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[1]徐少慧 蒋代华** 史鼎鼎 张蓉蓉 黄智刚 杨钙仁.重金属复合污染土壤中耐铅镉微生物的筛选及鉴定*[J].应用与环境生物学报,2019,25(03):1-13.[doi:10.19675/j.cnki.1006-687x.2018.08028]
 XU Shaohui,JIANG Daihua**,SHI Dingding & ZHANG Rongrong.Screening and identification of lead and cadmium resistant microorganisms from heavy metal combined pollution soil *[J].Chinese Journal of Applied & Environmental Biology,2019,25(03):1-13.[doi:10.19675/j.cnki.1006-687x.2018.08028]
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重金属复合污染土壤中耐铅镉微生物的筛选及鉴定*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年03期
页码:
1-13
栏目:
研究论文
出版日期:
2019-06-25

文章信息/Info

Title:
Screening and identification of lead and cadmium resistant microorganisms from heavy metal combined pollution soil *
文章编号:
201808028
作者:
徐少慧1 蒋代华12** 史鼎鼎1 张蓉蓉1 黄智刚12 杨钙仁3
1广西大学农学院 南宁 530004 2广西农业环境与农产品安全重点实验室培养基地 南宁 530004 3 广西大学林学院 南宁 530004
Author(s):
XU Shaohui1 JIANG Daihua12** SHI Dingding1 & ZHANG Rongrong1
1Agricultural College, Guangxi University, Nanning 530004, China 2Guangxi Key Laboratory of Agricultural Environment and Agricultural Product Safety Cultivation Base, Nanning 530004, China
关键词:
重金属污染耐铅镉微生物筛选鉴定
Keywords:
heavy metal pollution lead cadmium resistant screening identification
DOI:
10.19675/j.cnki.1006-687x.2018.08028
摘要:
为获得高耐性复合重金属微生物资源菌株,应用于微生物或微生物联合植物对重金属污染土壤的修复。采集广西桂西北尾矿区重金属污染的土壤样品,采用改良的马丁氏培养基分离和筛选既耐重金属铅又耐镉的高耐性微生物。结果获得了一株耐铅浓度为1200 mg/L、镉浓度为120 mg/L的铅镉复合耐性真菌——菌株K-3。该菌株(K-3)最适生长环境为温度30 ℃、pH值为7.0、盐浓度为3.5%。通过形态结合分子生物学鉴定得知,K-3菌株与已知分类地位的标准菌株Uncultured Westerdykella的18S rDNA 序列一致性为99%。查阅相关文献,综合推知,本文获得的铅镉复合耐性真菌——菌株K-3是发现的既耐重金属铅又耐镉的最强双耐真菌之一,为微生物或微生物联合植物修复重金属污染土壤提供了珍贵的微生物种质资源。
Abstract:
In order to obtain the microbial resource strain high compound heavy metal tolerance to remedy heavy mental contaminated soil, collected soil samples from the tail mining area in Northwest Guangxi, improved Martin’s medium was used to isolate and screen the high-tolerance microorganism for heavy metal lead and cadmium. The fungus with compound tolerance of lead and cadmium, termed as K-3 was gained, whose lesd resistance was 1200 mg/L and cadmium resistance was 120 mg/L. The optimum temperatura for K-3 was 30 ℃, whereas pH was 7.0, and salt concentration was 3.5%. The identification of morphology and molecular biology showed that the sequence consistency of 18S rDNA between K-3 and the tandard strain Uncultured Westerdykella was 99%. By consulting relevant literature and comprehensive inference, it was shown that K-3 was one of the strongest double-tolerance fungus for heavy metal lead and cadmium. It provided precious microbial germplasm resources for remedi ation of heavy metal contaminated soil using microorganism or microorganism associated plants.? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?

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备注/Memo

备注/Memo:
收稿日期: 2018-08-29 接受日期Accepted: 2018-10-17*国家自然科学基金项目(41661076)资助 **通讯作者(E-mail:dhjiang2008@gxu.edu.cn)点击摘要页题目后的“PDF”可下载阅读全文;本文为已录用的作者修定稿,尚未经编辑全面修改。引用本文请注明出处本刊;发表刊期和页码将以正式出版时的安排为准,但DOI确定不变。
更新日期/Last Update: 2018-11-19