|本期目录/Table of Contents|

[1]任海伟 冯银萍 刘 通 王 昱 李志忠* 裴佳雯 王 蕾 李盼盼.温度对干玉米秸秆与废弃白菜混贮发酵品质的影响和微生物菌群解析[J].应用与环境生物学报,2019,25(03):1-14.[doi:10.19675/j.cnki.1006-687x.2018.09024]
 Ren Haiwei,Feng Yingping,Liu Tong,et al.Effects of temperature o n the mixed storage fermentation quality of dry corn stalk and cabbage waste and its microbial community analysis[J].Chinese Journal of Applied & Environmental Biology,2019,25(03):1-14.[doi:10.19675/j.cnki.1006-687x.2018.09024]
点击复制

温度对干玉米秸秆与废弃白菜混贮发酵品质的影响和微生物菌群解析()
分享到:

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年03期
页码:
1-14
栏目:
研究论文
出版日期:
2019-06-25

文章信息/Info

Title:
Effects of temperature o n the mixed storage fermentation quality of dry corn stalk and cabbage waste and its microbial community analysis
文章编号:
201809024
作者:
任海伟123 冯银萍1 刘 通1 王 昱23 李志忠1* 裴佳雯1 王 蕾1 李盼盼1
1兰州理工大学生命科学与工程学院 兰州 7300502兰州理工大学西部能源与环境研究中心 兰州 7300503甘肃省生物质能与太阳能互补供能系统重点实验室 兰州 730050
Author(s):
Ren Haiwei123 Feng Yingping1 Liu Tong1 WangYu23 Li ZhiZhong 1* Pei Jiawen1 Wang Lei 1 Li Panpan1?
1 School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China 2 China Western Energy & Environment Research Center, Lanzhou University of Technology , Lanzhou 730050, China 3 Key Laboratory of Complementary Energy System of Biomass and Solar Energy, Lanzhou 730050, China
关键词:
干玉米秸秆废弃白菜温度混贮品质微生物菌群高通量测序
Keywords:
dry corn stalk (DCS) cabbage waste (CW) storage temperature mixed silage quality microbial community diversity high-throughput sequencing
DOI:
10.19675/j.cnki.1006-687x.2018.09024
摘要:
本文旨在研究干玉米秸秆和废弃白菜在不同季节温度条件下的混合贮存品质差异性。参考西北地区气候条件设置低温(-3±1℃,LX)、室温(18±1℃,RX)和中温(34±1℃,MX)3个处理组,连续混贮90 d,间隔一定时间分析感官质量、有机组分和发酵品质,并利用Illumina Miseq高通量测序技术考察混贮发酵期间微生物菌群的动态变化。结果表明:混贮30 d时,RX和MX组中干物质和乳酸含量显著高于LX组(P<0.05),氨态氮/总氮和可溶性碳水化合物、纤维素含量显著低于LX组(P<0.05)。贮存90 d时,RX组的中性洗涤纤维含量、pH值及氨态氮/总氮均显著低于LX和MX组(P<0.05)。贮存三个月期间,RX组的乳酸/乙酸和乳酸/总有机酸比值始终高于LX组和MX组,乳酸发酵强度较高;3个处理组的生物降解潜力均高于原料,丁酸含量很低,感官质量均为优等,费氏评分等级为好或很好,均达到优良贮存品质。微生物群落结果显示,3个处理组中门水平优势菌为变形菌(Proteobacteria)和厚壁菌(Firmicutes),且LX和RX组中的厚壁菌门相对丰度较原料明显升高;属水平优势菌为肠杆菌(Enterobacteriaceae)、乳杆菌(Lactobacillus)、肉食杆菌(Carnobacterium)及明串珠菌(Leuconostoc),且LX和RX组中的肠杆菌等腐败菌相对丰度低于MX组,乳杆菌、明串珠菌和肉食杆菌等乳酸细菌的相对丰度高于MX组。总之,在室温(18±1 ℃)条件下混贮更有利于提高乳酸发酵强度,抑制腐败菌生长,从而使有机酸和有机组分得到优化重组,实现较高贮存品质。(图5表9参36)
Abstract:
The objective of this study was to investigate the difference of mixed storage quality for dry corn stalk and cabbage waste at different temperatures. Three treatments, such as low temperature (-3±1℃, LX), room temperature (18±1℃, RX) and medium temperature (34±1℃, MX), were conducted according to the climatic conditions in Northwest China. All the experimental groups were stored successively for 90 d by the theory of ensiling. Furthermore, the sensory quality, the contents of organic composition and fermentation quality were analyzed at 30 d and 90 d, respectively. Moreover, the high-throughput sequencing technology of Illumina Miseq was used to analyze the dynamic changes of bacterial community diversity during ensiling. The results showed that the contents of dry matter and lactic acid in RX and MX groups were significantly higher than that of LX group (P<0.05), the ratio of ammonia nitrogen to total nitrogen, the contents of water soluble carbohydrate and cellulose were significantly lower than that of LX group (P<0.05) in 30 d. After storage for 90 d, the content of neutral detergent fiber, pH value and the ratio of ammonia nitrogen to total nitrogen in RX group were significantly lower than that of LX and MX groups ( P<0.05). The ratio of lactic acid to acetic acid and the ratio of lactic acid to total organic acid of RX group were always higher than that of LX group and MX group throughout the storage period for three-month, indicating that the fermentation intensity of Lactobacillus series increased; the biodegradation potential was higher than that of un-stored raw materials, the content of butyric acid was lower, and the sensory quality and the Flieg score of the three treatments were all excellent that all of them reached good storage quality. The results of bacterial community diversity showed that the dominant bacteria in phylum level in the three treatments were Proteobacteria and Firmicutes, and the relative abundance of Firmicutes in LX and RX groups was significantly higher than that of raw materials. The dominant bacteria in genus level in the three treatments were Enterobacteriaceae, Lactobacillus, Carnobacterium and Leuconostoc. The relative abundance of spoilage bacteria such as Enterobacteriaceae in LX and RX group was lower than that in MX group, while the relative abundance of Lactobacillus, Leuconostoc and Carnobacterium was higher than that of MX group. In conclusion, the room temperature (18±1 ℃) was more beneficial to improve the strength of lactic acid fermentation for inhibiting the growth of spoilage bacteria, and the compositional model of organic acids and organic components were optimized and recombined to achieve higher storage quality.? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?

