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[1]路文硕 田兴军 王 强**.常见离子对漆酶降解活性艳蓝的调控作用*[J].应用与环境生物学报,2019,25(04):1-9.[doi:10.19675/j.cnki.1006-687x.2018.10031]
 LU Wenshuo,TIAN Xingjun & WANG Qiang**.Regulation effects of co mmon ions on degradation rate of reactive brilliant b lue decomposed by laccase*[J].Chinese Journal of Applied & Environmental Biology,2019,25(04):1-9.[doi:10.19675/j.cnki.1006-687x.2018.10031]
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常见离子对漆酶降解活性艳蓝的调控作用*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年04期
页码:
1-9
栏目:
研究论文
出版日期:
2019-08-31

文章信息/Info

Title:
Regulation effects of co mmon ions on degradation rate of reactive brilliant b lue decomposed by laccase*
文章编号:
201810031
作者:
路文硕 田兴军 王 强**
南京大学生命科学学院 南京 210023
Author(s):
LU Wenshuo TIAN Xingjun & WANG Qiang**
?School of Life Sciences, Nanjing University , Nanjing 210023, China
关键词:
漆酶漆酶–介体系统活性艳蓝离子降解率活性位点
Keywords:
laccase laccase-mediator system reactive brilliant blue ions degradation rates active sites
DOI:
10.19675/j.cnki.1006-687x.2018.10031
摘要:
漆酶具有很高的工业价值,但水体中离子的存在为其推广使用带来了巨大挑战。探讨了水体中常见的7种阳离子和8种阴离子调控自由漆酶和漆酶–介体系统对水中活性艳蓝降解速率的差异。研究结果显示,Mn2+, Cu2+ , Zn2+ , HPO42- 和NO2-在0-10 mmol/L范围内可促进漆酶对活性艳蓝的降解,降解速率由高到低依次为NO2-,Mn2+,Cu2+, Zn 2+, HPO 42-;SO32- , Cl-, Fe2+和Fe3+对活性艳蓝的降解有抑制作用,其中Fe2+和SO32-的最低抑制浓度分别为5 mmol/L和0.5 mmol/L;其他离子对漆酶降解活性艳蓝没有显著性影响。在5种介体的协同作用中,紫脲酸(VA)的效果最佳,导致漆酶对活性艳蓝的降解速率提升10%左右,其余介体的促进效率明显低于VA。在同等离子的调控下,漆酶–介体系统中的降解速率优于自由漆酶。这些结果初步揭示了漆酶对活性艳蓝的降解机理,同时可以通过控制水体中离子的种类和数量来更好地实现漆酶对活性艳蓝的降解,可为漆酶在实际染料废水处理中的应用提供理论依据。(图4表4参40)
Abstract:
Objectives: Laccase plays a key role in degradation of dye wastewater in industry, however a major challenge is the presence of various ions which limiting its potential application in bioremediation. Methods: This study compared the degradation rates of reactive brilliant blue regulated by seven metal ions and eight anions in free laccase system and laccase-mediator system.Results: The results showed that Mn2+, Cu2+, Zn2+, HPO42- and NO2- could promote the degradation rates of reactive brilliant blue decomposed by laccase. The degradation rates from the highest to the lowest were NO 2-, Mn2+, Cu2+, Zn2+, HPO42-. In contrast to, the additions of Cl-, Fe2+and Fe3+ at low dosage significantly inhibited degradation rates of reactive brilliant blue decomposed by laccase. The minimum inhibitory concentrations of Fe 2+ and SO 32- were 5 mmol/L and 0.5 mmol/L, respectively. Moreover, the degradation rates of reactive brilliant blue had no significant difference mediated by other icons. In addition, we selected five redox-mediating compounds which follow distinct oxidation pathways during the degradation duration of RBBR. There was the highest degradation rate of reactive brilliant blue in laccase-VA system, which was about 10% higher than in pure laccase system. The degradation rate in laccase-mediator system is better than that in free laccase system when under the control of equivalent ions. Conclusions: Therefore, For the sake of providing theoretical bases for the application of laccase in the treatment of dye wastewater, the aims of the paper is to analyze the degradation rates of reactive brilliant blue by controlling the types and concentration of ions in free laccase system and laccase-mediator system.? ??

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备注/Memo

备注/Memo:
收稿日期: 2018-10-31 接受日期 Accepted: 2019-01-26*国家自然科学基金重点项目(31530007)、国家重点研发计划项目(2016YFD0600204)和江苏省林业三新工程项目(LYSX[2016]46)资助 **通讯作者(E-mail: wangq@nju.edu.cn)点击摘要页题目后的“PDF”可下载阅读全文;本文为已录用的作者修定稿,尚未经编辑全面修改。引用本文请注明出处本刊;发表刊期和页码将以正式出版时的安排为准,但DOI确定不变。
更新日期/Last Update: 2019-02-01