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[1]池 婷 赵震乾 张后虎** 孔德洋.医疗废物堆置场地土壤抗生素抗性基因组成特征——以华东丘陵地区某医废堆场为例*[J].应用与环境生物学报,2019,25(03):1-13.[doi:10.19675/j.cnki.1006-687x.2018.08030]
 CHI Ting,ZHAO Zhenqian,ZHANG Houhu,et al.Characteristics of the antibiotic resistance genes in soil of medical waste disposal site?--A case study of a medical waste dispos al site in the hilly area of East China[J].Chinese Journal of Applied & Environmental Biology,2019,25(03):1-13.[doi:10.19675/j.cnki.1006-687x.2018.08030]
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医疗废物堆置场地土壤抗生素抗性基因组成特征——以华东丘陵地区某医废堆场为例*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年03期
页码:
1-13
栏目:
研究论文
出版日期:
2019-06-25

文章信息/Info

Title:
Characteristics of the antibiotic resistance genes in soil of medical waste disposal site?--A case study of a medical waste dispos al site in the hilly area of East China
文章编号:
201808030
作者:
池 婷 赵震乾 张后虎** 孔德洋
环境保护部南京环境科学研究所 南京 210042
Author(s):
CHI Ting ZHAO Zhenqian ZHANG Houhu KONG Deyang
?Nanjing Institute of Environmental Sciences (NIES), Ministry of Ecology and Environment (MEE), Nanjing, 210042, China
关键词:
抗生素抗性基因土壤医疗废物高通量测序环境污染
Keywords:
antibiotic resistance genes soil medical waste high-throughput sequencing environmental pollution
DOI:
10.19675/j.cnki.1006-687x.2018.08030
摘要:
抗生素抗性基因(Antibiotic resistance genes, ARGs)目前已经被认为是一种重要的环境污染物。土壤中存在的微生物代表了ARGs的古老进化起源之一,并且已被认为是临床病原体获得ARGs的重要交换介质。为了测试医疗废物堆置区域的ARGs丰度,工作人员在医废堆置区内外采集了共28个土壤样品。在提取了样品中的微生物宏基因组之后,对所提取的DNA进行高通量测序。将所得序列与抗生素抗性基因数据库进行比对,并通过计算不同类型以及不同亚型的ARGs 拷贝数/16S rRNA 基因拷贝数评估ARGs的组成。结果显示,医废堆置区内样本所含的氨基糖苷类、氯霉素类、磺胺类、四环素类ARGs丰度显著高于堆置区外样本(分别达到了0.0134±0.0138、0.00615±0.00747、0.0188±0.0254以及0.00504±0.00292 ARGs 拷贝数/16S rRNA 基因拷贝数),特别是sul1(磺胺类)、floR(氯霉素类)、catB(氯霉素类)、aph(3’’)-I(氨基糖苷类)和tetG(四环素类)这五类ARGs亚型。同时,医废堆置区内样本有着更高的ARGs多样性(t-test, p < 0.05)。本研究表明医疗废物堆置会显著提高区域内土壤中ARGs的丰度以及多样性从而增加潜在环境风险。
Abstract:
Antibiotic resistance genes (ARGs) have been considered as global emerging pollutants in many environmental systems. Microorganism resenting in soil represent one of the ancient evolutionary origins of ARGs and have been proposed as a reservoir for ARGs exchanging with clinical pathogens. To test the ARG profiles in the target medical wastes dumping area, 28 different soil samples were taken from the area stacked by medical waste and the nearby area. Metagenomes of the microorganisms from the soil sample were extracted for high-throughput sequencing. ARG abundances of these samples were then obtained by searching the metagenomic sequences against the antibiotic resistance genes database and evaluated by ARGs per copy of 16S rRNA gene in type level and subtype level. Overall, compare to the nearby samples, the samples from the area stacked by medical wastes significant enriched the ARGs encoding resistances to aminoglycoside, chloramphenicol, sulfonamide, and tetracycline (a chieved 0.0134±0.0138, 0.00615±0.00747, 0.0188±0.0254, and 0.00504±0.00292 ARGs copy number/16S rRNA gene copy number respectively), especially the ARGs subtypes of sul1 (sulfonamide), floR ( chloramphenicol), catB ( chloramphenicol), aph(3’’)-I (aminoglycoside) and tetG (tetracycline). Moreover, the diversity of ARGs of the samples from the polluted area was also significantly higher than it in other samples (t-test, p < 0.05). The comparatively high abundance and diversity of ARGs indicating the potential health risk of the medical wastes to the soil in the area.? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ??

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备注/Memo

备注/Memo:
收稿日期: 2018-08-31 接受日期 Accepted: 2018-11-09*中央级公益性科研院所基本业务专项项目(No. GYZX180109)和江苏省环保科研课题(2017024)资助 **通讯作者(E-mail: zhh@nies.org)点击摘要页题目后的“PDF”可下载阅读全文;本文为已录用的作者修定稿,尚未经编辑全面修改。引用本文请注明出处本刊;发表刊期和页码将以正式出版时的安排为准,但DOI确定不变。
更新日期/Last Update: 2018-12-28