|本期目录/Table of Contents|

[1]岳士忠 张慧琦 黄财德 乔玉辉**.不同基质及硒添加量对蚯蚓生长、繁殖和富硒能力的影响*[J].应用与环境生物学报,2019,25(04):1-11.[doi:10.19675/j.cnki.1006-687x.2018.10014]
 YUE Shizhong,ZHANG Huiqi,HUANG Caide,et al.Effects of different culture materials and selenium content on the growth, reproduction, and selenium-enriched ability of earthworm Eisenia fetida*[J].Chinese Journal of Applied & Environmental Biology,2019,25(04):1-11.[doi:10.19675/j.cnki.1006-687x.2018.10014]
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不同基质及硒添加量对蚯蚓生长、繁殖和富硒能力的影响*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年04期
页码:
1-11
栏目:
研究论文
出版日期:
2019-08-31

文章信息/Info

Title:
Effects of different culture materials and selenium content on the growth, reproduction, and selenium-enriched ability of earthworm Eisenia fetida*
文章编号:
201810014
作者:
岳士忠 张慧琦 黄财德 乔玉辉**
中国农业大学资源与环境学院生态科学与工程系 北京 100193
Author(s):
YUE Shizhong ZHANG Huiqi HUANG Caide QIAO Yuhui**
?College of Resources and Environment, China Agricultural University, Beijing 100193, China
关键词:
赤子爱胜蚓生物量繁殖硒含量富集系数
Keywords:
Eisenia fetida biomass reproduction selenium concentration bioaccumulation factor
DOI:
10.19675/j.cnki.1006-687x.2018.10014
摘要:
蚯蚓作为潜在的富硒蛋白饲料,在培养过程中其生长繁殖及对硒的富集受到不同培养基质及硒添加量的影响。研究在大棚环境条件下不同配比的3种农业废弃物(牛粪、菌渣和残余菜叶)和低浓度硒(Na2SeO3, 0/20/40 mg/kg)对赤子爱胜蚓(Eisenia fetida)生长繁殖和富硒能力的影响。结果发现,培养45 d 之后,与相同浓度硒处理的其他三组基质相比(纯牛粪、牛粪+菌渣+菜叶(干重比m/m/m = 6/2/2 和3/6/1)),牛粪+菌渣组(m/m = 1/1)蚯蚓总生物量、蚓茧数、蚓体硒富集量和富集系数均达到最大:其中总生物量比培养前最大增加了18.21%,每100条蚯蚓产蚓茧数量达68.35-72.45个,蚓体硒富集量最高达到151.56 mg/kg,蚓体硒富集系数最大为4.91。当硒添加浓度为20 mg/kg 和40 mg/kg时,以牛粪+菌渣+菜叶为基质的两组(m/m/m = 6/2/2, 3/6/1 )在培养初期对蚯蚓活性和培养后的总生物量增加均有一定的抑制作用,蚯蚓总生物量最大降低了8.48%。在不同基质培养条件下,外源添加硒对蚓体硒富集量和硒富集系数增加有促进作用,蚓体硒富集量与基质硒添加量均呈现出剂量—效应关系。本研究表明,赤子爱胜蚓对硒具有极强的富集能力且培养基质种类对蚯蚓的生长繁殖具有很大的影响,牛粪和菌渣组合(m/m = 1/1)是最佳的富硒蚯蚓培养基质且基质硒添加浓度≤ 40 mg Se/kg 不影响蚯蚓生长繁殖。(表4 图3 参25)
Abstract:
Objectives: To study the effects of three different agricultural wastes and selenite doses on earthworm growth, reproduction and selenium (Se) enrichment in their tissues.Methods: Earthworms were cultured with three agricultural wastes (fermented cow manure, mushroom residue, and vegetable residue) and low concentrations of selenite (0/20/40 mg/kg) for 45 days under greenhouse conditions.Results: After 45 days of cultivation, the total biomass gain, cocoon production, Se bioaccumulation and Se bioaccumulation factors ( BAFSe) in earthworm tissues were the highest when exposed to cow manure and mushroom residue (mass ration: m/m = 1/1) compared t o other three different substrates (pure cow manure, cow manure + m ushroom residue + vegetable residue, m/m/m = 6/2/2 and 3/6/1) . That is, the maximum total biomass increased by 18.21% than their initial weight , the number of cocoon reached up to 68.35-72.45 per 100 earthworm individuals, Se bioaccumulation concentration in earthworm tissues was high as 151.56 mg/kg dry weight, the maximum value of Se BAFSe was 4.91 in the substrate of cow manure and mushroom residue. When Se addition contents in the substrates based on cow manure + m ushroom residue + vegetable residue ( m/m/m = 6/2/2 and 3/6/1) w ere 20 and 40 mg/kg, the activit ies of earthworms in the early stage of cult ivation were inhibited, as well as the increase of total biomass after cultivation , and the total earthworms biomass decreased by 8.48%. Se bioaccumulation amounts in earthworm tissues were significantly affected by selenite doses in the four different substrates, showing a dose-dependent relationship.Conclusions: The substrate types have a great influence on the growth and reproduction of earthworm; E. fetida had strong enrichment ability for selenite; cow manure and mushroom residue was the best treatment and Se addition with less than 40 mg Se/kg did not affect the growth and reproduction of earthworms in this study.

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备注/Memo

备注/Memo:
收稿日期:2018-10-17 接受日期 Accepted: 2019-01-25*公益性行业(农业)科研专项(201303106)和生物多样性与有机农业北京市重点实验室项目(XK10019440)资助 **通讯作者(E-mail:qiaoyh@cau.edu.cn)点击摘要页题目后的“PDF”可下载阅读全文;本文为已录用的作者修定稿,尚未经编辑全面修改。引用本文请注明出处本刊;发表刊期和页码将以正式出版时的安排为准,但DOI确定不变。
更新日期/Last Update: 2019-02-01