相似文献/References:

[1]任海伟,孙安琪,马延琴,等.添加乙酸对干玉米秸秆与白菜废弃物混贮品质的影响[J].应用与环境生物学报,2018,24(05):1087.[doi:10.19675/j.cnki.1006-687x.2017.11016]
 REN Haiwei,SUN Anqi,et al.Effects of different doses of acetic acid on mixed storage quality of dry corn stalk and Chinese cabbage waste[J].Chinese Journal of Applied & Environmental Biology,2018,24(03):1087.[doi:10.19675/j.cnki.1006-687x.2017.11016]

备注/Memo

备注/Memo:
收稿日期: 2018-09-17 接受日期 Accepted: 2018-10-15*国家自然科学基金项目(51666010,51366009)、中国博士后科学基金面上项目(2018M631217)、甘肃省自然科学基金项目(1606RJZA206, 1606RJYA287, 1606RJZA196, 17JR5RA117)和兰州市科技计划项目(2014-2-20)资助 **通讯作者(E-mail:zzli2004@lut.cn)点击摘要页题目后的“PDF”可下载阅读全文;本文为已录用的作者修定稿,尚未经编辑全面修改。引用本文请注明出处本刊;发表刊期和页码将以正式出版时的安排为准,但DOI确定不变。
更新日期/Last Update: 2018-11-19