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[1]李 娜** 彭 乾 何 健 张 浩 黄红慧 李景照 惠丰立.麦草畏降解菌株Sphingobium sp. Dca-5的分离、鉴定及降解特性*[J].应用与环境生物学报,2019,25(04):1-10.[doi:10.19675/j.cnki.1006-687x.2018.11002]
 LI Na **,PENG Qian,HE Jian,et al.Isolation, identification and the degradation characteristics of a dicamba-degrading bacterium Sphingobium sp. Dca-5*[J].Chinese Journal of Applied & Environmental Biology,2019,25(04):1-10.[doi:10.19675/j.cnki.1006-687x.2018.11002]
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麦草畏降解菌株Sphingobium sp. Dca-5的分离、鉴定及降解特性*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年04期
页码:
1-10
栏目:
研究论文
出版日期:
2019-08-31

文章信息/Info

Title:
Isolation, identification and the degradation characteristics of a dicamba-degrading bacterium Sphingobium sp. Dca-5*
文章编号:
201811002
作者:
李 娜1** 彭 乾2 何 健2 张 浩1 黄红慧1 李景照1 惠丰立1
1南阳师范学院生命科学有技术学院 南阳 473061 2南京农业大学生命科学学院 南京 210095
Author(s):
LI Na 1** PENG Qian 2 HE Jian 2 ZHANG Hao1 HUANG Honghui1 LI Jingzhao1 HUI Fengli1
1 School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, China 2 College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China
关键词:
麦草畏分离鉴定Sphingobium sp. Dca-5脱甲基酶代谢途径细胞色素P450类单加氧酶
Keywords:
dicamba isolation and identification Sphingobium sp. Dca-5 demethylase metabolic pathway cytochrome P450 monooxygenase
DOI:
10.19675/j.cnki.1006-687x.2018.11002
摘要:
麦草畏是一种广谱高效除草剂,随着抗麦草畏转基因作物的推广和使用,麦草畏的使用量急剧增加。为研究麦草畏的微生物降解机制,发掘新的麦草畏高效降解菌株和基因资源,本研究从农药厂排污口土壤中筛选到一株以麦草畏为唯一碳源生长的鞘酯菌属(Sphingobium sp.)细菌Dca-5。该菌株48 h 内完全矿化2.0 mM的麦草畏,并检测到代谢产物3,6-二氯水杨酸,表明该菌株通过脱甲基起始麦草畏降解。Dca-5粗酶液在添加NADH时具有麦草畏脱甲基酶活性,但加入细胞色素P450抑制剂1-氨基苯并三唑、增效醚或马拉硫磷后酶活受到显著抑制。在Dca-5基因组中找到两个3,6-二氯水杨酸降解基因簇但未发现DMO和dmt同源基因。以上结果表明菌株Dca-5中的麦草畏脱甲基酶是一个新的细胞色素P450类单加氧酶。因此本研究为阐明麦草畏的微生物降解代谢机制,获得新的麦草畏脱甲基酶基因提供了菌株材料和理论依据。并且Sphingobium sp. Dca-5能快速矿化麦草畏,在麦草畏污染土壤的微生物修复方面具有良好的应用前景。
Abstract:
Objectives: Dicamba is a broad-spectrum and high-efficiency herbicide, Bio giant Monsanto Biotechnology Company has successfully constructed dicamba-resistant genetically modified crops and these transgenic crops have been commercially planted on a large scale. The annual use of dicamba significantly increased. The object of this study is to isolate new dicamba-degrading strains and new dicamba-degrading gene resources.Methods and results: In this study, a dicamba-degrading bacterium Dca-5 was isolated from the sludge in the wastewater outlet of a pesticide plant and was identified as Sphingobium sp. . S train Dca-5 could completely degrade 2.0 mM dicamba within 48 h. 3,6-D ichlorosalicylate (3,6-DCSA) was identified as an intermediate, indicating that the first step of dicamba degradation was demethylation in strain Dca-5. The crude enzyme of strain Dca-5 showed dicamba demethylase activity only in the presence of NADH, and the dicamba demethylase activity was severely inhibited by addition of 1-aminobenzotriazole (ABT) , piperonyl butoxide (PBO) or malathion, which were the cytochrome P450 inhibitors. Furthermore, the previously reported dicamba demethylase genes DMO and dmt were not present in genome of strain Dca-5. Conclusions: Based on the above analyses, a dicamba-utilizing bacterium Sphingobium sp. Dca-5 was isolated, and the dicamba demethylase in the strain Dca-5 was a novel cytochrome P450-type monooxygenase that different from the previously reported demethylases. This study facilitates further elucidation of the catabolism of dicamba and provides an excellent strain source to clone dicamba-detoxification gene. Furthermore, strain Dca-5 could rapidly min eralized dicamba, thus is a good candidate for bio-remediation of dicamba-contaminated environment.? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ??

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备注/Memo

备注/Memo:
收稿日期:2018-11-02 接受日期 Accepted: 2018-11-22*国家自然科学基金项目(31570021, 31300168)资助 **通讯作者(E-mail: nali_nynu@yeah.net)点击摘要页题目后的“PDF”可下载阅读全文;本文为已录用的作者修定稿,尚未经编辑全面修改。引用本文请注明出处本刊;发表刊期和页码将以正式出版时的安排为准,但DOI确定不变。
更新日期/Last Update: 2018-12-28