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[1]杨凤 张玲利 王东梅 刘燕 周敏 张国林 代玉梅** 罗应刚**.小麦内生菌Pedobacter chitinilyticus CM134L-2 T的次级代谢产物分离与鉴定[J].应用与环境生物学报,2020,26(04):1-7.[doi:10.19675/j.cnki.1006-687x.2019.10017]
 YANG Feng,ZHANG Linli,WANG Dongmei,et al.Isolation and identification of secondary metabolites of endophytic bacteria Pedobacter chitinilyticus CM134L-2 T from wheat[J].Chinese Journal of Applied & Environmental Biology,2020,26(04):1-7.[doi:10.19675/j.cnki.1006-687x.2019.10017]
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小麦内生菌Pedobacter chitinilyticus CM134L-2 T的次级代谢产物分离与鉴定()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
26卷
期数:
2020年04期
页码:
1-7
栏目:
研究论文
出版日期:
2020-08-25

文章信息/Info

Title:
Isolation and identification of secondary metabolites of endophytic bacteria Pedobacter chitinilyticus CM134L-2 T from wheat
作者:
杨凤12 张玲利1 王东梅12 刘燕1 周敏1 张国林1 代玉梅1** 罗应刚1**
1中国科学院成都生物研究所,成都 610041
2中国科学院大学,北京100049
Author(s):
YANG Feng12 ZHANG Linli1 WANG Dongmei12 LIU Yan1 ZHOU Min1 ZHANG Guolin1 DAI Yumei1** & LUO Yinggang1**

1Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China
2University of Chinese Academy of Sciences, Beijing 100049, China
关键词:
内生菌小麦Pedobacter chitinilyticus次级代谢产物
Keywords:
Endophyte wheat Pedobacter chitinilyticus secondary metabolites
DOI:
10.19675/j.cnki.1006-687x.2019.10017
摘要:
小麦是世界上种植最广泛的谷类作物之一。我们前期从对条锈病菌侵染有抗性的小麦叶片中分离得到以几丁质为唯一碳源的内生新菌种Pedobacter chitinilyticus CM134L-2 T。为研究这株小麦内生菌的次级代谢产物,我们对该菌进行了大量发酵,将发酵液进行乙酸乙酯和正丁醇萃取,对萃取物中的化合物进行了分离、纯化。通过波谱分析及与已知化合物数据对比,我们从该菌发酵液中共鉴定了7个化合物,它们分别为:3-羟基乙酰基吲哚(1)和4-羟基-5-(吲哚-3-基)-5-氧代戊烷-2-酮(2)2个吲哚类衍生物,环(L-脯氨酸-L-缬氨酸)(3),苯甲酸(4)、对羟基苯甲酸(5)和邻氨基苯甲酸(6)3个苯甲酸类衍生物,以及硫杆菌素A (7)。P. chitinilyticus CM134L-2 T的上述代谢产物被报道具有调节植物生长、促进根系生长、或抗菌等作用。研究结果为Pedobacter属微生物作为新型的植物益生或生防菌株提供了参考。
Abstract:
A novel endophytic bacteria strain Pedobacter chitinilyticus CM134L-2 T was isolated and identified from wheat, one of the most important cereals in the world, with chitin as the sole carbon source. To investigate the secondary metabolites profiling of this endophyte, the bacteria was grown and fermented. The fermentation broth was extracted with ethyl acetate and n-butanol, respectively. The following separation and purification of the above mentioned extracts offered 7 known natural compounds. They were identified as 3-hydroxyacetyl indole ( 1), 4-hydroxy-5-(indole-3-yl)-5-oxopentane-2-one (2), cyclo(L-proline-L-valine) (3), benzoic acid (4), p-hydroxybenzoic acid ( 5), o-aminobenzoic acid ( 6), and sulfobacin A ( 7) on the basis of spectroscopic data interpretation and comparison their corresponding spectral data with that of the reported data. This study showed that P. chitinilyticus CM134L-2 T has the ability to produce phytohormones, antimicrobial substances and preservatives. It may be of great significance for the research and application of wheat endophytes and their secondary metabolites.

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备注/Memo

备注/Memo:
收稿日期 Received: 2019-10-12 接受日期 Accepted: 2019-11-21
*国家自然科学基金项目(41877042)成都市科技惠民项目(2016-HM01-00380-SF)资助 Supported by the National Natural Science Foundation of China (41877042) and Chengdu Science and Technology Programmes for People’s Benefits (2016-HM01-00380-SF).
**通讯作者 Corresponding authors (E-mail: daiym@cib.ac.cn; yinggluo@cib.ac.cn)
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更新日期/Last Update: 2019-12-05