|本期目录/Table of Contents|

[1]项丽慧 陈林** 余文权 张应根.茶树GH1基因家族鉴定及其在茶鲜叶萎凋过程的表达[J].应用与环境生物学报,2020,26(03):1-14.[doi:10.19675/j.cnki.1006-687x.2019.08040]
 XIANG Lihui,CHEN Lin**,YU Wenquan & ZHANG Yinggen.Identification of GH1 gene family of Camellia sinensis and expression analysis during the withering process of fresh tea leaves *[J].Chinese Journal of Applied & Environmental Biology,2020,26(03):1-14.[doi:10.19675/j.cnki.1006-687x.2019.08040]
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茶树GH1基因家族鉴定及其在茶鲜叶萎凋过程的表达()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
26卷
期数:
2020年03期
页码:
1-14
栏目:
研究论文
出版日期:
2020-06-25

文章信息/Info

Title:
Identification of GH1 gene family of Camellia sinensis and expression analysis during the withering process of fresh tea leaves *
作者:
项丽慧1 陈林1** 余文权2 张应根1
1 福建省农业科学院茶叶研究所 福州 35 0011
2 福建省农业科学院 福州 350002
Author(s):
XIANG Lihui1 CHEN Lin1** YU Wenquan2 & ZHANG Yinggen 1
1. Tea Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 35 0011, China;
2. Fujian Academy of Agricultural Sciences, Fuzhou 350002, China
关键词:
茶树糖苷水解酶第1家族萎凋表达分析
Keywords:
Camellia sinensis Glycoside Hydrolase family 1 withering expression analysis
DOI:
10.19675/j.cnki.1006-687x.2019.08040
摘要:
糖苷水解酶第1家族基因(GH1)在茶叶香气形成中发挥着重要作用,为了解茶树GH1家族成员的进化特性及其在萎凋过程的表达规律,基于茶树基因组数据库,本研究对GH1基因家族进行全基因组鉴定,并对其基因结构、编码蛋白的理化性质、亚细胞定位、保守基序及其在不同组织和茶鲜叶萎凋过程的表达模式进行分析。结果显示,茶树GH1基因家族共31个成员,分为5个亚家族,同一亚家族的GH1具有类似的保守基序,保守性较高。GH1基因编码142-871个氨基酸,GH1在细胞中分布很广,包括分泌通路、细胞核、胞浆等。GH1基因表达量从高到低依次为幼嫩茎段,幼根,幼果,成熟叶,嫩叶,顶芽,老叶和花。大部分GH1基因在鲜叶和萎凋减重10%阶段优势表达,而CsGH1BG6,CsGH1BG7和CsGH1BG26在茶鲜叶萎凋减重20%至60%之间上调表达,这是GH1基因参与白茶加工品质调控的关键时期。本研究表明,上调表达的GH1基因对茶鲜叶的失水胁迫调控和萎凋过程香气的形成起到积极的作用,可作为进一步开展茶鲜叶萎凋过程品质形成研究的候选基因。茶树GH1基因家族的鉴定及其在不同组织和茶鲜叶萎凋过程中的表达分析为茶叶加工品质调控提供理论基础。(图5 表2 参30 )
Abstract:
Glycoside Hydrolase family 1 genes ( GH1) played a vital role in the formation of tea aroma. This paper aimed to understand the evolutionary characteristics of GH1 family members and their expression during the withering of fresh tea leaves. Based on the tea genome database, the GH1 gene family members were identified. Additionally, the gene structure, physicochemical properties, subcellular localizations and conserved motifs of their encoded proteins and the expression patterns in different tissues and the withering process were analyzed. In total, 31 GH1 gene family members were identified in 5 subgroups, and the same subfamily genes had similar conservative motifs and were highly conserved. These GH1 genes encoded proteins containing 142-871 amino acids, and GH1 were widely distributed in cells, including secretory, nucleus, cytosol and so on. The expression levels of GH1 genes from high to low were immature stems, tender roots, young fruits, mature leaves, young leaves, apical buds, old leaves and flowers. Most of the GH1 genes were dominantly expressed in the fresh leaves and 10% reduction in leaves weight samples, while CsGH1BG6, CsGH1BG7 and CsGH1BG26 were up-re gulated at the weight loss was 20% to 60%, which wa s a key period for the GH1 gene to participate in the regulation of white tea processing quality. Up-regulated GH1 genes played a positive role in the regulation of water-deficient stress and the formation of aroma during the withering of fresh tea leaves, and they could be used as candidate genes for further research on the quality formation of the withering process. Identification of the GH1 gene family and expression analysis in different tissues and the withering process of tea leaves could provide a theoretical basis for the quality control of tea manufacture.

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备注/Memo

备注/Memo:
收稿日期 Received: 2019-08-26 接受日期 Accepted: 2019-10-21
*福建省属公益类科研院所基本科研专项(2018R1012-5;2019R1029-5)、福建省科技重大专项(2017NZ0002-1)、福建省农业科学院科技创新团队(STIT2017-1-3)资助
**通讯作者 Corresponding author (E-mail: chenlin_xy@163.com)
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更新日期/Last Update: 2019-12-04