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[1]刘子璐 孙悦 张国庆** 陈青君 徐 鑫 胡渤洋 关体坤 李晓祯.高含量漆酶食用菌菌渣的筛选及其染料脱色作用*[J].应用与环境生物学报,2019,25(06):1-12.[doi:10.19675/j.cnki.1006-687x.2019.02031]
 LIU Zilu,SUN Yue,ZHANG Guoqing**,et al.Screening of spent mushroom substrates with high laccase activity and their decolorization of dyes *[J].Chinese Journal of Applied & Environmental Biology,2019,25(06):1-12.[doi:10.19675/j.cnki.1006-687x.2019.02031]
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高含量漆酶食用菌菌渣的筛选及其染料脱色作用*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年06期
页码:
1-12
栏目:
研究论文
出版日期:
2019-12-30

文章信息/Info

Title:
Screening of spent mushroom substrates with high laccase activity and their decolorization of dyes *
作者:
刘子璐1 孙悦1 张国庆1** 陈青君2 徐 鑫1 胡渤洋1 关体坤1 李晓祯1
1北京农学院生物科学与工程学院,农业农村部华北都市农业重点实验室 北京 102206
2北京农学院植物科学技术学院 北京 102206
Author(s):
LIU Zilu1 SUN Yue1 ZHANG Guoqing1** CHEN Qingjun2 XU xin1 HU Boyang1 GUAN tikun1 & LI Xiaozhen 1
1 College of Biological Science and Engineering, Beijing University of Agriculture, Key Laboratory of Urban Agriculture (North China) of Ministry of Agriculture and Rural Affairs , Beijing 102206, China
2 Plant science and technology college, Beijing University of Agriculture, Beijing 102206, China
关键词:
食用菌菌渣杏鲍菇漆酶染料脱色
Keywords:
spent mushroom substrate Pleurotus eryngii laccase dye decolourization
DOI:
10.19675/j.cnki.1006-687x.2019.02031
摘要:
食用菌菌渣(Spent mushroom substrate,SMS)指食用菌出菇后的培养基废弃物,富含菌体蛋白、生物酶、多糖等营养物质,是潜在的生物质资源,用于肥料、饲料、生物修复等领域。我国是全球第一大食用菌生产国,大量菌渣资源一直没有得到科学有效地利用,并造成环境污染。以工厂化、实验室和农业栽培的杏鲍菇、平菇等9种常见食用菌菌渣为材料,进行高产漆酶菌渣筛选,并进一步利用高活性菌渣,对孔雀石绿、活性艳蓝、甲基橙、活性红和活性黑5种染料进行脱色脱毒研究。结果显示,新鲜工厂化杏鲍菇菌渣漆酶活性为7.55 ×104 U/g 。其不同高度菌渣柱对染料脱色的结果显示,15 cm高菌渣脱色效果最佳,对5种染料的脱色率分别为100%、98.60%、72.82%、61.97%和43.73%;全波长光谱扫描显示,孔雀石绿、活性艳蓝经处理后流出液的染料最大光吸收峰显著降低甚至消失。进一步利用其菌渣漆酶粗提物进行染料脱色研究,30 °C孵育24 h脱色率分别为81.24%、49.43%、37.62%、26.34%和13.54%。以大肠杆菌和金黄色葡萄球菌为靶标,对酶促染料降解产物进行了毒性研究,结果显示产物毒性均低于未处理染料。本研究表明工厂化杏鲍菇菌渣具有高漆酶活性,能用于孔雀石绿、活性艳蓝等染料废水的脱色、脱毒,具有重要经济和生态价值。(图4 表4 参39)
Abstract:
Spent mushroom substrate (SMS) is the co-product referring to the cultural medi a after mushroom harvest. It is rich in nutrients such as mycelia proteins, enzymes, and polysaccharides, and used as potential bioresources in fertilizer, feed, bioremediation and other fields. China is the world’s largest producer of edible mushrooms, while a large amount of microbial resources have not been used scientifically and effectively, and cause environmental pollution. In the present study, 9 kinds of SMS were collected from factory, laboratory, and agricultural cultivation modes, such as Pleurotus eryngii, Pleurotus ostreatus, etc. Substrates with high laccase activity were screened, followed by decolorization and detoxification assays toward 5 kinds of dyes including malachite green (MG), r eactive brilliant blue (RBB), m ethyl orange (MO), r eactive red (RR), and r eactive black (RB). The results showed that SMS of P. eryngii by industrialized cultivation demonstrated the highest laccase activity of 7.55 × 10 4 IU/g. Decolorization activity using P. erngii–factory substrate (PEFS) of different height column revealed that a column of 15 cm high possessed the highest decolorization ratios towards the five dyes of 100%, 98.60%, 72.82%, 61.97%, and 43.73%, respectively. Analyzed by full-wavelength spectral scanning, the maximum light absorption of MG and RBB was significantly reduced or even disappeared. Furthermore, crude enzyme extract of PEFS manifested the decolorization ratios of 81.24%, 49.43%, 37.62%, 26.34%, and 13.54%, respectively towards the five dyes after 24 h incubation at 30 °C. Toxicity tests of the enzymatic degradation products of dyes were performed using Escherichia coli and Staphylococcus aureus as targets. The results showed that the products were low-toxicity. In summary, PEFS manifests high laccase activity, and can be applied in decolorization and detoxification of dye wastewater such as MG and RBB. It possesses great potential in economic and ecological applications.

相似文献/References:

[1]李强,陈诚,熊川,等.杏鲍菇转录组数据SSR位点的生物信息学分析[J].应用与环境生物学报,2017,23(03):454.[doi:2016.05014]
 LI Qiang,CHEN Cheng,XIONG Chuan,et al.Bioinformatic analysis of simple sequence repeat (SSR) loci in the Pleurotus eryngii transcriptome[J].Chinese Journal of Applied & Environmental Biology,2017,23(06):454.[doi:2016.05014]

备注/Memo

备注/Memo:
收稿日期 Received: 2019-02-26 接受日期 Accepted: 2019-04-16
*北京市自然科学基金项目(5162006)、农业农村部华北都市农业重点实验室开放课题(kf2017012)和北京农学院本科生科研训练项目(kx2018001)资助
**通讯作者 Corresponding author (E-mail: zhanggqbua@163.com)
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更新日期/Last Update: 2019-04-16