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[1]项蕾蕾 李丹 孙雪丽 田娜 刘范 付帅 陈裕坤 林玉玲 程春振 王天池** 赖钟雄**.LED补光对香蕉组培苗增殖和生理生化指标的影响[J].应用与环境生物学报,2020,26(03):1-11.[doi:10.19675/j.cnki.1006-687x.2019.08038]
 XIANG Leilei,LI Dan,SUN Xueli,et al.Effects of LED supplementation on Proliferation, physiological and biochemical Indexes of tissue cultured banana plantlets[J].Chinese Journal of Applied & Environmental Biology,2020,26(03):1-11.[doi:10.19675/j.cnki.1006-687x.2019.08038]
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LED补光对香蕉组培苗增殖和生理生化指标的影响()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
26卷
期数:
2020年03期
页码:
1-11
栏目:
研究论文
出版日期:
2020-06-25

文章信息/Info

Title:
Effects of LED supplementation on Proliferation, physiological and biochemical Indexes of tissue cultured banana plantlets
作者:
项蕾蕾 李丹 孙雪丽 田娜 刘范 付帅 陈裕坤 林玉玲 程春振 王天池** 赖钟雄**
福建农林大学园艺学院/园艺植物生物工程研究所 福州 350002
Author(s):
XIANG Leilei LI Dan SUN Xueli TIAN Na LIU Fan FU Shuai CHEN Yukun LIN Yuling CHENG Chunzhen WANG Tianchi** & LAI Zhongxiong**
College of Horticulture/Institute of Horticultural Biotechnology,Fujian Agriculture and Forestry University,Fuzhou 350002,China
关键词:
香蕉LED补光增殖系数生理生化指标叶绿素含量叶绿素荧光
Keywords:
banana LED supplementation proliferation coefficient physiology and biochemistry indexes chlorophyll content chlorophyll fluorescence
DOI:
10.19675/j.cnki.1006-687x.2019.08038
摘要:
为研究补充不同光质的LED光源对香蕉组培苗增殖和相关生理生化指标的影响,本研究以‘天宝蕉’为材料,通过设置普通荧光+LED红光(R)、普通荧光+LED蓝光(B)、普通荧光+LED绿光(G)、普通荧光+LED白光(W)、普通荧光+LED暖白光(WW)、普通荧光+LED暖黄光(WY),对照组普通荧光7种处理,比较了不同LED补光对香蕉组培苗的增殖率、相关生理指标、叶绿素含量及叶绿素荧光参数的影响。结果显示,WY处理下香蕉组培苗的增殖系数最大,B处理下香蕉苗的增殖系数最小;R处理下,植株高度最大,B处理下,植株假茎最粗,WW处理下,有最大的根长,W处理下根数、根粗、叶数、叶宽、叶长的数值最大,同时鲜重和含水量最高,壮苗系数最优。与CK相比,R、B和G处理会降低叶绿素以及类胡萝卜素含量,而补充LED白光可以提高叶绿素以及类胡萝卜素含量。此外,LED补光,尤其是R、W和G补光会引起香蕉组培苗叶片Fv/Fo、Fv/Fm和NPQ参数的显著增加和Y(Ⅱ)、ETR、和qP等参数的降低。研究结果表明,在增殖培养阶段补充LED暖黄光,香蕉组培苗的增殖率最高;在生根培养阶段补充LED白光,香蕉组培苗的叶绿素含量、chla/chlb比值、类胡萝卜素含量最大,同时光合效率和壮苗系数最高。本研究为香蕉组培苗增殖和生根阶段的光质选择奠定基础。
Abstract:
Objectives: In order to study the effects of LED light supplementation on the proliferation, physiological and biochemical indexes of tissue cultured banana plantlets. Methods: By using ’Tianbaojiao’ banana as experimental material, seven different LED treatments, respectively with ordinary fluorescence plus LED red light (R), ordinary fluorescence plus LED blue light (B), ordinary fluorescence plus LED green light (G), ordinary fluorescence plus LED white light (W), ordinary fluorescence plus LED warm white light (WW), ordinary fluorescence plus LED warm yellow (WY), and ordinary fluorescence (CK), were performed to compare the effects of LED supplementation on the growth rate, related physiological indexes, chlorophyll content and chlorophyll fluorescence parameters of banana plantlets. Result: The multiplication coefficient of banana plantlets was found to be the highest under WY treatment and the smallest under B treatment. The largest height was found under R treatment, the largest pseudostem diameter under B treatment, the largest root length was found under WW treatment. The largest values of maximum root length, root number, root diameter, leaf number, leaf width and length were found under W treatment. Moreover, compared with CK, R, B and G treatments showed inhibition on chlorophyll and carotenoid accumulation, but white light supplementation could increase chlorophyll content and carotenoid content, and plants in this group showed the highest fresh weight, water content and optimum seedling coefficient. Furthermore, R and G treatments with monochromatic light could decrease the content of chlorophyll and carotenoid, while white LED supplementation could increase the content of chlorophyll and carotenoid. In addition, supplemental LED light, especially red, white and green light could significantly increase the parameters of Fv/Fo, Fv/Fm and NPQ, but inhibit the Y( Ⅱ), ETR and qP parameters. Conclusions: During multiplication culture process, warm yellow light supplementation could get the highest plantlet growth rate. During rooting, white light LED supplementation is helpful for the chlorophyll and carotenoid accumulations and the highest chla/chlb ratio, photosynthetic efficiency and seedling strengthening coefficient could be obtained under this treatment. Our study can provide basis for the future LED light supplementation in the banana tissue culture.

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备注/Memo

备注/Memo:
收稿日期 Received: 2019-08-25 接受日期 Accepted: 2019-10-21
*国家自然科学基金项目(31601713),国家现代农业产业技术体系专项资金(CARS-31-15),科技创新专项基金(CXZX2018076),科技创新专项基金(CXZX2017189)
*通讯作者 Author for correspondence (E-mail: Wangtchi@hortmail.com, E-mail: laizx01@163.com)
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更新日期/Last Update: 2019-12-04