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[1]谈高维 韦布春 崔永亮 陈香归 闫敏 沈甜 秦诗杰 羊鑫 江鑫 余秀梅**.镉钝化细菌对水稻幼苗镉吸收的影响*[J].应用与环境生物学报,2019,25(05):1-12.[doi:10.19675/j.cnki.1006-687x.2018.12006]
 TAN Gao wei,WEI Buchun,CUI Yongliang,et al.Effects of cadmium passivation bacteria on the growth and cadmium adsorption of rice seedlings*[J].Chinese Journal of Applied & Environmental Biology,2019,25(05):1-12.[doi:10.19675/j.cnki.1006-687x.2018.12006]
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镉钝化细菌对水稻幼苗镉吸收的影响*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年05期
页码:
1-12
栏目:
研究论文
出版日期:
2019-08-25

文章信息/Info

Title:
Effects of cadmium passivation bacteria on the growth and cadmium adsorption of rice seedlings*
作者:
谈高维1 韦布春1 崔永亮2 陈香归1 闫敏1 沈甜1 秦诗杰1 羊鑫1 江鑫1 余秀梅1**
1四川农业大学资源学院 成都 611130
2四川省自然资源科学研究院 成都 610041
Author(s):
TAN Gao wei1 WEI Buchun1 CUI Yongliang2 CHEN Xianggui 1 YAN Min1 SHEN Tian 1 QIN Shijie1 YANG Xin1 JIANG Xin1 & YU Xiumei1**
1. College of resources Sichuan Agricultural University, Chengdu 611130, China; 2. Natural resources research institute of Sichuan province , Chengdu 610041, China
关键词:
钝化水稻幼苗细菌促生
Keywords:
cadmium passivation rice seedling bacteria growth promoting
DOI:
10.19675/j.cnki.1006-687x.2018.12006
摘要:
根际重金属钝化微生物是否会影响作物生长及对土壤重金属吸收尚不清楚。将来源于镉污染水稻土的镉钝化细菌枯草芽孢杆菌CDR-1、雷氏普罗威登斯菌CDR-2、阿耶波多氏芽孢杆菌CDR-3分别接种到水稻幼苗根际,通过测定水稻苗的生长、生物量及镉含量来分析根际镉钝化细菌对水稻生长及镉吸收的影响。结果显示, 镉钝化细菌CDR-1、CDR-2、CDR-3的Cd2+最低抑制浓度分别为200 mg/L、200 mg/L、400 mg/L,其在20 mg/L Cd2+溶液中的镉钝化率均达到100%,且随Cd2+浓度的上升镉钝化率呈现下降趋势。在5 mg/L和10 mg/L Cd 2+胁迫下,这三株细菌对水稻幼苗的生长有不同程度的影响,并都能显著降低水稻幼苗根和地上部分的镉含量降低,其中 CDR-1对水稻幼苗的促生降镉作用最优;在0.5 mg/kg、1 mg/kg和2 mg/kg Cd2+胁迫下,CDR-1仍然能显著促进水稻幼苗生长,增加水稻幼苗生物量,并能使水稻幼苗根和地上部分的镉含量降低34.88%-61.63%。本研究表明,Cd2+钝化细菌能够显著降低水稻幼苗的镉吸收量,Cd2+钝化促生细菌CDR-1可应用于镉污染农田土壤的生物修复。
Abstract:
Whether heavy metal passivating microorganisms affect crop growth and uptake of soil heavy metals is not clear. This study focuses on the ef fects of cadmium (Cd)-resistant bacteria on the growth and Cd uptake of rice seedlings under Cd stress. The cadmium-passivating bacteria of Bacillus subtilis CDR-1, Bacillus aryabhattai CDR-2 and Providencia rettgeri CDR-3 derived from cadmium-contaminated paddy soil was respectively inoculated into the rhizosphere of rice seedlings , and the effects of cadmium passivating bacteria in the rhizosphere on the growth and cadmium absorption of rice were analyzed by measuring the growth biomass and cadmium content of rice seedlings . The Cd2+ lowest inhibition concentration of CDR-1, CDR- 2 and CDR-3 were 200 mg/L, 200 mg/L and 400 mg/L, respectively. Their Cd2+ passivation rates were 100% in the 20 mg/L of Cd 2+ solution. The Cd 2+ passivation rate decreased with the Cd2+ concentration increase. Under stress of 5 mg/L and 10 mg/L Cd 2+, the three bacteria strains have different degrees of influence on the growth of rice seedlings, and all of them can significantly reduce the cadmium content in the root and overground parts of rice seedlings , among which the role of CDR-1 in promoting growth and decreasing cadmium co ntent of rice seedlings is the best. Under the stress of 0.5 mg/kg, 1 mg/kg and 2 mg/kg Cd 2+, CDR-1 can still significantly promote rice seedlings growth, increase the biomass of rice seedlings, and reduce the content of cadmium in the root and overground parts of rice seedlings by 34.88-61.63%. This study showed that the Cd 2+ passivating bacteria can significantly reduce cadmium uptake in rice seedlings, and the Cd 2+ passivating and growth promoting bacteria CDR-1 can be applied to the bioremediation of cadmium contaminated farmland soil.

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备注/Memo

备注/Memo:
收稿日期 Received: 2018-12-04 接受日期 Accepted: 2019-03-14
*四川省科技厅重点研发项目(2017SZ0183、2017SZ0087)、国家自然科学基金(31872696)、四川省重大科技专项课题(2017NZDZX0003)、四川省科技创新苗子工程项目(2017RZ0064)、四川农业大学大学生创新训练计划项目(201710626077)资助
**通讯作者 Corresponding author (E-mail: yuxiumeicool@163.com)
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更新日期/Last Update: 2019-03-15