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[1]吴华彰 洪 运 池宇欣 赵云利**.转录因子ATF-7参与调控GO致秀丽线虫的毒效应*[J].应用与环境生物学报,2019,25(04):1-6.[doi:Doi: 10.19675/j.cnki.1006-687x.2018.11015]
 WU Huazhang,HONG Yun,CHI Yuxin & ZHAO yunli**.Identification the transcription factor ATF-7 in the regulation of the GO toxicity in Caenorhabditis elegans*[J].Chinese Journal of Applied & Environmental Biology,2019,25(04):1-6.[doi:Doi: 10.19675/j.cnki.1006-687x.2018.11015]
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转录因子ATF-7参与调控GO致秀丽线虫的毒效应*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年04期
页码:
1-6
栏目:
研究论文
出版日期:
2019-08-31

文章信息/Info

Title:
Identification the transcription factor ATF-7 in the regulation of the GO toxicity in Caenorhabditis elegans*
文章编号:
201811015
作者:
吴华彰1 洪 运2 池宇欣2 赵云利2**
1蚌埠医学院生物科学系 蚌埠 2330302 蚌埠医学院公共卫生学院 蚌埠 233030
Author(s):
WU Huazhang1 HONG Yun2 CHI Yuxin2 & ZHAO yunli2**
1 School of Life Science, Bengbu Medical College, Bengbu, 233030, China 2 Department of Preventive Medicine, Bengbu Medical College, Bengbu, 233030, China
关键词:
转录因子秀丽线虫氧化石墨烯(GO)ATF-7
Keywords:
transcription factor C elegans graphene oxide (GO) ATF-7
DOI:
Doi: 10.19675/j.cnki.1006-687x.2018.11015
摘要:
ATF-7是具有亮氨酸拉链结构的转录激活因子,尚不清楚其是否参与氧化石墨烯(GO)所致毒效应的调控。论文以秀丽线虫为在体研究模型,以运动行为、肠道ROS水平和寿命分析,评价atf-7 RNA干扰对秀丽线虫应答GO毒效应的影响;同时以qRT-PCR和Patf-7::mCherry转基因秀丽线虫检测GO对atf-7表达水平的影响。研究结果显示,GO暴露可以降低秀丽线虫头部摆动和身体弯曲频率,且运动行为的下降与GO暴露浓度呈正相关;atf-7干扰秀丽线虫对GO所致毒效应更加敏感,0.1 mg L-1 GO就可导致头部摆动和身体弯曲频率显著下降(P<0.01);在GO暴露条件下,atf-7干扰秀丽线虫的平均寿命由13.54天缩短到8.8天,寿命缩短程度大于空载干扰组(P<0.01);atf-7干扰秀丽线虫在GO暴露条件下肠道ROS水平比未暴露情况下上升了3.33倍,而空载组肠道ROS水平上升了2.66倍,atf-7干扰秀丽线虫对GO所致肠道ROS的积累更加明显(P<0.01)。GO暴露可以诱导秀丽线虫atf-7的表达水平,GO暴露组atf-7在转录水平和蛋白水平较对照组分别上调了164倍和200倍。因此,atf-7是秀丽线虫应对GO所致毒效应中所必须的,秀丽线虫通过上调atf-7的表达以应对GO毒效应,对秀丽线虫进行atf-7干扰降低其表达水平可使秀丽线虫对GO所致毒效应更加敏感。
Abstract:
atf-7 encodes leucine zipper ( bZIP) transcription factor, but its funcion in regulating the response to graphene oxide (GO) is still largely unclear. To investigate the in vivo function of atf-7 in responsing to GO, C elegans was used as an assay system. RNA interference the in vivo expression of atf-7 were used to knockdown its expression, then locomotion behaviors, lifespan, the ROS level in the intestine and the expression at the transcriptional and protein levels were detected with or without GO exposure. The locomotion behaviors were reduced by GO in a concentration-dependent manner; the atf-7 RNAi strains showed more susceptible to GO with reduced head thrashes and body bends in the concentraion of 0.1 mg L -1 (P<0.01); the reduction level of mean lifespan was greater in the atf-7 RNAi strains (declined from 13.54 days to 8.8 days) than those of empty vector RNAi strans when treated with GO ( P<0.01); the atf-7 RNAi strains generated more ROS in the intestine after GO exposure, which was 3.33 times higher than that without exposure, while the empty vector group increased by 2.66 times ( P<0.01). Under the condition of GO exposure, the expressions of atf-7 at the transcriptional and protein levels were up-regulated by 164-fold and 200-fold, respectively. The transcription factor ATF-7 was necessary for C elegans to deal with the toxic effects caused by GO and the nemotodes were more susceptible to GO while atf-7 was knockdown by RNAi.

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备注/Memo

备注/Memo:
收稿日期:2018-11-03 接受日期 Accepted: 2019-01-03
*国家自然科学基金项目(21707002)、安徽省高校优秀青年人才支持计划重点项目(.gxyqZD2016162);蚌埠医学院自然科学基金重点项目(BYKY1603ZD)资助?
**通讯作者(E-mail: yunli201@126.com)
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更新日期/Last Update: 2019-01-24