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[1]许 萌陈 程谢 方罗洪镇丁 健史仲平*.以玉米粉/废弃毕赤酵母为混合原料高效发酵丁醇[J].应用与环境生物学报,2019,25(03):1-8.[doi:10.19675/j.cnki.1006-687x.2018.08006]
 XU Meng,CHEN Cheng,XIE Fang,et al.Effective butanol fermentation using corn starch and waste Pichia pastoris semi-solid mixture as the substrate*[J].Chinese Journal of Applied & Environmental Biology,2019,25(03):1-8.[doi:10.19675/j.cnki.1006-687x.2018.08006]
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以玉米粉/废弃毕赤酵母为混合原料高效发酵丁醇()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年03期
页码:
1-8
栏目:
研究论文
出版日期:
2019-06-25

文章信息/Info

Title:
Effective butanol fermentation using corn starch and waste Pichia pastoris semi-solid mixture as the substrate*
文章编号:
201808006
作者:
许 萌1陈 程1谢 方1罗洪镇2丁 健1史仲平1*
1江南大学生物工程学院、工业生物技术教育部重点实验室无锡 2141222淮阴工学院生命科学与食品工程学院淮安 223003
Author(s):
XU Meng1 CHEN Cheng1 XIE Fang1 LUO Hongzhen2 DING Jian1 & SHI Zhongping1**
1 The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China 2 School of Life Science & Food Engineering, Huaiyin Institute of Technology, Huaian 223003, Jiangsu, China
关键词:
ABE发酵丙酮丁醇梭菌丁醇固体废弃物资源化/减量化糖利用效率 Q815
Keywords:
ABE fermentation butanol Clostridium acetobutylicum waste solids recirculation & reduction starch sugar utilization yield
DOI:
10.19675/j.cnki.1006-687x.2018.08006
摘要:
丙丁梭菌发酵生产ABE(丙酮丁醇乙醇)溶剂的传统原料是玉米淀粉价格贵生产。重组毕赤酵母废弃物含有丰富的碳水化合物和蛋白质,有望替代淀粉原料。本研究用NaOH处理固态废弃毕赤酵母形成酵母液降低初始玉米粉用量、并在ABE发酵进入到产溶剂期后添加液对初始玉米粉用量、废酵母NaOH投料量进行了优化,最佳用量分别为8%、80 g/L、610 g/L。在此条件下,100 mL厌氧瓶下的丁醇产量可以稳定在912.0 g/L的较高水平,比8%玉米粉培养基对照提高80120%,与15%玉米粉培养基对照;与对照相比,总糖的利用效率可以从50%大幅提高到超过90%的水平;玉米粉用量可以节省57%以上;酵母废弃物有5%的碳水化合物可以有效地转化成气体/液体产物。以玉米粉/废弃毕赤酵母为混合原料发酵丁醇,可以同时实现固体废物的资源化和减量化、原料的节省化,提高了ABE发酵的经济性能。
Abstract:
Developing a novel corn-starch/waste P. pastoris semi-solid mixture substrate system for ABE fermentation process can increase the lower sugar utilization yield in traditional ABE fermentation, save raw material usage dose/cost and realize amount reduction and reutilization of waste P. pastoris semi-solid. Using NaOH to treat semi-solid waste yeast semi-solid to form solid/liquid suspensions, and then regulating pH of the suspensions back to 5-6 using H2SO4. Reducing the initial corn-starch content (from 15% to 8%) to start the fermentation, then adding the pretreated suspensions into broth when ABE fermentation entered solventogenesis phase ((24 h) by supplementing the suspension with the volume ratio of 1:4 (suspension/initial broth). Fermentations were implemented either in 100 mL anaerobic bottles or in a 7 L anaerobic fermentor. The optimal usage doses of initial corn-starch, waste yeast suspension and NaOH were identified as 8%, 80 g/L and 6-10 g/L, respectively. With the mixture substrate system, butanol concentration reached and stabilized around 9.0-12.0 g/L, which was 80%-120% higher than that obtained with 8% corn-starch based substrate and equivalently high compared with that using 15% corn-starch based substrate; total sugar utilization yield was largely increased from below 50% to more than 90%; corn starch was saved more than 57%; maximum 52% carbohydrate in the waste yeast semi-solid could be effectively reutilized or digested for gasification/liquefaction. A novel corn-starch/waste yeast semi-solid mixture substrate based ABE fermentation process was proposed. This system consumed a maximum 52% carbohydrate in the semi-solid waste for gasification/liquid products formation to reduce waste semi-solid amount; increased total sugar yield from 50% (controls) to over 90%; stably maintained ABE/butanol concentrations at least over 85% of the levels using 15% corn based substrate; saved over 57% expensive corn-starch usage doses, simultaneously.? ??

相似文献/References:

[1]吕彤,刘扬,赵青,等.烯酮还原酶基因的克隆与优化表达[J].应用与环境生物学报,2011,17(01):87.[doi:10.3724/SP.J.1145.2011.00087]
 LÜ,Tong,LIU Yang,et al.Cloning and Optimizing Expression of Enoate Reductase Gene from Clostridium acetobutylicum in Escherichia coli[J].Chinese Journal of Applied & Environmental Biology,2011,17(03):87.[doi:10.3724/SP.J.1145.2011.00087]
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备注/Memo

备注/Memo:
收稿日期 Received: 2018-08-07 接受日期 Accepted: 2018-09-19*江苏省自然科学基金(BK20170459)资助**通讯作者 (E-mail: zpshi@jiangnan.edu.cn)点击摘要页题目后的“PDF”可下载阅读全文;本文为已录用的作者修定稿,尚未经编辑全面修改。引用本文请注明出处本刊;发表刊期和页码将以正式出版时的安排为准,但DOI确定不变。
更新日期/Last Update: 2018-11-19