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[1]郭 莹,王一明** 巫 攀 彭 双.长期施用粪肥对水稻土中微生物群落功能多样性的影响*[J].应用与环境生物学报,2019,25(03):1-14.[doi:10.19675/j.cnki.1006-687x.2018.09028]
 GUO Ying,WANG Yiming**,WU Pan& PENG Shuang.Influence of long-term manure application in paddy soil on functional diversity of microbial community[J].Chinese Journal of Applied & Environmental Biology,2019,25(03):1-14.[doi:10.19675/j.cnki.1006-687x.2018.09028]
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长期施用粪肥对水稻土中微生物群落功能多样性的影响*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年03期
页码:
1-14
栏目:
研究论文
出版日期:
2019-06-25

文章信息/Info

Title:
Influence of long-term manure application in paddy soil on functional diversity of microbial community
文章编号:
201809028
作者:
郭 莹1 2 王一明1** 巫 攀1 彭 双1
1中国科学院南京土壤研究所土壤与农业可持续发展国家重点实验室 南京 210008 2中国科学院大学 北京 100049
Author(s):
GUO Ying12WANG Yiming1**WU Pan1& PENG Shuang 1
1 State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China 2 University of Chinese Academy of Sciences, Beijing 100049, China
关键词:
Biolog土壤微生物粪肥施用长期定位碳素分析
Keywords:
Biolog edaphon manure application long-term fertilization carbon analysis
DOI:
10.19675/j.cnki.1006-687x.2018.09028
摘要:
为明确长期施用粪肥对农田土壤碳转化周转的影响,以中国科学院常熟农业生态实验站粪肥长期定位试验田为对象,采用Biolog技术对长期施用高量(9.0 t/ha)或低量(4.5 t/ha)粪肥(新鲜猪粪或发酵猪粪)的水稻土(0-40cm)土壤微生物群落多样性及碳源利用情况的影响进行了研究。结果显示,耕层(0-20cm)土壤所有施肥处理的微生物碳源利用率、Shannon、Simpson和McIntosh指数均显著高于无肥对照处理,且施肥处理的微生物活性与土壤全氮、有机质含量显著相关。因子分析表明,不同施肥处理碳源利用类型存在差异,主要由主成分I进行解释,方差贡献率为71.31%;醣类和双亲化合物是导致施用粪肥处理与无肥对照间产生分异的主导碳源。主成分分析发现醣类和羧酸是造成不同土层间样本点差异显著(P<0.01)的主导碳源。同时,新鲜粪肥和发酵粪肥处理土壤微生物碳源利用类型的分异主要在5~10 cm土层,低量处理组主要差异碳源为聚合物和醣类、高量粪肥处理组则主要是氨基酸和氨基化合物。研究表明,虽然不同种类的粪肥及施肥量间存在差异,但长期施用粪肥有助于提高土壤微生物的碳源利用能力和群落多样性,增强土壤有机质的转化周转能力;因此,如何合理施用粪肥等有机肥对于土壤有机质提升、耕地保育、畜禽粪便资源化都具有重要意义。图7表6参32
Abstract:
Objectives: To make the influence of long-term manure application on carbon turnover clear, and provide references for rational manure utilization as well as improvement of soil fertility.Methods: Diversity of microbial community and carbon source utilization in paddy soil (0-40cm) were studied, tested by Biolog, after long-term fertilization under different treatments (with low/high level of fresh/composted manure application) in Changshu agro-ecological experimental station, which belongs to Chinese academy of sciences. Results: Statistic analysis shows that both the carbon source utilization by microbial and diversity indexes (Shannon, Simpson and McIntosh) in fertilization treatments are significantly higher than those with CK treatment in 0-20cm plough layer, while there are no differences in 20-40cm layer. The microbial activity of fertilized treatments was significantly correlated with the content of soil organic matter and total nitrogen. Factor analysis indicates that different fertilization treatments can be recognized by various carbon utilization patterns, which are mainly explained by principal component I, and the variance contribution rate is 71.31%. Carbohydrates and amphiphilic are the dominant carbon sources that lead to differentiation between applying manure and non-fertilizer treatments, which are mainly acted by ?-Methyl-D- Glucoside, N-Acetyl-D-Glucosamine, a-D-Lactose and Pyruvic Acid Methyl Ester. Principal component analysis shows that soil samples of various depth can be differentiated by principle component I explaining the alteration of carbon source utilization (with variance contribution rate of 71.67%) where the difference among points in each soil layer reached significant level (P < 0.01). Carbohydrates and carboxylic acid are the dominant carbon sources contributing to PCAI, which are mainly acted by ?-Methyl-D-Glucoside, N-Acetyl-D-Glucosamine, a-D-Lactose, and D-Xylose.At the same time, the differences in carbon source utilization types caused by fresh and fermented manure are mainly reflected in 5-10 cm soil layer. In the group of low quantity manure application, composted manure treatments have higher utilization rate on polymer and carbohydrate than the fresh ones, while in high amount fertilization group the utilization rates of amino acids and amides are higher in composted ones than that of fresh manure application with a more significant difference. Conclusions:Although there were differences between fresh and fermented pig manure as well as different application amount, the long-term application of fresh and fermented pig dung is helpful to improve the carbon source utilization capacity and community diversity of soil microorganisms, thus enhance the conversion and turnover capacity of soil organic matter.Therefore, the rational application of organic fertilizers, such as manure, is of great significance to the improvement of soil organic matter, the conservation of farmland and the reclamation of livestock manure.? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?

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备注/Memo

备注/Memo:
收稿日期: 2018-09-20 接受日期 Accepted: 2018-11-09*中国科学院科技服务网络计划(STS计划)区域重点项目资助、宁夏回族自治区重点研发计划项目(2017BN05)资助 **通讯作者(E-mail:ymwang@issas.ac.cn)点击摘要页题目后的“PDF”可下载阅读全文;本文为已录用的作者修定稿,尚未经编辑全面修改。引用本文请注明出处本刊;发表刊期和页码将以正式出版时的安排为准,但DOI确定不变。
更新日期/Last Update: 2018-12-28