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[1]任亚峰 韦唯 李冬雪 王勇 谈孝凤 陈卓**.葡萄灰霉病病原菌鉴定及生物学特性*[J].应用与环境生物学报,2019,25(05):1-10.[doi:10.19675/j.cnki.1006-687x.2018.09026]
 REN Yafeng,WEI Wei,LI Dongxue,et al.Identification and biological characteristics of grape gray mold pathogen[J].Chinese Journal of Applied & Environmental Biology,2019,25(05):1-10.[doi:10.19675/j.cnki.1006-687x.2018.09026]
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葡萄灰霉病病原菌鉴定及生物学特性*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年05期
页码:
1-10
栏目:
研究论文
出版日期:
2019-08-25

文章信息/Info

Title:
Identification and biological characteristics of grape gray mold pathogen
作者:
任亚峰1 韦唯12 李冬雪1 王勇2 谈孝凤3 陈卓1**
1贵州大学绿色农药与农业生物工程教育部重点实验室 贵阳 550025
2贵州大学农学院 贵阳 550025
3贵州省植保植检站 贵阳 550001
Author(s):
REN Yafeng1 WEI Wei1 2 LI Dongxue1 WANG Yong2 TAN Xiaofeng3 CHEN Zhuo 1
1 Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, China
2 Agricultural College of Guizhou University, Guiyang 550025, China
3 Plant Protection Station of Guizhou Province, Guiyang 550001, China
关键词:
灰葡萄孢鉴定致病性系统发育分析生物学特性
Keywords:
Botrytis cinerea identification pathogenicity phylogenetic analysis biological characteristics
DOI:
10.19675/j.cnki.1006-687x.2018.09026
摘要:
为有效控制贵州省福泉市葡萄灰霉病的危害,对该地区病害的病原菌进行了鉴定和生物学特性研究,以便为该病害的流行机制和防治研究提供理论基础。从葡萄灰霉病样品中分离纯化了多个病原菌菌株,并进行了形态特征观察和多基因系统发育树分析。选择代表性菌株GZFQ-1进行葡萄的茎、叶片和果实的致病性分析。同时,对GZFQ-1菌株在不同温度、pH条件下的生长情况,及对不同碳源和氮源的利用进行研究。根据病原菌的形态学和分子特征,将贵州省福泉市葡萄灰霉病菌鉴定为灰葡萄孢菌(Botrytis cinerea)。该菌株在PDA培养基上生长的最适温度是25 ℃,其次为22 ℃;在pH值为5-9的范围内,该菌株均可生长,且在pH值5-8范围内,生长相对较快;以葡萄糖为碳源,以硝酸钾、异亮氨酸、组氨酸和蛋白胨为氮源时,利于菌丝发育,同时,碳、氮源也会影响菌株的生长速率和色素形成。综上所述,发生在贵州省福泉市葡萄灰霉病的病原菌为B. cinerea,且能够引起嫩茎、叶和果实发病,这一结论可为该地区葡萄灰霉病的防治提供理论指导。
Abstract:
To effectively control the damage of grape gray mold in Fuquan City, Guizhou Province, this paper identified the pathogen and studied it’s the biological characteristics in order to provide the theoretical basis for the epidemic mechanism of the disease and its control. Many strains were isolated from the samples of the disease, then the morphology of the pathogen was observed and phylogenetic analysis was conducted. Pathogenicity tests were conducted on the stems, leaves and fruits of grape plant with the representative strain GZFQ-1. The strain GZFQ-1 was observed under different temperature and different pH on PDA, as well as the utilization of nitrogen or carbon sources. Based on the characteristics of the morphology and molecular biology, the strain GZFQ-1 was identified as B. cinerea MUCL87. Its optimum temperature was 25 ℃, next was 22 ℃; The strain could grow in pH 5-9, and grow faster in pH 5-8; The carbon source of glucose, and nitrogen sources of potassium nitrate, isoleucine, histidine and peptone were optimum for strain GZFQ-1, meanwhile, different carbon and nitrogen sources could influence the growth of mycelium and formation of pigment. In conclusion, the pathogen of grape gray mold in Fuquan City, Guizhou Province was B. cinerea, and it could damage the tender stems, leaves and fruits, this result could provide theoretical guidance for the prevention and treatment of grape gray mold in this area.

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备注/Memo

备注/Memo:
收稿日期 Received: 2018-09-19 接受日期 Accepted: 2019-03-07
*国家科技支撑计划项目(2014BAD23B03)、教育部新世纪优秀人才项目(NCET-13-0748)和贵州省科技厅-黔南州人民政府农业科技合作专项计划(2013-01)资助??
通讯作者 Corresponding author (E-mail: gychenzhuo@aliyun.com)
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更新日期/Last Update: 2019-03-13