|本期目录/Table of Contents|

[1]廖泽东 王雪梅 卢志宇 周洋莹 代 敏 孙丰慧**.香叶醇联合柠檬醛体外抗MRSA的优化配比[J].应用与环境生物学报,2019,25(04):1-5.
 LIAO Ze-Dong,WANG Xue-Mei,LU Zhi-Yu,et al.n vitro antibacterial activity and the optimum ratio of geraniol combined with citral against MRSA[J].Chinese Journal of Applied & Environmental Biology,2019,25(04):1-5.
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香叶醇联合柠檬醛体外抗MRSA的优化配比()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年04期
页码:
1-5
栏目:
出版日期:
2019-08-31

文章信息/Info

Title:
n vitro antibacterial activity and the optimum ratio of geraniol combined with citral against MRSA
作者:
廖泽东12 王雪梅12 卢志宇12 周洋莹12 代 敏12 孙丰慧12**
1.成都医学院,四川省动物源性食品兽药残留防控技术工程实验室,成都 610500;2.成都医学院,检验医学院,成都 610500
Author(s):
LIAO Ze-Dong12 WANG Xue-Mei12 LU Zhi-Yu12 ZHOU Yang-Ying12 DAI Min12 & SUN Feng-Hui**12
1 Sichuan Provincial Engineering Laboratory for Prevention and Control Technology of Veterinary Drug Residue in Animal-origin Food, Chengdu Medical College, Chengdu, 610500, China; 2 School of Laboratory Medicine, Chengdu Medical College, Chengdu, 610500, China
关键词:
香叶醇柠檬醛耐甲氧西林金黄色葡萄球菌联合用药
Keywords:
geraniol citral MRSA drug combination
摘要:
通过体外抑菌实验,研究香叶醇与柠檬醛联合使用抗耐甲氧西林金黄色葡萄球菌(Methicillin resistant Staphylococcus aureus, MRSA)的体外抑菌活性,并采用单纯形格子混料设计优化二者的比例。微量稀释法测定香叶醇和柠檬醛对43株MRSA的最低抑菌浓度(minimum inhibitory concentration, MIC)和最低杀菌浓度(minimum bactericidal concentration , MBC);棋盘法测定香叶醇和柠檬醛联合使用时的分级抑菌浓度(Fractional inhibitory concentration FIC);单纯形格子设计优化香叶醇和柠檬醛联合的比例,并采用微量稀释法。香叶醇的MIC值为0.3~0.67 mg/mL;柠檬醛的MIC值为0.66~42.2mg/mL。二者联合使用时,以协同作用为主,占46.5%;其次为相加作用,占41.%。单纯形格子设计优化香叶醇和柠檬醛联合使用的比例为香叶醇84.5%,柠檬醛15.5%。采用此测定了香叶醇和柠檬醛混合液对43株MRSA的MIC值,发现:混合液MIC50、MIC90分别为0.25 mg/mL和0.44 mg/mL;与香叶醇单独使用相比,MIC50 和MIC90分别约为单独使用时的1/2和 2/3;与柠檬醛单独使用相比,MIC50和MIC90分别约为单独使用时的1/3和 1/4;表明二者联合使用时可起到增敏的效果,降低彼此的用量。
Abstract:
Objective: To study the in vitro antibacterial activity of geraniol combined with citral against methicillin resistant Staphylococcus aureus (MRSA), and to optimize the ratio of geraniol and citral by the simple lattice mixture design test. Methods: Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of geraniol and citral against 43 strains of MRSA were determined by microdilution method, and partial inhibitory concentration (FIC) of geraniol combined with citral was determined by chessboard method. Simplex lattice design test was used to optimize the ratio of geraniol and citral. The MIC values of the optimum mixture against the tested MRSA strains were determined. Results: The MIC values of geraniol and citral were 0.34-0.67 mg/mL and 0.66-42.23 mg/mL respectively. When the geraniol and citral combined, the synergistic effect was the main factor, accounting for 46.51%, followed by additive effect, accounting for 41.86%. Simplex lattice design model was established to optimize the ratio of geraniol and citral in combination. The optimum ratio was 84.50% geraniol and 15.50% citral. The MIC values of the optimum mixture against the tested MRSA strains were determined. It was found that the MIC50 and MIC90 of mixed solution were 0.25 mg/mL and 0.44 mg/mL, respectively. when compared to geraniol alone, the MIC50 and MIC90 were about 1/2 and 2/3 of geraniol single use; when compared to citral alone, the MIC50 and MIC90 were about 1/3 and 1/4 of citral single use.Conclusions: The results show that geraniol combined with citral exhibited better antimicrobial activity against MRSA and can significantly reduce the dosage of geraniol and citral

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备注/Memo

备注/Memo:
收稿日期 Received: 2018-11-12 接受日期 Accepted: 2019-01-29
更新日期/Last Update: 2019-02-01