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[1]赵春丽 陈家兰 王乐 陈何 郑友峰 肖昉 赖钟雄** 刘生财**.温度对苋菜幼苗中甜菜色素含量及其相关基因表达的影响[J].应用与环境生物学报,2020,26(04):1-8.[doi:10.19675/j.cnki.1006-687x.2019.11038]
 ZHAO Chunli,CHEN Jialan,WANG Le,et al.Effect of temperature on betalain content and related genes expression in amaranth seedlings[J].Chinese Journal of Applied & Environmental Biology,2020,26(04):1-8.[doi:10.19675/j.cnki.1006-687x.2019.11038]
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温度对苋菜幼苗中甜菜色素含量及其相关基因表达的影响()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
26卷
期数:
2020年04期
页码:
1-8
栏目:
研究论文
出版日期:
2020-08-25

文章信息/Info

Title:
Effect of temperature on betalain content and related genes expression in amaranth seedlings
作者:
赵春丽 陈家兰 王乐 陈何 郑友峰 肖昉 赖钟雄** 刘生财**
福建农林大学 园艺植物生物工程研究所 福州 350002
Author(s):
ZHAO Chunli CHEN Jialan WANG Le CHEN He ZHENG Youfeng XIAO Fang LAI Zhongxiong LIU Shengcai
Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
关键词:
温度苋菜甜菜色素基因表达
Keywords:
temperature Amaranthus tricolor L betalain gene expression
DOI:
10.19675/j.cnki.1006-687x.2019.11038
摘要:
温度是影响植物生长发育的重要环境因子,为探究温度对苋菜幼苗生长发育及甜菜色素合成的影响,本以‘大红’苋菜7 d幼苗为试验材料,分析不同温度、不同时间处理下苋菜幼苗形态、甜菜色素含量和甜菜色素合成相关基因表达量的差异。结果显示:(1)形态观察发现,高温(35 ℃、40 ℃)和低温(15 ℃、20 ℃)都会使苋菜幼苗生长势变弱、植株红色变淡,且处理时间越久变化程度越明显,其最适温度范围在25-30 ℃之间。(2)甜菜色素含量测定结果发现,随着温度的升高,甜菜色素含量呈现先上升后下降的趋势,且处理时间越久变化趋势越明显,25 ℃时甜菜色素含量最高,但30 ℃和25 ℃之间没有显著差异。(3)qPCR分析发现,随着温度的升高,AtGAI、AmMYB1、AmCYP76AD1、AmaDODA基因相对表达量大致都呈现先上升后下降的趋势,且处理时间越久变化趋势越明显。上述结果表明:苋菜幼苗生长及甜菜色素合成的最适温度范围为25-30 ℃,且温度可能通过调控AtGAI基因的表达来调控AmMYB1基因和甜菜色素合成基因AmCYP76AD1、AmaDODA的表达,进而影响苋菜幼苗生长发育及甜菜色素合成,为将来苋菜栽培中的环境调控提供理论参考。(图4 表1 参31)
Abstract:
Temperature is an important environmental factor affecting plant growth and development. In order to explore the effect of temperature on the growth and development of amaranth seedlings and the synthesis of betalain. In this study, 7 d seedlings of Amaranthus tricolor L. cv. Dahong has been employed as experimental materials to analyze the differences in seedling morphology, betalain content and expression of genes related to betalain synthesis under treatment of the different temperatures and different time. The results showed that: (1) Morphological observation showed that high temperature ( 35 ℃, 40 ℃) and low temperature (15 ℃, 20 ℃) would make the growth potential of amaranth seedlings weaker, the plant red became lighter, and the longer the treatment time, the more obvious the change. Its optimum temperature range is between 25-30 ℃. (2) The results of betalain content determination showed that the content of betalain increased first and then decreased with the increase of temperature, and the longer the treatment time, the more obvious the change trend. The betalain content was the highest at 25 ℃, but 30 ℃ and 25 ℃ was no significant difference. (3) qRT-PCR analysis showed that the relative expressions of AtGAI, AmMYB1, AmCYP76AD1 and AmaDODA genes increased first and then decreased with the increase of temperature, and the longer the treatment time, the more obvious the trend. The above results indicated that the optimal temperature range for the growth of amaranth seedlings and the synthesis of betalain pigments was 25-30 ℃, and the temperature may regulate the expression of AmMYB1 gene and betalain synthesis genes AmCYP76AD1 and AmaDODA by regulating the expression of AtGAI gene, further affecting the growth and development of amaranth seedlings and the synthesis of betalain, provide a theoretical reference for environmental regulation in the cultivation of A. tricolor L. in the future.

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备注/Memo

备注/Memo:
收稿日期 Received: 2019-11-16 接受日期 Accepted: 2020-01-13
*福建省自然科学基金(2018J01700)、福建农林大学科技创新专项基金项目(CXZX2017174,CXZX2016118,CXZX2017189)、福建省高原学科建设经费(102/71201801101)资助
**通信作者Corresponding authors(E-mail: 1215698900@qq.com; laizx01@163.com)
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更新日期/Last Update: 2020-01-14