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[1]陈青 王红妹** 李晓红 尉鸿飞 苏朋 何健** 蒋建东.氯乙酰胺类除草剂微生物降解研究进展*[J].应用与环境生物学报,2019,25(05):1-12.[doi:10.19675/j.cnki.1006-687x.2018.12056]
 CHEN Qing,WANG Hongmei**,et al.Research progress in microbial degradation of Chloroacetanilide herbicides*[J].Chinese Journal of Applied & Environmental Biology,2019,25(05):1-12.[doi:10.19675/j.cnki.1006-687x.2018.12056]
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氯乙酰胺类除草剂微生物降解研究进展*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年05期
页码:
1-12
栏目:
综 述
出版日期:
2019-08-25

文章信息/Info

Title:
Research progress in microbial degradation of Chloroacetanilide herbicides*
作者:
陈青13 王红妹1** 李晓红1 尉鸿飞1 苏朋1 何健2** 蒋建东2
1枣庄学院生命科学学院 枣庄 277100
2南京农业大学农业部农业环境微生物生物工程重点实验室 南京 210095
3南京农业大学资源与环境科学学院 南京 210095
Author(s):
CHEN Qing1 3 WANG Hongmei1** LI Xiaohong1 WEI Hongfei1 SU Peng1 HE Jian2** & JIANG Jiandong2
1 College of Life Science, Zaozhuang University , Zaozhuang 277100, China
2 Key Laboratory of Microbiological Engineering Agricultural Environment, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China
3 College of Environmental and Resource Sciences, Nanjing Agricultural University, Nanjing 210095, China
关键词:
氯乙酰胺类除草剂微生物降解生物修复
Keywords:
Chloroacetanilide herbicides Biodegradation Bioremediation
DOI:
10.19675/j.cnki.1006-687x.2018.12056
摘要:
氯乙酰胺类除草剂是酰胺类除草剂一个重要分支,主要分子结构由苯环和氯乙酰胺基团组成,在世界范围内的产量和使用量仅次于草甘膦和磺酰脲类除草剂。随着该类除草剂的大量使用,其在环境中残留量不断增加,已经对生态环境和人类健康造成了严重威胁。该类除草剂在环境中的消解主要是通过微生物代谢实现的,本文从微生物降解菌株资源、降解途径、关键基因和酶等几个方面综述了国内外微生物降解该类除草剂的最新研究进展。细菌主要采用厌氧谷胱甘肽-共轭脱氯和好氧脱烷基-苯环开环代谢途径降解该类除草剂;真菌降解该类除草剂的途径多样化且比较复杂,尚需进一步研究。从细菌中克隆出N-脱烷基酶、酰胺酶和羟化酶基因,但是对于上述酶学特性研究不够深入。同时指出,目前有关微生物降解该类除草剂的研究主要集中于甲草胺、乙草胺和丁草胺,对于异丙草胺和异丙甲草胺微生物降解的代谢途径和分子机制方面的研究仍较少,在今后的工作中要加强异丙草胺和异丙甲草胺微生物降解方面的研究。此外,微生物降解该类除草剂的新型基因为抗除草剂作物新品种的构建提供了良好的基因资源。
Abstract:
Chloroacetanilide herbicides are a class of highly efficient preemergence herbicides that are widely used in the world. Presently, the production and use of the chloroacetanilide herbicides was less than glyphosate and sulfonylurea herbicides. Due to their widespread use, long persistence, and high-water solubility, some of these herbicides have frequently been detected in environment and show threat to human. Microbial metabolism is the most important factor in the degradation of chloroacetanilide herbicides in the environment. In the manuscript, we summarized the latest research progress on the strain’s isolation, identification, biodegradation pathway and molecular mechanisms (such as key genes and enzymes) of these herbicides . The anaerobic-bacteria degradation of the pathway is glutathione-acetanilide conjugates which can be further converted to sulfonated metabolites; The aerobic-bacteria degradation of the pathway is mainly initiated by N/C-dealkylation events, followed by aromatic ring hydroxylation and cleavage proceeds. The fungal degradation pathways of chloroacetanilide herbicides are more complex, therefore further studies are needed. Though, the genes of N-dealkylase, amidase and hydroxylase have been cloned, the characteristics of these enzymes have not been thoroughly studied. Currently, the research has only focused on biodegradation of alachlor, acetochlor and butachlor, but the metabolic pathways and molecular mechanisms for biodegradation of propisochlor and metolachlor have progressed slowly. So, in the future, we should strengthen the research on biodegradation of propisochlor and metolachlor. Especially, study on the chiral selectivity of the microorganisms and enzyme to the isomers of metolachlor. In addition, new genes for microbial degradation of this herbicide may provide gene resources for transgenic crop.

相似文献/References:

[1]李文忠,张鸿翼,杨惠芳.脂肪腈化物的微生物降解[J].应用与环境生物学报,1995,1(01):44.
 Li Wenzhong,Zhang Hongyi,Yang Huifang.MICROBIAL DEGRADATION OF ALIPHATIC NITRILE[J].Chinese Journal of Applied & Environmental Biology,1995,1(05):44.
[2]顾宗濂,谢思琴,周德智,等.城市生活垃圾中酞酸酯的微生物降解[J].应用与环境生物学报,1995,1(03):244.
 Gu Zonglian,Xie Siqin,Zhou Dezhi,et al.MICROBIAL DEGRADATION OF PHTHALATES IN THE MUNICIPAL GARBAGE[J].Chinese Journal of Applied & Environmental Biology,1995,1(05):244.
[3]聂志娟,顾谢军,蔡舒,等.一株除草剂氰氟草酯降解菌的分离鉴定与降解特性[J].应用与环境生物学报,2011,17(06):869.[doi:10.3724/SP.J.1145.2011.00869]
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[4]吴限,高会,周世瑶,等.海洋沉积物中六溴环十二烷的微生物降解特性及降解菌的分离鉴定[J].应用与环境生物学报,2014,20(06):1070.[doi:10.3724/SP.J.1145.2014.03019]
 WU Xian,GAO hui,ZHOU Shiyao,et al.Characteristics of hexabromocyclododecane (HBCDs) degradation by marine sediment microbes and the isolation and identification of degradation bacteria[J].Chinese Journal of Applied & Environmental Biology,2014,20(05):1070.[doi:10.3724/SP.J.1145.2014.03019]
[5]费莹莹,曾献春,杜为军.煤炭污染区土壤中苯并(a)芘降解菌的分离鉴定[J].应用与环境生物学报,2015,21(06):1049.[doi:10.3724/SP.J.1145.2015.07013]
 FEI Yingying,ZENG Xianchun,DU Weijun.Isolation and identification of Benzo(a)pyrene-degrading strains in coal-polluted soil[J].Chinese Journal of Applied & Environmental Biology,2015,21(05):1049.[doi:10.3724/SP.J.1145.2015.07013]

备注/Memo

备注/Memo:
收稿日期 Received: 2018-12-27 接受日期 Accepted: 2019-03-14
*国家自然科学基金项目(31600080)和山东省中青年科学发展基金(ZR2016CB29)资助
**通讯作者 Corresponding author (E-mail: zzxywhm@163.com/hejian@njau.edu.cn )
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更新日期/Last Update: 2019-03-15