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[1]施华升 沈 仁豪 胡振 陈国炜 **.外源活性污泥胞外聚合物对小球衣藻聚集行为的影响*[J].应用与环境生物学报,2019,25(02):1-11.[doi:10.19675/j.cnki.1006-687x.2018.07027]
 SHI Huasheng,SHEN Renhao,HU Zhen CHEN Guowei,et al.The effect of activated sludge-extracted extracellular polymeric substance on the aggregation of Chlamydomonas microsphaera*[J].Chinese Journal of Applied & Environmental Biology,2019,25(02):1-11.[doi:10.19675/j.cnki.1006-687x.2018.07027]
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外源活性污泥胞外聚合物对小球衣藻聚集行为的影响*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
25卷
期数:
2019年02期
页码:
1-11
栏目:
研究论文
出版日期:
2019-04-25

文章信息/Info

Title:
The effect of activated sludge-extracted extracellular polymeric substance on the aggregation of Chlamydomonas microsphaera*
文章编号:
201807027
作者:
施华升1 沈 仁豪1 胡振 1 陈国炜 12**
1合肥工业大学土木与水利工程学院 合肥 230009
2河海大学水文水资源与水利工程科学国家重点实验室 南京 210098
Author(s):
SHI Huasheng1 SHEN Renhao1 HU Zhen1 CHEN Guowei1 2**?
1School of Civil and Hydraulic Engineering, Hefei University of Technology, Hefei 230009, China?
2State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering, Hohai University, Nanjing 210098, China
关键词:
微藻聚集污泥胞外聚合物细胞运动芳香族蛋白色氨酸类蛋白
Keywords:
microalgae aggregation EPS of activated slu dge cell motility aromatic protein tryptophan protein
DOI:
10.19675/j.cnki.1006-687x.2018.07027
摘要:
微藻聚集行为在藻华的治理等方面具有重要作用,也是目前微藻生物燃料收集的瓶颈之一。本文以小球衣藻为研究对象,观察污泥胞外聚合物(Extracellular polymeric substance,EPS)对微藻聚集行为的影响,并通过其聚集数据采集、EPS客观特性和微藻的自身响应等方面探究其可能的聚集原因。结果表明:随着外源性污泥EPS浓度的增大,小球衣藻聚集率从18.4%上升到81.8%,并且在110 mg/L时达到聚集率最大值。微藻细胞运动速度从26.8 μm/s上升到49.4 μm/s,增大了细胞间的碰撞几率,促进了微藻的聚集。三维荧光光谱表明,污泥胞外聚合物刺激了微藻色氨酸类蛋白和芳香族类蛋白的分泌,其分泌量与EPS的浓度成正比。但是外源性EPS溶液Zeta电位变化与微藻的聚集率呈负相关关系,并非微藻聚集原因。本研究表明,外源性污泥胞外聚合物能够促进微藻的聚集,研究结果可以为下一步在分子方面的探究提供实验基础。(图7 表3 参38)
Abstract:
Objectives: Though extracellular polymeric substances ( EPSs) play an important role in the aggregation of activated sludge, little is known about its effects on the aggregation of other non-bacterial species. In this study, the interaction between activ ated sludge -extracted EPSs and microalgae cells was investigated .Methods: Image and video analysis were applied in microalgae aggregation and motility tracking. The characteristics of EPSs and the self-response of microalgae were measured, too .Results: Results showed that with the increase of exogeneous EPS, the aggregation rate of Chlamydomonas microsphaera increased from 18.4% to 81.8%, reached the maximum aggregation rate at 110 mg/L. In the presence of activated sludge EPS, microalgae cells moved faster, with the speed increased from 26.8 μm/s to 49.4 μm/s, causing high collision probability among microalgae cells that favored the microalgae aggregation. The results of EEM also showed that EPS content was proportional to that of aromatic protein and tryptophan protein secreted by microalgae implied that sludge EPS stimulated the secretion of microalgae aromatic protein and t ryptophan protein. While the zeta potential of exogeneous EPS solution was negatively correlated to the aggregation rate of microalgae, indicat ing that zeta potential changes was not responsible for the accumulation of microalgae. Conclusions: Sludge EPS enhanced the aggregation of microalgae.? ?

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备注/Memo

备注/Memo:
收稿日期: 2018-07-20 接受日期 Accepted: 2018-09-27*国家自然科学基金项目(51479046)、水文水资源与水利工程科学国家重点实验室开放研究基金项目(2017490111)资助 **通讯作者(E-mail: gwchen@hfut.edu.cn)点击摘要页题目后的“PDF”可下载阅读全文;本文为已录用的作者修定稿,尚未经编辑全面修改。引用本文请注明出处本刊;发表刊期和页码将以正式出版时的安排为准,但DOI确定不变。
更新日期/Last Update: 2018-11-14