|本期目录/Table of Contents|

[1]安丽荣 卞文新 刘宝华 申天琳**.[综 述] 环境胁迫对氨氧化菌群的影响研究进展[J].应用与环境生物学报,2021,27(03):1-10.[doi:10.19675/j.cnki.1006-687x.2020.03005]
 AN Lirong,BIAN Wenxin,LIU Xianhua&SHEN Tianlin**.Advances in effect of environmental stress on ammonia-oxidizing communities[J].Chinese Journal of Applied & Environmental Biology,2021,27(03):1-10.[doi:10.19675/j.cnki.1006-687x.2020.03005]
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[综 述] 环境胁迫对氨氧化菌群的影响研究进展()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
27卷
期数:
2021年03期
页码:
1-10
栏目:
综 述
出版日期:
2021-06-25

文章信息/Info

Title:
Advances in effect of environmental stress on ammonia-oxidizing communities
作者:
安丽荣1 卞文新1 刘宝华2 申天琳1**
1山东农业大学资源与环境学院,土肥资源高效利用国家工程实验室 泰安 271018
2德州市平原县农业农村局 德州 253100
Author(s):
AN Lirong1 BIAN Wenxin1 LIU Xianhua2&SHEN Tianlin1**
1 National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer, College of Resource and Environment, Shandong Agricultural University, Taian, 271018, China
2 Dezhou Bureau of Agriculture and Rural Affairs, Dezhou 253100, China
关键词:
硝化作用氨氧化古菌氨氧化细菌功能基因环境监测
Keywords:
nitrification ammonia-oxidizing bacteria ammonia-oxidizing archaea functional gene environmental monitor
DOI:
10.19675/j.cnki.1006-687x.2020.03005
摘要:
土壤污染物具有隐蔽性、滞后性、难治理性等特点,长期影响着土壤生态环境的安全,如何全面、实时地监测土壤环境质量成为当今的研究热点。氮素转化过程是元素地球化学循环中对土壤污染和质量十分敏感的代谢过程。硝化作用是氮素转化的主要过程之一,本文从硝化作用的限速步骤氨氧化过程为切入点,综述了环境条件如pH、铵浓度、O2浓度、有机质含量、温度、季节等对氨氧化细菌(Ammonia-oxidizing bacteria, AOB)和氨氧化古菌(Ammonia-oxidizing archaea, AOA)的生理生化性质、转录水平和群落结构的影响,以及利用分子生物学方法测定氨氧化菌群群结构和其功能基因表达量用于反映环境变化的应用前景。得出的结论主要为:微生物在酶的协同作用下完成氨氧化作用;目前对氨氧化菌群群落结构的研究对象主要为16S rRNA基因和amoA功能基因;在不同的生态环境条件下,氨氧化古菌比氨氧化细菌的分布更广,且在同一环境因子胁迫下,AOB和AOA的响应有所不同,AOA在低温、低铵浓度、酸性等寡营养条件下更为活跃,AOB则在高铵浓度、碱性等条件下更为活跃,这表明二者在不同的环境条件下对硝化作用的贡献不同。最后对氨氧化菌群群落结构以及氨氧化相关基因表达水平在揭示环境变化方面进行了展望,认为未来对氨氧化微生物代谢关键酶的基因表达信息、AOA和AOB变化规律与环境因子之间的相关性进行深入研究是非常有必要的。(图1 表1 参82)
Abstract:
Soil pollutants affect the safety of soil ecological environment for a long time, because they have the characteristics of concealment, hysteresis quality and difficult to control. After the “Soil Pollution Prevention and Control Action Plan” proposed by the State Council , the current research focus was how to monitor soil environmental quality in a comprehensive and real-time manner. The nitrogen transformation was one of the geochemical cycles of elements, that was sensitive to soil pollution and quality. And nitrification was one of the important processes affecting the nitrogen transformation. The first and rate-limiting step of nitrification was ammonia-oxidizing process. The physiological and biochemical properties, the transcription level and the community structure of ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA) were reviewed under environmental stress such as pH, ammonium availability, O2 concentration, organic matter content, temperature, and seasons. The molecular biology methods to determine the structures of ammonia-oxidizing microbe and the expressions of the functional genes were summed up for their application in reflecting environmental changes. The conclusions were: ammonia oxidation is accomplished under the synergistic action of enzymes; the current research objects on the communities structure of ammonia oxidizing microbe were mainly 16S rRNA gene s and ammonia oxidation function gene s; in different ecological environments, ammonia oxidizing archaea were more widely distributed than ammonia oxidizing bacteria; the responses of ammonia oxidizing archaea and ammonia oxidizing bacteria to the same environmental stress were different (AOA was more active under low temperature, low ammonium concentration, acid and other oligotrophic conditions, while AOB was more active under high ammonium concentration and alkalinity) , which indicated that they had different contributions to nitrification under the environmental conditions. Finally, we concluded the application of ammonia-oxidizing microorganism communities’ structure and ammonia-oxidizing function genes’ transcription level to reveal environmental changes, and the necessity of studying the gene expression of key enzymes in the metabolism of ammonia oxidizing microorganisms and the correlation s between AOA / AOB changes and environmental stress in the future .

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备注/Memo

备注/Memo:

收稿日期 Received: 2020-03-03 接受日期 Accepted: 2020-08-28
国家自然科学基金青年科学基金项目(41703080)、国家重点研发计划项目子课题(2017YFD0200704)、山东省重大科技创新工程项目子课题(2017CXGC0306)和山东省高等学校科技计划项目资助(J16LF01)资助
**通讯作者 Corresponding author (E-mail: tl_shen@163.com)
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更新日期/Last Update: 2020-09-10