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[1]孙悦 胡渤洋 徐鑫 刘子璐 李小凤 张国庆,**.二年残孔菌的培养条件优化及其液体发酵产物的抗氧化及漆酶活性*[J].应用与环境生物学报,2020,26(02):1-10.[doi:10.19675/j.cnki.1006-687x.2019.05039]
 SUN Yue,HU Boyang,XU Xin,et al.Culture condition optimization, antioxidant capacity, and laccase production of Abortiporus biennis*[J].Chinese Journal of Applied & Environmental Biology,2020,26(02):1-10.[doi:10.19675/j.cnki.1006-687x.2019.05039]
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二年残孔菌的培养条件优化及其液体发酵产物的抗氧化及漆酶活性*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
26卷
期数:
2020年02期
页码:
1-10
栏目:
研究论文
出版日期:
2020-03-20

文章信息/Info

Title:
Culture condition optimization, antioxidant capacity, and laccase production of Abortiporus biennis*
作者:
孙悦1 胡渤洋1 徐鑫1 刘子璐1 李小凤1 张国庆1 2**
1北京农学院生物与资源环境学院,农业农村部华北都市农业重点实验室 北京 102206
2北京农学院植物科学技术学院,北京林木分子设计育种高精尖创新中心,农业应用新技术北京市重点实验室 北京 102206
Author(s):
SUN Yue1 HU Boyang1 XU Xin1 LIU Zilu1 LI Xiaofeng1 ZHANG Guoqing1 2 **
1 College of Bioscience and Resource Environment, Beijing University of Agriculture, Key Laboratory of Urban Agriculture (North China) of Ministry of Agriculture and Rural Affairs, Beijing 102206, China
2 College of Plant Science and Technology, Beijing University of Agriculture, Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Key Laboratory for Agricultural Application and New Technique, Beijing 102206, China
关键词:
二年残孔菌培养条件抗氧化活性漆酶
Keywords:
Abortiporus biennis culture conditions antioxidant activity laccase
DOI:
10.19675/j.cnki.1006-687x.2019.05039
摘要:
二年残孔菌Abortiporus biennis)是一种具有抗氧化抗肿瘤功效的药用真菌,可降解木质素。本二年残孔菌WX-02Cu2+诱导产漆酶活性研究。基于单因子实验的最适生长条件结果表明,葡萄糖是WX-02菌株菌丝体生长的最适碳源,牛肉膏最适氮源C/N比为40/1玉米粉最适生长因子32 ℃。10%(V/V)接种量28 ℃、150 rmin培养72 hPD培养基发酵液的总抗氧化活性为2.76 ± 0.3L(FeSO4)和0.40 ± 0.01 L(Trolox),DPPH自由基清除率为100.00 ± 0.00%总SOD活力为15.7 ± 0.25 U/mL;最适培养条件液体培养基发酵液的总抗氧化活性为0.20 ± 0.0L(FeSO4)和0.1 ± 0.01 mmol/L(Trolox),DPPH自由基清除率为59.06 ± 0.61%,总SOD活力为15.6 ± 0.62 U/mL。0.25 mmol/L Cu2+对胞外漆酶诱导效果最佳,PD培养基、0.25 mmol/L Cu2+、28 ℃、150 rpm培养96 h为× 105 U/mL,是对照组的1.72倍WX-02菌株具有良好的抗氧化和产漆酶活性,具有药用和环境治理开发应用潜能。(图4 表4 参34)
Abstract:
Abortiporus biennis is a medicinal white-rot fungus with antioxidation and anti-tumor effects, which can degrade lignin and cause white decay of wood. In the present study, A. biennis strain WX-02 isolated from Wuxi, Jiangsu Province, was used as the material. Optimal culture conditions of mycelia, antioxidant activities, and Cu2+ induced laccase production of liquid fermentation were further investigated. Optimum culture conditions based on single factor experiments have revealed that the optimum carbon source for strain WX-02 mycelial growth was glucose, while the optimum nitrogen sources were beef extract and soybean powder. When the C/N ratio was 40/1, the mycelia demonstrates the greatest growth vigor. The optimum growth factors were determined to be corn flour and vitamin C, while the optimal pH and temperature were 7.0 and 32 ℃, respectively. With the culture conditions of a 10% (V/V) inoculation amount at 28 ℃ and 150 r/min for 72 h, the fermentation broth using PD medium manifested a total antioxidant activity of 2.76 ± 0.31 mmol/L (FeSO4) and 0.40 ± 0.01 mmol/L (Trolox), DPPH radical scavenging ratio of 100.00 ± 0.00%, and SOD activity of 15.75 ± 0.25 U/mL. Whereas the fermentation broth using the optimum culture condition medium demonstrated a total antioxidant activity of 0.20 ± 0.03 mmol/L (FeSO4) and 0.16 ± 0.01 mmol/L (Trolox), DPPH radical scavenging ratio of 59.06 ± 0.61%, and SOD activity of 15.65 ± 0.62 U/mL. Further study of Cu2+ on extracellular laccase production revealed that Cu2+ of 0.25 mmol/L can significantly enhanced the enzyme production with the highest activity of (1.82 ± 0.02) × 105 U/mL after the liquid fermentation at 28 ℃ at 150 r/min for 96 h, which was 1.72 times as much as the control group. The strain WX-02 demonstrated considerable high antioxidant and laccase activities, suggesting its potential applications in medicinal and environmental management.

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备注/Memo

备注/Memo:
收稿日期 Received: 2019-05-23 接受日期 Accepted: 2019-07-09
*北京市自然科学基金项目(5162006)、北京高等学校高水平人才交叉培养计划项目(PXM2019_014207_000035)和北京农学院内涵发展定额大学生科研训练项目(kx20190XX)资助
**通讯作者 Corresponding author (E-mail: zhanggqbua@163.com)
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更新日期/Last Update: 2019-07-30