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[1]李冠军 刘鑫铭 梁安洁 林勇明** 李键**.两株木麻黄内生真菌代谢产物对NaCl胁迫的响应*[J].应用与环境生物学报,2020,26(01):1-10.[doi:10.19675/j.cnki.1006-687x.2019.05034]
 LI Guanjun,LIU Xinming,LIANG Anjie,et al.Effects of NaCl stress on metabolites of two endophytic fungi of Casuarina equisetifolia*[J].Chinese Journal of Applied & Environmental Biology,2020,26(01):1-10.[doi:10.19675/j.cnki.1006-687x.2019.05034]
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两株木麻黄内生真菌代谢产物对NaCl胁迫的响应*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
26卷
期数:
2020年01期
页码:
1-10
栏目:
研究论文
出版日期:
2020-01-25

文章信息/Info

Title:
Effects of NaCl stress on metabolites of two endophytic fungi of Casuarina equisetifolia*
作者:
李冠军12 刘鑫铭12 梁安洁12 林勇明12** 李键2**
1福建农林大学林学院 福州 350002
2福建省高校森林生态系统过程与经营重点实验室 福州 350002
Author(s):
LI Guanjun12 LIU Xinming12 LIANG Anjie12 LIN Yongming12** & LI Jian12**
1 College of Forestry,Fujian Agriculture and Forestry University, Fuzhou 350002, China
2 Key Laboratory of Forest ecosystem process and Management of Fujian Province, Fuzhou 350002, China
关键词:
内生真菌盐胁迫代谢产物植物激素
Keywords:
Endophyte salt stress metabolite plant hormone
DOI:
10.19675/j.cnki.1006-687x.2019.05034
摘要:
为明晰抗盐木麻黄内生真菌次生代谢物与盐分胁迫之间的关系,选用高效液相色谱仪(HPLC)和气相色谱-质谱联用仪(GC-MS)对不同NaCl浓度培养的两株木麻黄内生真菌(Y6、Z1)及其混菌发酵液进行了检测并分析比较。结果显示:(1)Y6菌发酵液在5%NaCl浓度下赤霉素含量最大,与其它处理有显著差异(P < 0.05),Z1菌发酵液中玉米素含量显著高于Y6菌和混菌发酵液中玉米素含量,0%浓度下,混菌发酵液吲哚-3-乙酸含量最大,与其它处理有显著差异(P < 0.05),Y6菌和Z1菌发酵液中吲哚-3-乙酸含量最大值分别在1%和3%浓度。(2)两菌株发酵液成分相对含量高于30%的物质主要有环(L-脯氨酰-L-亮氨酰)、喇叭茶醇、5-亚硝基-2,4,6-三氨基嘧啶、2-丁基-1-辛醇、邻苯二甲酸二正辛酯、5-羟甲基糠醛,高浓度(5%)环境下产出的物质种类显著不同于低浓度(0%)环境下的物质。上述研究表明,两株内生真菌及其混菌均能产生吲哚-3-乙酸、赤霉素和玉米素三种植物激素,不同内生真菌、不同NaCl浓度菌株发酵液成分存在差异,高浓度(5%)环境下能够诱导两株内生真菌产生丰富的代谢产物,但上述物质是否都对木麻黄幼苗生长具有抗盐、促生和抑菌效果以及其作用机理还有待进一步研究。(图1表3参43)
Abstract:
In order to study the relationship between salt stress and secondary metabolites of endophytic fungi of Casuarina equisetifolia, the metabolites of two endophytic fungi (Y6, Z1) and their mixed strains of Casuarina equisetifolia grown in different salinity were detected by high performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS). Y6 strain fermentation broth has the highest gibberellin content at 5% salinity, significantly different from other treatments (P < 0.05), the zeatin content in the fermentation broth of Z1 strain was significantly higher than that in the fermentation broth of Y6 strain and mixed strain. At 0% salinity, the content of indole-3-acetic acid in the fermentation broth of the mixed strain was the highest, significantly different from other treatments (P < 0.05), the maximum content of indole-3-acetic acid in the fermentation broth of Y6 strain and Z1 strain was 1% and 3% salinity, respectively. Secondary metabolites with a relative content of more than 30% mainly include 3-Isobutylhexahydropyrrolo[1,2-a]pyrazine-1,4-dione; 1,1,4,7-Tetramethyldecahydro-1H-cyclopropa[e]azulen-4-ol; 2-butyl-1-octanol; 5-Nitroso-2,4,6-pyrimidinetriamine; 1,2-Benzenedicarboxylic acid and 5-Hyddroxymethylfurfurole, the secondary metabolite species of endophytic fungi grown in high salt (5%) environment were different from the secondary metabolite species of endophytic fungi grown in low salt (0%) environment. The results of this study demonstrated that both endophytic fungi and their mixed strains can produce three plant hormones, indole-3-acetic acid, gibberellin and zein, endophytic fungi species and growing environment (different salinity) can affect endophytic fungal secondary metabolites, endophytic fungi grown under high salt (5%) environment can produce a variety of secondary metabolites, however, whether the above substances have anti-salt, growth-promoting and antibacterial effects on the growth of Casuarina equisetifolia seedlings and its mechanism remains to be further studied.

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备注/Memo

备注/Memo:
收稿日期 Received: 2019-05-21 接受日期 Acceptted: 2019-06-19
*国家自然科学基金项目(31400533)、福建农林大学科技创新专项基金(CXZX2018122)和福建农林大学林学院林学高峰学科优秀博硕士培养工程项目(71201800781)资助
**共同通讯作者Joint corresponding authors (E-mail: hmilycau@163.commonkey1422@163.com)
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更新日期/Last Update: 2019-06-19