|本期目录/Table of Contents|

[1]夏库拉·巴克特亚尔 林青 刘珍珠 杨红梅 娄恺** 李萍.新疆且末地区沙尘暴空气真菌多样性[J].应用与环境生物学报,2021,27(01):1-16.[doi:10.19675/j.cnki.1006-687x.2020.01033]
 Xakula·Bahetiyaer,Lin Qing,Liu Zhenzhu,et al.Diversity of air fungi in sandstorms in Qiemo district, Xinjiang[J].Chinese Journal of Applied & Environmental Biology,2021,27(01):1-16.[doi:10.19675/j.cnki.1006-687x.2020.01033]
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新疆且末地区沙尘暴空气真菌多样性()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
27卷
期数:
2021年01期
页码:
1-16
栏目:
出版日期:
2021-02-25

文章信息/Info

Title:
Diversity of air fungi in sandstorms in Qiemo district, Xinjiang
作者:
夏库拉·巴克特亚尔12 林青1 刘珍珠1 杨红梅1 娄恺1** 李萍3
1新疆农业科学院微生物应用研究所,新疆特殊环境微生物实验室 乌鲁木齐 830091
2新疆大学生命科学与技术学院 乌鲁木齐 830046
3库尔勒市农业技术推广中心 库尔勒 841000
Author(s):
Xakula·Bahetiyaer12 Lin Qing1 Liu Zhenzhu1 Yang Hongmei1 Lou kai1* & Li Ping3
1 Institute of Microbiology, Xinjiang Academy of Agriculture, Urumqi 830091, China
2College of Life Science and Technology, Xinjiang University, Urumqi 830046, China
3Korla Agricultural Technology Extension Center, Korla 841000, China
关键词:
沙尘暴高通量测序空气微生物多样性环境因子
Keywords:
sandstorm high-throughput sequencing air microbial diversity environmental factor
DOI:
10.19675/j.cnki.1006-687x.2020.01033
摘要:
采集新疆且末地区沙尘暴空气微生物样品,揭示沙尘暴前期、中期和后期真菌组成和群落结构差异,为沙尘暴灾害预警提供依据。利用可培养方法和高通量测序技术,分析新疆且末地区沙尘暴空气真菌的组成和物种多样性。结果表明:(1)可培养空气真菌隶属于子囊菌门,其优势菌属为链格孢属(Alternaria,55.56%);(2)高通量测序技术检测的沙尘暴空气样品中共获得555 548条有效序列,Sobs指数在10-100之间,Shannon指数在1.106-2.903之间,Coverage值在0.999-1之间,表明测序数据信息量充足。序列聚类为257个可操作分类单元,包括6个门,121个属。其中,门水平上,优势菌门为子囊菌门(Ascomycota,58.66%)和担子菌门(Basidiomycota,31.03%)。优势菌属为链格孢属(Alternaria,21.86%)、栓菌属(Trametes,20.82%)、耐温酵母(Geuhomyces,20.30%);沙尘暴中期空气真菌多样性>沙尘暴前期空气真菌多样性>沙尘暴后期空气真菌多样性。(3)冗余分析结果显示,湿度、气压、经度与沙尘暴空气真菌多样性呈正相关,与海拔、维度、温度呈负相关。综上所述,沙尘暴蕴藏着丰富的微生物资源,不同的环境因子是影响沙尘暴空气微生物群落结构变化的重要因素,结果可为荒漠化治理和开发微生物新资源提供一定理论依据。
Abstract:
The airbone microbial samples of sandstorms in Qiemo area of Xinjiang were collected to reveal the differences of fungal composition and community structure in the early, middle and late stages of sandstorms, so as to provide basis for early warning of sandstorms. Using cultivable methods and high-throughput sequencing technology, the composition and species diversity of sand dust air fungi in Qiemo area of Xinjiang were analyzed. The results showed that: (1) The culturable air fungi belonged to the Ascomycete phylum, the dominant genus was: Alternaria (55.56%); (2) A total of 555548 effective sequences were obtained from the sandstorm air samples by high-throughput sequencing technology, the Sobs index was between 10-100, the Shannon index was between 1.106-2.903, and the Coverage value was between 0.999-1. These results indicated that the sequencing data has sufficient information. Sequence clustering is 257 operable taxa, including 6 gates and 121 genera. Among them, at the phylum level, the dominant phylum was Ascomycota (Ascomycota, 58.66%) and Basidiomycota (Basidiomycota, 31.03%). The dominant genus were: Alternaria (21.86%), Trametes (20.82%), Geuhomyces (20.30%); the diversity of airborne fungi in the middle stage of sandstorm was higher than that in the early stage of sandstorm > that in the later stage. (3) Redundancy analysis results showed that humidity, air pressure, and longitude are positively correlated with airborne fungal diversity in sandstorms, and negatively correlated with altitude, latitude, and temperature. In summary, sand and dust storms contain rich microbial resources. Different environmental factors are important factors that affect the changes in the microbial community structure of sand and dust storms. Understanding the dust and airborne microorganisms provides a theoretical basis for desertification control and the development of new microbial resources.

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备注/Memo

备注/Memo:
收稿日期 Received: 2020-01-15 接受日期 Accepted: 2020-05-18
国家自然科学基金项目(31660028)资助 Supported by the National Natural Science Foundation of China (31660028)
**通信作者 Corresponding author (E-mail: loukai02@mail.tsinghua.edu.cn)
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更新日期/Last Update: 2020-08-04