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[1]孙志鹏 蒲擎宇 尚鹏程 曾小珂 詹秀文 王 刚.云南红豆杉活性成分10-DAB累积分布规律*[J].应用与环境生物学报,2019,25(05):1-13.[doi:10.19675/j.cnki.1006-687x.2019.01006]
 SUN Zhi-peng,Pu Qing- yu,SHANG Peng-cheng,et al.Cumulative distribution regularities of the active component 10-DAB of Taxus yunnanensis*[J].Chinese Journal of Applied & Environmental Biology,2019,25(05):1-13.[doi:10.19675/j.cnki.1006-687x.2019.01006]
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云南红豆杉活性成分10-DAB累积分布规律*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年05期
页码:
1-13
栏目:
研究论文
出版日期:
2019-08-25

文章信息/Info

Title:
Cumulative distribution regularities of the active component 10-DAB of Taxus yunnanensis*
文章编号:
201901006
作者:
孙志鹏 蒲擎宇 尚鹏程 曾小珂 詹秀文 王 刚
四川农业大学林学院 四川都江堰 611830
Author(s):
SUN Zhi-peng Pu Qing- yu SHANG Peng-cheng ZENG Xiao-keZHAN Xiu-wen & Wang Gang**
College of Forestry, Sichuan Agricultural University, Dujiangyan Sichuan 611830
关键词:
云南红豆杉HPLC10-DAB累积生长时期海拔
Keywords:
Taxus yunnanensis HPLC 10-DAB accumulation growth stage altitude
DOI:
10.19675/j.cnki.1006-687x.2019.01006
摘要:
为研究云南红豆杉10-DAB的累积规律及单株10-DAB累积量,我们采用HPLC法测定不同海拔、不同生长时期、不同采收期的云南红豆杉枝叶中10-DAB含量,同时测定枝叶生物量。结果显示:红豆杉中10-DAB累积受季节影响显著,从4月份到10月份呈现逐渐递增趋势,10月份10-DAB累积达到最高,之后呈下降趋势;生长年限为2年时,其 10-DAB 累积最高,且与3、4、5年相比具有显著差异(P<0.05),之后随生长年限增加10-DAB累积逐年下降;10-DAB累积在海拔(900±50) m以下无显著性差异(P>0.05),高于(900±50) m后随海拔增加而降低,且与(500±50) m 相比有极显著性差异(P<0.01);生长年限相较于海拔,权重占比更大,对10-DAB的累积影响更大;从单株10-DAB累积量来看,海拔高于900m,单株枝叶10-DAB累积量随海拔升高而呈下降趋势;3年生单株枝叶10-DAB累积量大于2年生、4年生和5年生,单株10-DAB累积量最高可达0.697±0.026 g。本研究表明生长年限、季节、海拔、生物量均显著影响云南红豆杉单株10-DAB累积量,适宜种植云南红豆杉10-DAB药用原料林的最佳海拔范围应在500-900 m,最佳采收生长年限为第3-4年,采收季节10月份。(图6 表2 参33)
Abstract:
Objectives: In order to study the accumulation regularity of 10-DAB from Taxus yunnanensis as well as the cumulated amount of 10-DAB of single plant.Methods: We use HPLC (High Performance Liquid Chromatography) method to measure the content of 10-DAB in the branches and leaves of Taxus yunnanensis at different altitudes, different growth stages and different harvest periods, at the same time, we measure the biomass of leaves and branches. Results: The result shows: the accumulation of 10-DAB in Taxus yunnanensis was significantly influenced by the seasons, from April to October, it showed a gradually increasing trend, while the accumulation of 10-DAB reached the maximum in October, and then showed a down trend; when the growth number of year is two years old, the 10-DAB accumulation is the highest, which is significantly different from that of 3, 4 and 5 years, after that, with the increase of the growth number of year, the accumulation of 10-DAB comes down every year. There is no significant difference in the accumulation of 10-DAB below the altitude (900±50) m, above the (900±50) m, it decreases with the increase of altitude, what is more, compared with (500±50), there is an extremely significant difference; compared with altitude, the growth number of year has a larger proportion of weight and has a greater influence on 10-DAB; from the perspective of 10-DAB cumulated amount of a single plant, the altitude is higher than 900m, and 10-DAB cumulated amount of a single plant branches and leaves shows a down trend with the elevation of the altitude; the cumulated amount of 10-DAB in three years old single plant branches and leaves is larger than two years old, four years old and five years old. The highest 10-DAB cumulated amount of a single plant is 0.697±0.026 g.Conclusions: This study indicates that the growth number of year, season, altitude and biomass all significantly influence the 10-DAB cumulated amount single plant of Taxus yunnanensis, the optimum altitude range for the plantation of 10-DAB Taxus yunnanensis of medicinal material plantation shall be 500- 900m, the optimum harvest growth number of year shall be the 3rd to 4th year, and the harvesting season shall be October.? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?

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备注/Memo

备注/Memo:
收稿日期: 2019-01-04 接受日期 Accepted: 2019-01-29*四川省重点研发项目(2018NZ0057)资助 **通讯作者(E-mail:13908000519@qq.com)点击摘要页题目后的“PDF”可下载阅读全文;本文为已录用的作者修定稿,尚未经编辑全面修改。引用本文请注明出处本刊;发表刊期和页码将以正式出版时的安排为准,但DOI确定不变。
更新日期/Last Update: 2019-02-01