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[1]孙传伯 李姚碧琪 马洋山 赵群**.基于ITS序列分析霍山石斛及其近缘物种之间的亲缘关系[J].应用与环境生物学报,2021,27(02):1-10.[doi:10.19675/j.cnki.1006-687x.2019.02024]
 SUN Chuanbo,LI Yaobiqi,MA Yangshan& ZHAO Qun**,et al.Interspecific relationship among Dendrobium huoshanense and its related species based on ITS sequence[J].Chinese Journal of Applied & Environmental Biology,2021,27(02):1-10.[doi:10.19675/j.cnki.1006-687x.2019.02024]
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基于ITS序列分析霍山石斛及其近缘物种之间的亲缘关系()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
27卷
期数:
2021年02期
页码:
1-10
栏目:
研究论文
出版日期:
2021-04-25

文章信息/Info

Title:
Interspecific relationship among Dendrobium huoshanense and its related species based on ITS sequence
作者:
孙传伯1 李姚碧琪12 马洋山1 赵群1**
1皖西学院生物与制药工程学院 六安 237000
2安徽中医药大学 合肥 230000
Author(s):
SUN Chuanbo1 LI Yaobiqi 1 2 MA Yangshan1& ZHAO Qun1**
1 College of Biological and Pharmaceutical Engineering, West Anhui University, Liuan 237000, China
2 Anhui University of Chinese Medicine , Hefei 230000, China
关键词:
霍山石斛石斛属近缘物种ITS序列 亲缘关系
Keywords:
Dendrobium huoshanense Dendrobium related species ITS relationship
DOI:
10.19675/j.cnki.1006-687x.2019.02024
摘要:
通过分析部分石斛属植物的ITS序列,研究霍山石斛与其近缘物种之间的亲缘关系,并分析石斛属的起源及分化时间。从GenBank下载了1种Malaxis属、1种Liparis属的ITS核苷酸序列为外群,27种石斛属的ITS核序列为内群。经比对后序列长度为682bp,外群及石斛属的A + T含量均高于C + G,其中序列中有416个变异位点。A + T平均含量为46.4%,C + G平均含量为53.6%。从系统发育树中看出内群分为6支,霍山石斛与石斛组中其他物种的亲缘关系最近,与顶叶组的亲缘关系较近,而与Spatulata组、Dendrocoryne组和Latouria组的亲缘关系最远。对石斛属系统发育的分析显示大部分石斛属分组与传统石斛分组相同,与传统石斛分组不同的是顶叶组的鼓槌石斛、叉唇组和瘦轴组的物种都聚类到石斛组,顶叶组小黄花石斛与聚石斛可能应该另立新的石斛组。通过贝叶斯法估算得出石斛属的最近共同祖先时间为21.410 9 Ma,而石斛组的最近共同祖先时间为16.552 2 Ma,组内平均分化时间约为7.344 5 Ma。基于上述结果表明,石斛组起源于中新世早期,组内分化主要发生在中新世后期,气候的改变可能促使了石斛组内分化。
Abstract:
Based on the analysis of the ITS sequences from some Dendrobium plants , the interspecific relationship among Dendrobium huoshanense and its related species was studied. The origin of Dendrobium and divergency of time between Dendrobium huoshanense and other species were also studied. The ITS nucleotide sequences of 1 species from Malaxis and 1 species from Liparis were downloaded from GenBank as the outgroup, and those of 27 species from Dendrobium were the ingroup. After comparison, the length of the sequence was 682bp. The A + T content of outgroup and Dendrobium was higher than that of C + G. There were 416 variation sites in the sequence . The average content of A + T and C + G percentages were 46.4% and 53.6%, respectively. From the phylogenetic tree, the ingroup was divided into 6 clades. D. huoshanense had the nearest relationship with other Dendrobium species and the closer relationship with Chrysotoxae, while it had the furthest relationship with Spatulata, Dendrocoryne, and Latouria, respectively. The phylogeny analysis of Dendrobium showed that grouping of the majority of Dendrobium are the same as grouping of traditional Dendrobium. Unlike the traditional groupings, D. chrysotoxum, Stuposa, and Breviflores were clustered in section Dendrobium. D. jenkinsii and D. lindley probably added into members of a new Dendrobium genus. Using the Bayesian method, the most recent common ancestor (TMRCA) of Dendrobium was estimated about 21.4109 Ma. Section Dendrobium evolved and diversified from an Upper Miocene ( 16.5522 Ma) and a Lower Miocene, respectively ( 7.3445 Ma). The results above indicated that the Dendrobium Group originated in the early Miocene, and the divergency within the groups mainly occurred in the late Miocene. Climate change may have contributed to the division within the Dendrobium groups.

备注/Memo

备注/Memo:
收稿日期 Received: 2020-02-21 接受日期 Accepted: 2020-06-28
国家自然科学基金面上项目(81872955)、安徽高校自然科学研究重点项目(KJ2017A403)、安徽省优秀青年人才计划项目(gxyq2018061)和安徽高校自然科学研究重大项目(KJ2016SD61)资助
**通讯作者 Corresponding author (E-mail: zhaoqun626@163.com)
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更新日期/Last Update: 2020-07-03