special fund of Fujian agricultural and Forestry University (CXZX2017189; CXZX2016118, CXZX2017314,CXZX2018076, KF2015108)
**通讯作者 Corresponding author (E-mail: Laizx01@163.com)
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更新日期/Last Update: 2020-05-22