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[1]刘生财# 潘君飞# 王 晓 赵春丽 赖钟雄 张梓浩.MeJA对苋菜悬浮细胞类黄酮和类胡萝卜素累积及其代谢相关基因表达的影响[J].应用与环境生物学报,2019,25(04):1-5.
 Effects of methyl jasmonate on the contents and relate metabolic genes of flavonoids and carotenoids in suspension cells of Amaranthus tricolor L..CLC S636.4[J].Chinese Journal of Applied & Environmental Biology,2019,25(04):1-5.
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MeJA对苋菜悬浮细胞类黄酮和类胡萝卜素累积及其代谢相关基因表达的影响()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年04期
页码:
1-5
栏目:
出版日期:
2019-08-31

文章信息/Info

Title:
CLC S636.4
作者:
刘生财# 潘君飞# 王 晓 赵春丽 赖钟雄 张梓浩
福建农林大学园艺植物生物工程研究所 福州 350002摘 要 茉莉酸甲酯(MeJA)
Author(s):
Effects of methyl jasmonate on the contents and relate metabolic genes of flavonoids and carotenoids in suspension cells of Amaranthus tricolor L.
LIU Shengcai#, PAN Junfei#, WANG Xiao , ZHAO Chunli, LAI Zhongxiong ** & ZHANG Zihao Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
关键词:
苋菜悬浮细胞MeJA类黄酮类胡萝卜素基因表达
Keywords:
Amaranthus tricolor L. suspension cells methyl jasmonate flavonoids carotenoids gene expression
Abstract:
Objectives: Methyl jasmonate ( MeJA) plays an important role in the regulation of plant growth and secondary metabolite synthesis. This study aimed to explore the effect of MeJA on the synthesis of flavonoids and carotenoids in amaranth cells. Methods: The effects of different concentrations of MeJA and time-added treatment on the content and yield of flavonoids and carotenoids were studied by using suspension cells of amaranth. The relationship between the expression of flavonoids and carotenoids-related genes and their accumulation was analyzed by real-time quantitative PCR.Results: The results showed that the dry weight, flavonoids and carotenoids production of amaranth suspension cells reached the maximum when with 200 μmol/L MeJA treatment. The content and yield of flavonoids and carotenoids reached the maximum when MeJA was added at 200 μmol/L on the 4th day of suspension cell culture. Under different concentrations of MeJA, the expression levels of PAL, F3H, CHI and CHS were significantly up-regulated, and were positively correlated with flavonoid content, PDS, PSY and ZDS genes were also significantly up-regulated, and were positively correlated with the carotene content . With the delay of MeJA addition time, the expression patterns of PAL, F3H, CHS and CHI genes were not significantly correlated with flavonoids content; PDS and PSY expression patterns were basically the same, and was consistent with the change of carotenoid content, and the ZDS gene is down-regulated. Conclusions: The concentration and addition time of MeJA played an important role in the regulation of flavonoids and carotenoids in suspension cells of amaranth, which laid a foundation for further study on the mechanism of MeJA-induced flavonoids and carotenoids in amaranth suspension cells.

相似文献/References:

[1]彭丽云,王云,孙雪丽,等.苋菜AmMYB2基因密码子偏好性与进化分析[J].应用与环境生物学报,2019,25(03):679.[doi:10.19675/j.cnki.1006-687x.201809020]
 PENG Liyun,WANG Yun,SUN Xueli,et al.Codon bias and evolutionary analysis of the AmMYB2 Gene in Amaranthus tricolor L.[J].Chinese Journal of Applied & Environmental Biology,2019,25(04):679.[doi:10.19675/j.cnki.1006-687x.201809020]
[2]王晓 赵春丽 彭丽云 赖钟雄** 刘生财**.苋菜蓝光受体基因AmCRY1密码子使用模式及分析*[J].应用与环境生物学报,2020,26(01):1.[doi:10.19675/j.cnki.1006-687x.2019.04056]
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更新日期/Last Update: 2019-02-01