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[1]孙建瑞 赵君峰 符丹丹 王大红.不同光质对衣藻(Chlamydomonas sp. 212 )生长及油脂积累的影响[J].应用与环境生物学报,2020,26(04):1-12.[doi:10.19675/j.cnki.1006-687x.2019.09019]
 SUN Jianrui,ZHAO Junfeng,FU Dandan & WANG Dahong**.Effects of Different Light Quality on the Growth and Lipid Accumulation of Chlamydomonas sp. 212[J].Chinese Journal of Applied & Environmental Biology,2020,26(04):1-12.[doi:10.19675/j.cnki.1006-687x.2019.09019]
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不同光质对衣藻(Chlamydomonas sp. 212 )生长及油脂积累的影响()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
26卷
期数:
2020年04期
页码:
1-12
栏目:
研究论文
出版日期:
2020-08-25

文章信息/Info

Title:
Effects of Different Light Quality on the Growth and Lipid Accumulation of Chlamydomonas sp. 212
作者:
孙建瑞 赵君峰 符丹丹 王大红
河南科技大学食品与生物工程学院 洛阳 471023
Author(s):
SUN Jianrui ZHAO Junfeng FU Dandan & WANG Dahong**
College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China
关键词:
微藻油脂光质生长特性脂肪酸
Keywords:
microalgae lipid light quality growth characteristics fatty acid
DOI:
10.19675/j.cnki.1006-687x.2019.09019
摘要:
研究白光、红光、蓝光、红蓝光(1:3)、红蓝光(1:2)、红蓝光(1:1)、红蓝光(2:1)和红蓝光(3:1)8种光质在100 μmol·m-2·s-1的光照强度下对Chlamydomonas sp. 212 生长特性及油脂积累的影响。在不同光质条件下进行Chlamydomonas sp. 212 的培养,对其生物量、光合色素含量、碳水化合物含量、蛋白质含量、油脂积累量及脂肪酸组成等指标进行测量与综合分析。结果发现,蓝光下Chlamydomonas sp. 212 的生物量达到最大的0.3351 g/L,红光下其生物量最小。蓝光下Chlamydomonas sp. 212 的叶绿素a、叶绿素b和类胡萝卜素的含量均为最高,分别为12.26 mg/L、6.34 mg/L和5.94 mg/L;红光下叶绿素a、叶绿素b和类胡萝卜素的含量均较低。Chlamydomonas sp. 212 在红光下碳水化合物的含量最高27.54%,蛋白质的含量最低23.68%;蓝光下碳水化合物的含量最低19.12%,蛋白质的含量为31.46%;红蓝光下碳水化合物和蛋白质的含量低于白光。Chlamydomonas sp. 212 在蓝光下油脂含量为29.86%,产量为100.06 mg/L;红光下油脂含量为27.52%,油脂产量最低;而红蓝光下油脂的积累量明显升高,红蓝光(1:2)下油脂含量最高35.25%,红蓝光(1:3)下油脂产量最高109.23 mg/L。不同光质对Chlamydomonas sp. 212 脂肪酸组成的影响不显著,C16和C18脂肪酸的相对含量达到93%以上,饱和脂肪酸约占47%,不饱和脂肪酸约占53%。研究表明,蓝光有利于Chlamydomonas sp. 212 的生长,红光不利于其生长;蓝光有利于油脂的积累,红蓝光下油脂的积累显著升高,且蓝光占比越高,越有利于油脂的积累,红蓝光(1:3)下油脂的积累量最高。图6表1参32
Abstract:
Objectives: Effects of white light, red light, blue light, red and blue light (1:3), red and blue light (1:2), red and blue light (1:1), red and blue light (2:1), red and blue light (3:1) under light intensity with 100 μmol·m-2·s-1 on the growth characteristics and lipid accumulation of Chlamydomonas sp. 212 were studied. Methods: Chlamydomonas sp. 212 was cultured under different light quality, and its biomass, photosynthetic pigment content, carbohydrate content, protein content, lipid accumulation and fatty acid composition were measured and comprehensively analyzed. Results: The biomass of Chlamydomonas sp. 212 reached the maximum of 0.3351 g/L under blue light, and minimum under red light. The contents of chlorophyll a, chlorophyll b and carotenoids of Chlamydomonas sp. 212 were the highest under blue light, which were 12.26 mg/L, 6.34 mg/L and 5.94 mg/L, respectively; the contents of chlorophyll a, chlorophyll b and carotenoids were lower under red light. Chlamydomonas sp. 212 had the maximum carbohydrate content of 27.54%, and minimum protein content of 23.68% under red light; while it had the minimum carbohydrate content of 19.12%, and protein content of 31.46% under blue light . Besides, the carbohydrate and protein content under red and blue light were lower than white light. The of Chlamydomonas sp.212 was 29.86% and the production was 100.06 mg/L under blue light; while the lipid content was 27.52% and the production was lowest under red light. It was worth noting that lipid accumulation was significantly increased under red and blue light. The lipid content was the highest 35.25% under red and blue light (1:2) , and the lipid production was the highest 109.23 mg/L under red and blue light (1:3). The effects of different light quality on the fatty acid composition were not significant, and the relative content of C16 and C18 fatty acids was more than 93%, while the relative content of saturated fatty acids was about 47%, unsaturated fatty acids about 53%. Conclusions: Blue light was conducive to the growth of Chlamydomonas sp. 212, but red light was not. Blue light was beneficial to the lipid accumulation, and the lipid accumulation was significantly increased under red and blue light . Moreover, the higher the proportion of blue light, the more conducive to the lipid accumulation, and lipid production was the highest under red and blue light (1:3).

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备注/Memo

备注/Memo:
收稿日期 Received: 2019-09-09 接受日期 Accepted: 2019-11-17
*国家自然科学基金项目(31401672)、河南省高等学校重点科研项目(17B180003)资助 Supported by the National Natural Science Foundation of China (No. 31401672), Key Scientific Research Projects of Henan Province (No. 17B180003)
**通讯作者 Corresponding author (E-mail: wangdahong2003@163.com)
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更新日期/Last Update: 2019-12-05