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[1]李海红 佟欣宇 宦臣臣 闫志英**.高效异养硝化-好氧反硝化菌株TS-1筛选及降解特性[J].应用与环境生物学报,2021,27(02):1-16.[doi:10.19675/j.cnki.1006-687x.2019.10016]
 LI Haihong,TONG Xinyu,HUAN Chenchen & YAN Zhiying **.Isolation and identification of heterotrophic nitrification-aerobic denitrifying strain TS-1 and its nitrogen removal characteristics[J].Chinese Journal of Applied & Environmental Biology,2021,27(02):1-16.[doi:10.19675/j.cnki.1006-687x.2019.10016]
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高效异养硝化-好氧反硝化菌株TS-1筛选及降解特性()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
27卷
期数:
2021年02期
页码:
1-16
栏目:
研究论文
出版日期:
2021-04-25

文章信息/Info

Title:
Isolation and identification of heterotrophic nitrification-aerobic denitrifying strain TS-1 and its nitrogen removal characteristics
作者:
李海红1 佟欣宇1 宦臣臣2 闫志英2**
1西安工程大学 西安 710000
2中国科学院成都生物研究所 成都 6100 41
Author(s):
LI Haihong 1 TONG Xinyu 1 HUAN Chenchen 2 & YAN Zhiying 2**
1 Xian Polytechnic University, Xian 710000, China
2 Chengdu Institute of Biology, Chinese Academy of Sciences , Chengdu 610049, China
关键词:
异养硝化好氧反硝化Paracoccus denitrificans混合氮源生物脱氮
Keywords:
heterotrophic nitrification aerobic denitrification Paracoccus denitrificans mixed nitrogen source biological nitrogen removal
DOI:
10.19675/j.cnki.1006-687x.2019.10016
摘要:
从实验室定向驯化的活性污泥中分离筛选出一株具有异养硝化-好氧反硝化功能的菌株TS-1。通过生理生化及16S rRNA基因序列鉴定其为脱氮副球菌,通过单因素和正交实验对其去除NH4+-N的最佳条件进行优化,并通过对比进一步探究其在不同氮源条件下对各形态无机氮的去除规律。实验表明该菌株最适碳源为丁二酸钠,最佳C/N为15,最佳接种量为5%,最适温度为30 ℃、pH为8.0。以初始浓度约为100 mg/L的NH4+-N、NO3--N和NO2--N分别为单一氮源时,菌株TS-1对各形态氮的去除率为97.49%、100%和95.94%;维持各形态氮初始浓度不变,将其两两混合时发现混合氮源中若包含NO2--N会使菌株OD600值达到最大值所用时间延长,氮源中含有NH4+-N会降低菌株对其他形态氮源的去除率,以及NO3--N的添加会使菌株对NH4+-N的去除能力降低;3种形态氮源同时存在的条件下,该菌对各氮源去除能力由强至弱为NO2--N > NH4+ -N > NO3--N。本研究从活性污泥中分离筛选出一株具有高效异养硝化-好氧反硝化功能的菌株TS-1,通过研究碳源、氮源、温度、pH得到最佳降解条件,可为废水短程脱氮提供参考。(图19 表4 参37)
Abstract:
In this paper, a strain TS-1 with heterotrophic nitrification-aerobic denitrification function was isolated and screened from activated sludge acclimated in a laboratory. According to physiological and biochemical characteristics and 16S rRNA gene sequence, it was identified as Paracoccus denitrificans. Through single factor and orthogonal experiments, the optimal conditions for NH4+-N removal were optimized, and the removal rules of various forms of inorganic nitrogen under different nitrogen sources were further explored through comparison. Experiments show that the optimal carbon source of the strain is sodium succinate, the optimal C/N is 15, the optimal inoculation amount is 5%, the optimal temperature is 30 ℃, and the pH value is 8.0. When NH4+-N, NO3--N and NO2--N with an initial concentration of about 100 mg/L were used as single nitrogen source respectively, the removal rates of TS-1 to each nitrogen source were 97.49%, 100% and 95.94%. The initial concentration of various forms of nitrogen is kept unchanged, and it is found that if NO2--N is contained in the mixed nitrogen source, the time taken for the strain OD 600 to reach the maximum value will be prolonged. The inclusion of NH4+-N will reduce the removal rate of other nitrogen sources by the pressure. And the addition of NO3--N will reduce the NH4+-N removal ability of the stress. Under the condition that three forms of nitrogen sources exist simultaneously, the removal ability of the bacteria to each nitrogen source is NO2--N > NH4+-N > NO3--N from strong to weak. In this study, a strain TS-1 with high efficiency heterotrophic nitrification-aerobic denitrification was isolated from activated sludge. The best degradation conditions were obtained through the study of carbon source, nitrogen source, temperature and pH, hoping to provide reference for short-range denitrification of wastewater.

相似文献/References:

[1]张悦,曾薇,刘春兰,等.单级好氧和限氧污水处理系统中总氮损失的微生物学机理[J].应用与环境生物学报,2009,15(03):399.[doi:10.3724/SP.J.1145.2009.00399]
 ZHANG Yue,ZENG Wei,LIU Chunlan,et al.Microbiological Mechanism of Nitrogen Loss in Aerobic or Oxygen-limited Wastewater Treatment System[J].Chinese Journal of Applied & Environmental Biology,2009,15(02):399.[doi:10.3724/SP.J.1145.2009.00399]
[2]丁炜,朱亮,徐京,等.好氧反硝化菌及其在生物处理与修复中的应用研究进展[J].应用与环境生物学报,2011,17(06):923.[doi:10.3724/SP.J.1145.2011.00923]
 DING Wei,ZHU Liang,XU Jing,et al.Progress of Researches on Aerobic Denitrifiers and Their Application in Bioremediation[J].Chinese Journal of Applied & Environmental Biology,2011,17(02):923.[doi:10.3724/SP.J.1145.2011.00923]
[3]黄廷林,何秀秀,张海涵,等.水库贫营养异养硝化-好氧反硝化菌Sxf14的脱氮特性[J].应用与环境生物学报,2015,21(02):201.[doi:10.3724/SP.J.1145.2014.10002]
 HUANG Tinglin,HE Xiuxiu,ZHANG Haihan,et al.Nitrogen removal characteristics of the heterotrophic nitrification-aerobic denitrification bacterium Acinetobacter sp. Sxf14[J].Chinese Journal of Applied & Environmental Biology,2015,21(02):201.[doi:10.3724/SP.J.1145.2014.10002]
[4]李海红,佟欣宇,宦臣臣,等.高效异养硝化-好氧反硝化菌株TS-1筛选及降解特性[J].应用与环境生物学报,2020,26(04):791.
 LI Haihong,TONG Xinyu,HUAN Chenchen & YAN Zhiying.Characterizing the heterotrophically nitrifying and aerobically denitrifying TS-1 strain[J].Chinese Journal of Applied & Environmental Biology,2020,26(02):791.

备注/Memo

备注/Memo:
收稿日期 Received: 2019-10-12 接受日期 Accepted: 2020-01-14
四川省重大科技专项课题(2018SZDZX0024)和中国科学院战略生物资源计划(KFJ-BRP-009)资助
**通讯作者 Corresponding author (E-mail: yanzy@cib.ac.cn)
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更新日期/Last Update: 2020-07-03