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[1]张伶俐** 孙毅 陈锦 王新峰**.产甲烷菌抑制剂对便秘小鼠结肠微生物菌群及代谢产物的影响[J].应用与环境生物学报,2020,26(03):1-9.[doi:10.19675/j.cnki.1006-687x.2019.07005]
 ZHANG Lingli **,SUN Yi,CHEN Jin & WANG Xinfeng**.Effects of methanogens inhibitor on the composition of gut microbiota and short-chain fatty acids in mice with constipation[J].Chinese Journal of Applied & Environmental Biology,2020,26(03):1-9.[doi:10.19675/j.cnki.1006-687x.2019.07005]
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产甲烷菌抑制剂对便秘小鼠结肠微生物菌群及代谢产物的影响()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
26卷
期数:
2020年03期
页码:
1-9
栏目:
研究论文
出版日期:
2020-06-25

文章信息/Info

Title:
Effects of methanogens inhibitor on the composition of gut microbiota and short-chain fatty acids in mice with constipation
作者:
张伶俐1** 孙毅1 陈锦1 王新峰2**
1汕头大学医学院实验动物中心 汕头 515041
2石河子大学动物科技学院 石河子 832000
Author(s):
ZHANG Lingli 1** SUN Yi1 CHEN Jin1 & WANG Xinfeng2**
1Department of Experimental Animal Center Shantou University Medical College, Shantou 515041, China
2College of Animal Science and Technology, Shihezi University, Shihezi 832000 , China
关键词:
产甲烷菌抑制剂便秘肠道微生物短链脂肪酸
Keywords:
methanogens inhibitor constipation gut microbiota short-chain fatty acids
DOI:
10.19675/j.cnki.1006-687x.2019.07005
摘要:
为研究产甲烷菌抑制剂(溴氯甲烷)对便秘小鼠的肠道微生物菌群及代谢产物的影响,选取60只5周龄雄性KM小鼠,随机分为3组:对照组、便秘组、甲烷抑制剂干预组。试验结束,采集小鼠结肠内容物通过高通量测序技术分析肠道菌群;气相色谱分析结肠内容物中短链脂肪酸含量;real-time PCR技术分析肠道总菌及产甲烷菌数量变化。结果表明溴氯甲烷处理后小鼠粪便颗粒数及粪便含水率显著增加,结肠内容物Lactobacillus及Streptococcus的相对丰度显著增加,Lachnospiraceae NK4a136 group ,Lachnospiraceae_uncultured,Bacterodies,Muribaculaceae_norank及Staphylococcus的相对丰度显著降低,结肠菌群结构发生显著变化,产甲烷菌数量显著降低,但对总菌数量无显著影响。溴氯甲烷处理组总短链脂肪酸含量显著降低。综上表明,便秘小鼠在甲烷抑制剂干预后对结肠菌群结构及代谢产物均有显著影响。
Abstract:
The aim of this study was to evaluate the effects of methanogens inhibitor ( Bromochloromethane(BCM)) on the composition of gut microbiota and short-chain fatty acids in the mice with constipation induced by loperamide. Sixty Kunming mice were randomly divided into the follow three groups: normal control, constipation model, BCM group. At the end of experiment, the colonic microbiota was analyzed by pyrosequencing of the 16S rRNA gens and real-time PCR, and metabolites were profiled by gas chromatography. The results showed that BCM significantly change altered the bacterial community composition. BCM treatment significantly increased the dejection number and the water content of the feces. BCM treatment increased the abundance of Lactobacillus and Streptococcus, while decreased the abundance of Lachnospiraceae NK4a136 group , Lachnospiraceae_uncultured, Bacterodies, Muribaculaceae_norank and Staphylococcus in the colonic content . BCM decreased the abundance of methanogen populations and did not affect the total colonic bacterial counts. BCM treatment decreased the concentration of short chain fatty acids. In conclusion, BCM treatment significantly altered the microbiotic and metabolite profiles in the colonic digest of mice with constipation.

备注/Memo

备注/Memo:
收稿日期 Received: 2019-07-03 接受日期 Accepted: 2019-10-14
*广东省创新强校工程项目青年创新人才项目(2018KQNCX083)和国家自然科学基金项目(31360558)资助
**通讯作者Corresponding author (E-mail: zhangll_007@163.com; wxf-4@163.com)
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更新日期/Last Update: 2019-12-04