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[1]高桂青 简敏菲 卢 龙** 计 勇 王香莲 王 岩 王雪茹.Cu2+、Cd2+胁迫对马来眼子菜光合色素及光合荧光特性的影响*[J].应用与环境生物学报,2019,25(03):1-9.[doi:10.19675/j.cnki.1006-687x.2018.10003]
 GAO Guiqing,JIAN Minfei,LU Long**,et al.Effects of Cu2+ or Cd2+ Stress on Photosynthetic Pigment and Photosynthetic Fluorescence Characteristics of Potamogeton Malaianus*[J].Chinese Journal of Applied & Environmental Biology,2019,25(03):1-9.[doi:10.19675/j.cnki.1006-687x.2018.10003]
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Cu2+、Cd2+胁迫对马来眼子菜光合色素及光合荧光特性的影响*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年03期
页码:
1-9
栏目:
研究论文
出版日期:
2019-06-25

文章信息/Info

Title:
Effects of Cu2+ or Cd2+ Stress on Photosynthetic Pigment and Photosynthetic Fluorescence Characteristics of Potamogeton Malaianus*
文章编号:
201810003
作者:
高桂青12 简敏菲3 卢 龙1** 计 勇2 王香莲2 王 岩2 王雪茹2
1南昌大学资源环境与化工学院 南昌 330031 2南昌工程学院土木与建筑工程学院 南昌 330099 3江西师范大学生命科学学院 南昌 330022
Author(s):
GAO Guiqing12 JIAN Minfei3 LU Long1** JI Yong2WANG Xianglian2WANG Yan2 & WANG Xueru2
?1School of Resource Environment and Chemical Engineering, Nanchang University, Nanchang 330031, China 2School of Civil and Architecture Engineering, Nanchang Institute of Technology, Nanchang 330099, China 3College of Lifte Sciences,Jiangxi Normal University , Nanchang 330022, China
关键词:
马来眼子菜重金属胁迫光合色素光合荧光荧光成像
Keywords:
Potamogeton Malaianus heavy metal stress photosynthetic pigment photosynthetic fluorescence fluorescence imaging
DOI:
10.19675/j.cnki.1006-687x.2018.10003
摘要:
为全面了解沉水植物受重金属胁迫后光合色素及荧光特性的变化,以马来眼子菜(Potamogeton Malaianus)为供试材料,采用不同浓度Cu2+或Cd2+处理,采用丙酮萃取和水下荧光仪原位测定法,研究重金属对植物光合色素含量、光合荧光参数及荧光成像的影响。结果表明:Cu2+或Cd2+处理下,叶绿素总量(Ct)、叶绿素a(Ca)、叶绿素b(Cb)、类胡萝卜素(Cc)均极显著下降(P<0.01),且Cu2+对叶绿体的毒害作用比Cd2+更大;最大光化学效率Fv/Fm、潜在光化学效率Fv/Fo、有效量子产量Y(Ⅱ)和光化学淬灭系数qP也呈极显著下降趋势(P<0.01),且Cu2+胁迫的影响更显著。Cu2+处理下,调节性能量耗散的量子产量Y(NPQ)和非光化学淬灭系数qN均先增加后降低。而Cd2+处理下,Y(NPQ)和qN均呈极显著上升趋势(P<0.01);各处理组非调节性能量耗散的量子产量Y(NO)差异不显著。Cu2+处理下相对电子传递速率ETR下降更多。0.5 mg L-1 Cu2+处理下,马来眼子菜光合色素含量、Fv/Fm、Fv/Fo、Y(Ⅱ) 和ETR均比对照组下降显著;0.5mg L-1和1.0 mg L-1 Cd2+处理下,马来眼子菜具有正常的光合活性。荧光成像表明叶片受损始于叶边缘,叶脉抗重金属胁迫能力强于叶肉;相同浓度处理下,Cu2+处理对叶片的损伤程度大于Cd2+处理。综上表明,马来眼子菜耐Cd2+ 能力强于Cu2+,可推测将马来眼子菜用作低浓度Cd2+污染水域的修复物种。(图5表3参39)
Abstract:
For a comprehensive of the effects of different heavy metals on plant photosynthetic pigments and photosynthetic fluorescence characteristics, Potamogeton Malaianus was selected as experimental material. Six separate concentration levels of Cu2+ or Cd 2+ were stressed on P. Malaianus. Photosynthetic pigments were determined by acetone extraction. Fluorescence parameters were detected by underwater fluorometer in situ. The total chlorophyll (Ct), chlorophyll a (Ca), chlorophyll b (Cb) and carotenoid (Cc) decreased significantly (P < 0.01) with the increase of Cu2+ or Cd 2+ concentrations. The toxic effect of Cu2+ stress on chloroplasts is greater than that of Cd 2+. Moreover, The maximum photochemical efficiency Fv/Fm, potential photochemical efficiency Fv/Fo, effective quantum yield Y(II) and photochemical quenching coefficient qP also showed a significant downward trend (P < 0.01) , and the effect of Cu2+ stress was more significant. Under Cu 2+ treatment, regulated energy dissipation quantum yield Y(NPQ) and non-photochemical quenching coefficient qN increased firstly and then decreased. Under Cd 2+ treatment, both Y(NPQ) and qN showed a very significant upward trend (P < 0.01). The difference of non-regulated energy dissipation quantum yield Y(NO) in each treatment group was not significant. The relative electron transfer rate ETR decreased more under Cu 2+ treatment. Under the treatment of 0.5 mg L -1 Cu2+, the photosynthesis pigment content, Fv/Fm, Fv/Fo, Y(II) and ETR of P.Malaianus was significantly lower than that of the control group. Under the treatment of 0.5 mg L-1 and 1.0 mg L-1 Cd2+, P.Malaianus has normal photosynthetic activity. Fluorescence imaging showed that the damage began at the edge of the leaves, and the resistance of the veins to heavy metals was stronger than that of the mesophyll. Under the same concentration treatment, the damage degree of Cu2+ treatment on the leaves was greater than that of Cd2+ treatment. P. Malaianus is more resistant to Cd2+ than Cu2+. It can be speculated that P. Malaianus is used as a repairing species in low concentration Cd 2+ contaminated waters.

相似文献/References:

[1]陈杰,许长征,曹颖倩,等.不同重金属对拟南芥根系特征的影响比较[J].应用与环境生物学报,2017,23(06):1122.[doi:10.3724/SP.J.1145.2017.03003]
 CHEN Jie,XU Changzheng,CAO Yingqian,et al.Heavy-metal-induced morphological changes of root characteristics in Arabidopsis thaliana[J].Chinese Journal of Applied & Environmental Biology,2017,23(03):1122.[doi:10.3724/SP.J.1145.2017.03003]

备注/Memo

备注/Memo:
收稿日期:2018-10-06 接受日期 Accepted: 2018-11-09 *国家自然科学基金项目(51579127,51469017)、江西省科技厅青年基金(20161BAB216109)、江西省教育厅科研技术研究项目(GJJ170978)和2016年国家大学生创新创业训练计划资助项目(201611319011)资助 **通讯作者(E-mail:lulong@ncu.edu.cn)点击摘要页题目后的“PDF”可下载阅读全文;本文为已录用的作者修定稿,尚未经编辑全面修改。引用本文请注明出处本刊;发表刊期和页码将以正式出版时的安排为准,但DOI确定不变。
更新日期/Last Update: 2018-12-25