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[1]吴玲**.富营养化太湖沉积物中微生物群落及对环境因子的响应*[J].应用与环境生物学报,2019,25(06):1-12.[doi:10.19675/j.cnki.1006-687x.2019.02007]
　WU Ling,**.Response of microbial community structure in eutrophic Taihu sediments to environmental factors*[J].Chinese Journal of Applied & Environmental Biology,2019,25(06):1-12.[doi:10.19675/j.cnki.1006-687x.2019.02007]
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富营养化太湖沉积物中微生物群落及对环境因子的响应*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年06期
页码:
1-12
栏目:
研究论文
出版日期:
2019-12-30

文章信息/Info

Title:
Response of microbial community structure in eutrophic Taihu sediments to environmental factors*
作者:
吴玲1; 2**
1常州工程职业技术学院制药与环境工程学院 常州 213161
2南京师范大学地理科学学院 江苏省物质循环与污染控制重点实验室 南京 210023
Author(s):
WU Ling1;  2**
1 Institute of Pharmaceutical and Environmental Engineering, Changzhou Vocational Institute of Engineering, Changzhou 213164, China
2 Jiangsu Provincial Key Laboratory of Materials Cycling and Pollution Control, School of Geography Sciences, Nanjing Normal University, Nanjing 210023, China
关键词:
微生物多样性; 宏基因组测序技术; 富营养太湖; 沉积物; 环境因子
Keywords:
microbial diversity;  metagenomic sequencing technology ;  eutrophic Taihu Lake ;  sediment;  environmental factors
DOI:
10.19675/j.cnki.1006-687x.2019.02007
摘要:
沉积物是水生态系统重要的组成部分，研究沉积物中的微生物多样性及群落结构有助于从侧面了解水体的水质状况。通过采集太湖中具有不同富营养化水平的梅梁湾（ML）与湖心区（HX）表层沉积物（0~-2cm）为研究对象，测定沉积物样品中的总有机碳（TOC）、总氮（TN）、无机氮（NH4+-N与NO3--N）、pH、氧化还原电位（Eh）与溶解氧（DO），利用基于16S rRNA基因的Illumina Miseq宏基因组测序技术研究沉积物样品中的微生物群落结构，并分析沉积物中微生物群落结构与环境因子的潜在关系。结果显示：太湖两采样点6个沉积物样品中共获得234 408条有效序列，Sobs指数在1811-2442之间，Shannon指数在6.16-6.49之间，Coverage值在96.2%-97.7%之间，说明序列信息量足够大且微生物多样性较高。沉积物中细菌相对丰度为99%以上，共检测到48个门，118个纲。其中变形菌门（proteobacteria）为优势菌门，在ML和HX沉积物中的相对丰度分别为40.5%和35.4%，主要包括δ-proteobacteria和β-proteobacteria纲。其它较为丰富的门类包括绿弯菌门（Chloroflexi）、硝化螺菌门（Nitrospirae）和拟杆菌门（Bacteroidetes）等。将沉积物中纲水平微生物群落组成与理化因子进行Spearman相关分析可知， Eh、DO、pH、NH4+-N和TOC含量能显著影响纲水平优势微生物类群（OUT > 1%）并以Eh影响的微生物类群种类最多。上述研究表明，太湖沉积物中微生物资源丰富，不同富营养湖区理化因子是影响其中微生物群落结构的重要因素。
Abstract:
s The sediment is the important component of the aquatic ecosystem, and the study of microbial diversity in sediments is an important way to evaluate the water quality. This paper aimed to study the microbial community structure in the sediments from areas of Taihu Lake (China) with contrasting degrees of eutrophication, and the effect of main physicochemical indexes on the community structures. The surface sediments (0 ~ - 2 cm) were collected from MeiLiang Bay (ML) and Huxin (HX ) of Taihu Lake, and the total organic carbon (TOC), total nitrogen (TN) and inorganic nitrogen (NH4+-N and NO3--N), pH, redox potential (Eh) and dissolved oxygen (DO) were investigated. Moreover, Illumina Miseq metagenomic sequencing technology was applied to disclose the microbial community structure in different sediment samples, and the potential relationship between microbial community structure and physicochemical factors was also studied. Total 234 408 valid reads from 6 sediments were obtained, with Sobs index of 1811-2442, Shannon index of 6.16-6.49 , and coverage of 96.2%-97.7%. These suggested the sufficient sequence data and the high community diversity. The relative abundance of bacteria in the sediments from sampling areas of Taihu Lake contributed to more than 99% of total sequences, with a total of 48 phyla and 113 classes . Total reads in 6 sediments were mainly affiliated with proteobacteria, which were dominated by two classes of δ-proteobacteria and β-proteobacteria, and the relative proportions of proteobacteria in ML and HX were 40.5% and 35.4%, respectively . Other abundant groups include phylum Chloroflexi, Nitrospirae, Bacteroidetes and so on. Eh, DO, pH, ammonium nitrogen, and TOC values can significantly affect microbial groups on class level (OUT >1%), among which Eh is the factor affecting the largest number of microbial groups based on the Spearman correlation analysis of microbial community structures on class level and physiochemical indexes of sediments. Studies exhibited that there are abundant microbial resources in sediments of Taihu Lake , and physiochemical indexes of different eutrophic areas are the important factors for the microbial community structure.

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备注/Memo

备注/Memo:
收稿日期 Received: 2019-02-09 接受日期 Accepted: 2019-03-27
*国家自然科学基金项目（41771286、41271255）、院级科研基金项目、博士启动基金项目资助 Supported by the National Natural Science Foundation of China ( 41771286 and 41271255), and the Science Foundation and Doctoral Foundation of Colleges
**通讯作者 Corresponding author (E-mail: lwu@czie.edu.cn)
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更新日期/Last Update: 2019-03-29