|本期目录/Table of Contents|

[1]侯颖** 裴韬 王维宇 尤晓颜 牛明福 黄天晨.一株氰氟草酯降解新菌株Sphingopyxis sp.WP9的分离鉴定及降解特性*[J].应用与环境生物学报,2019,25(06):1-10.[doi:10.19675/j.cnki.1006-687x.2019.03005]
 HOU Ying**,PEI Tao,WANG Weiyu,et al.Isolation and Identification of New Cyhalofop-butyl (CyB)-degrading Strain Sphingopyxis sp. WP9 and Its Degradation Characteristics *[J].Chinese Journal of Applied & Environmental Biology,2019,25(06):1-10.[doi:10.19675/j.cnki.1006-687x.2019.03005]
点击复制

一株氰氟草酯降解新菌株Sphingopyxis sp.WP9的分离鉴定及降解特性*()
分享到:

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年06期
页码:
1-10
栏目:
研究论文
出版日期:
2019-12-30

文章信息/Info

Title:
Isolation and Identification of New Cyhalofop-butyl (CyB)-degrading Strain Sphingopyxis sp. WP9 and Its Degradation Characteristics *
作者:
侯颖** 裴韬 王维宇 尤晓颜 牛明福 黄天晨
河南科技大学食品与生物工程学院 洛阳 471023
Author(s):
HOU Ying** PEI Tao WANG Weiyu YOU Xiaoyan NIU Mingfu & HUANG Tianchen
College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China
关键词:
氰氟草酯鞘氨醇盒菌分离鉴定降解特性
Keywords:
cyhalofop-butyl Sphingopyxis isolation and identification degradation characteristics
DOI:
10.19675/j.cnki.1006-687x.2019.03005
摘要:
氰氟草酯是应用于稻田的一种除草剂,其广泛使用给水生生态带来了一定威胁。利用连续富集培养的方法,从农药厂活性污泥中,筛选出一株氰氟草酯高效降解菌WP9。采用16S rRNA基因序列分析并结合菌落菌体形态和生理生化特性,将菌株WP9鉴定为鞘氨醇盒菌(Sphingopyxis)。在以100 mg/L氰氟草酯为唯一碳源的无机盐培养基中,菌株WP9在120 h内可将其降解97.03%。菌株WP9降解炔草酯的最适温度和pH分别为30 ℃和7.0;当菌株WP9的接种量≥5%时,其对100 mg/L氰氟草酯的降解效果较好;当氰氟草酯浓度≤100 mg/L时,菌株WP9对氰氟草酯的平均降解速率随着底物浓度的增加而呈指数增长。通过UPLC-MS鉴定降解产物为氰氟草酸和氰氟草酚,推测氰氟草酯首先通过酯键水解生成氰氟草酸,然后氰氟草酸通过醚键水解生成氰氟草酚。本研究是首次报道鞘氨醇盒菌对氰氟草酯的降解,可为开展氰氟草酯污染环境的生物修复提供新菌种和理论基础。(图8 参30)
Abstract:
Cyhalofop-butyl (CyB) is a kind of herbicide applied in rice paddy fields, but its widespread application poses a threat for the aquatic ecology. An efficient CyB-degrading strain WP9 was screened from the active sludge of a pesticide factory by continuous enrichment and cultivation. Strain WP9 was identified as Sphingopyxis by combining the 16S rRNA gene sequencing with morphological observation, physiological and biochemical characteristics testing. Strain WP9 could degrade 97.03% of 100 mg/L CyB in the inorganic salt medium, in which CyB used as the sole carbon source. The optimum temperature and pH for the degradation of CyB by strain WP9 were 37 ℃ and 8.0, respectively. When the concentration of CyB ≤ 100 mg/L, the average degradation rate of CyB by strain WP9 increased exponentially with the increase of the substrate concentration. When the inoculation size of strain WP9 ≥ 5%, the degradation of 100 mg/L CyB by strain WP9 was efficient. The motebolites of cyhalofop-butyl were identified as cyhalofop acid (CyA) and cyhalofop phenol by UPLC-MS. The possible metabolic pathway for CyB is proposed as: CyB was transformed to CyA quickly by hydrolyzing of the ester bond, and then CyA was converted to cyhalofop phenol. This study is the first report of CyB biodegradation by Sphingopyxis and it provide a new strain and theoretical basis for the bioremediation of environment polluted by CyB.

相似文献/References:

[1]聂志娟,顾谢军,蔡舒,等.一株除草剂氰氟草酯降解菌的分离鉴定与降解特性[J].应用与环境生物学报,2011,17(06):869.[doi:10.3724/SP.J.1145.2011.00869]
 NIE Zhijuan,GU Xiejun,CAI Shu,et al.Isolation of a Cyhalofop-butyl-degrading Bacterium and Its Degradation Characteristics[J].Chinese Journal of Applied & Environmental Biology,2011,17(06):869.[doi:10.3724/SP.J.1145.2011.00869]

备注/Memo

备注/Memo:
收稿日期 Received: 2019-03-02 接受日期 Accepted: 2019-03-27
*国家自然科学基金项目(31400098)、河南省科技攻关计划项目(172102310435)、河南科技大学人才基金项目(09001642)和河南科技大学科研创新能力培育基金项目(2014ZCX025)资助
**通讯作者 Corresponding author (E-mail: houying76@126.com
点击摘要页题目后的“PDF”可下载阅读全文;本文为已录用的作者修定稿,尚未经编辑全面修改。
引用本文请注明出处本刊;发表刊期和页码将以正式出版时的安排为准,但DOI确定不变。
更新日期/Last Update: 2019-03-28