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[1]罗川 任丽平** 张智 聂煜东.植物激素与底物浓度对小球藻生长及油脂积累的影响*[J].应用与环境生物学报,2019,25(06):1-7.[doi:10.19675/j.cnki.1006-687x.2019.02036]
 LUO Chuan,REN Lipin **,ZHANG Zhi,et al.Effects of phytohormones and substrate concentration on Chlorella vulgaris growth and lipid accumulation*[J].Chinese Journal of Applied & Environmental Biology,2019,25(06):1-7.[doi:10.19675/j.cnki.1006-687x.2019.02036]
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植物激素与底物浓度对小球藻生长及油脂积累的影响*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年06期
页码:
1-7
栏目:
研究论文
出版日期:
2019-12-30

文章信息/Info

Title:
Effects of phytohormones and substrate concentration on Chlorella vulgaris growth and lipid accumulation*
作者:
罗川1 任丽平1** 张智2 聂煜东3
1西华师范大学环境科学与工程学院 南充 637009 ;
2重庆大学城市建设与环境工程学院 重庆 400045;
3重庆工商大学废油资源化技术与装备教育部工程研究中心 重庆 400060
Author(s):
LUO Chuan1 REN Lipin 1** ZHANG Zhi2 NIE Yudong3
1College of Environmental Science and Engineering, China West Normal University , Nanchong 637009, China
2College of Urban Construction and Environmental Engineering, Chongqing University , Chongqing 401331, China
3Engineering Research Center for Waste Oil Recovery Technology and Equipment of Education, Chongqing Technology and Business University, Chongqing 400060, China
关键词:
小球藻生物柴油植物激素油脂产率生态-经济效益分析
Keywords:
Chlorella vulgari s Biodiesel Phytohormone Lipid yield Ecological-economic efficiency analysis
DOI:
10.19675/j.cnki.1006-687x.2019.02036
摘要:
利用植物激素提高微藻生长速率及油脂合成速率是降低微藻生物柴油开发成本的有效手段之一。对比不同浓度天然植物激素吲哚-3-乙酸(IAA)和人工合成类激素1-萘乙酸(NAA)作为微藻培养添加剂对小球藻(Chlorella vulgaris)生长及脂质积累的影响,以BG11培养基作为培养底物,探讨不同底物浓度(10%、25%、50%、75%、100%)对植物激素施用效果的影响。结果显示,两种植物激素对小球藻生长及脂质合成影响显著(p < 0.05 ),IAA的促生长效果优于NAA,最佳施用浓度均为2 mg/L,培养15 d后所获生物量为(525.87 ± 5.9 1)mg/L 、(367.72 ± 4.1) mg/L ,分别是对照组生物量的2.48倍和1.7倍;IAA和NAA浓度与脂质积累呈线性相关,IAA在0.05-2 mg/L时表现为促进中性脂积累,NAA在1-5 mg/L时表现为促油脂积累;2 mg/L IAA 和2 mg/LNAA诱导后的小球藻生物质产率及油脂产率最高,分别为(35.06 ± 0.39 ) 、(24.52 ± 0.27 ) mg/ (L·d),(21.96 ± 0.62) 、(14.38 ± 0.09 ) mg/ (L·d),与未加植物激素对照组相比,分别提高了59.68%、41.23%、74.99%、61.78%;小球藻在不同底物浓度中生长及脂质积累差异显著(p < 0.05 ),但对植物激素施用效果影响不明显。综上,植物激素对微藻培养体系影响巨大,低浓度IAA与NAA的施用均有助于提升培养效率,且满足生态效益的要求,但从经济效益角度考虑,2 mg/L NAA更适宜作为小球藻养殖的植物激素及添加剂量。(图3表2参35)
Abstract:
Objectives: The use of phytohormones to i ncrease the growth rate of microalgae and the rate of oil synthesis are one of the effective approach to decrease the cost of microalgae biodiesel development. Methods: Comparative study on the effects of different concentrations of natural Phytohormone Indole-3-acetic acid (IAA) and synthetic hormone 1-Naphthaleneacetic acid (NAA) as microalgae culture additives on the growth and lipid accumulation of Chlorella vulgaris. Using BG11 medium as culture substrate, were compared the effects of different substrate concentrations (10%, 25%, 50%, 75%, 100%) on the application of phytohormone were studied in the present work. Results: The results showed that the two plant hormones had significant effects on the growth and lipid synthesis of C. vulgaris (p < 0.05). The dose of 2 mg/L IAA had a better growth promoting effect than 2 mg/L NAA. After 15 d culture, the biomass obtained was (525.87 ± 5.91) mg/L and (367.72 ± 4.1) mg/L respectively, which were 2.48-fold and 1.7-fold higher than that of the control groups; The concentrations of IAA and NAA were linearly correlated with lipid accumulation. IAA showed a neutral lipid accumulation at 0.05-2 mg/L while NAA showed lipid accumulation at 1-5 mg/L; The biomass yield and oil yield of C. vulgaris were the highest after incuction by 2 mg/L IAA and 2 mg/L NAA with the figure of (35.06 ± 0.39) and (24.52 ± 0.27) mg/(L·d), (21.96 ± 0.62) and (14.38 ± 0.09) mg/(L·d) resceptively. Comparing with the control group, these figures increased by 59.68%, 41.23%, 74.99%, and 61.78%, respectively and the accumulation of C.vulgaris growth and lipid were significantly increased in different substrate concentrations, the difference was significant (p < 0.05), but the effect on the application of phytohormone was not significant. Conclusions: This study shows that phytohormones have a great influence on the microalgae culture system. The application of low concentration IAA and NAA can help to improve the efficiency of the culture system, and meet the requirements of eco-efficiency. However, from the perspective of economic efficiency, 2 mg/L NAA is more suitable as a phytohormone additive for C. vulgari culture.

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备注/Memo

备注/Memo:
收稿日期 Received: 2019-02-28 接受日期 Accepted: 2019-06-03
*国家自然科学基金项目(41606142)、重庆市教委科学技术研究项目(KJ1600620)、重庆市科委的基础研究与前沿探索项目(csts2018jcyjAX0734)、西华师范大学英才基金项目(17YC548)资助
**通讯作者Corresponding author (E-mail:517103575@qq.com)
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更新日期/Last Update: 2019-06-19